芝麻素对小鼠早期胚胎体外发育的影响

doi:10.16431/j.cnki.1671-7236.2019.12.025

Abstract

Abstract: The aim of this study was to investigate the effect of sesamin (SES) added to in vitro culture (IVC) on the development of early mice embryos.The fertilized eggs of 6-week-old female Kunming mice were randomly divided into control group and different concentrations (10,50,100 μmol/L) SES groups.The apoptosis rate and total cell number in blastocysts were detected by Fluorescein-dUTP and Hoechst 33342 immunofluorescence staining,respectively.DCFH-DA was used to detect the levels of reactive oxygen species (ROS) in early embryos;The levels of glutathione (GSH) in early embryos were detected by CMF₂HC;Early embryonic mitochondrial membrane potential intensity was measured using JC-1.The results showed that the segregation rate and blastocyst rate of SES groups were higher than those in control group,but there was no significant difference (P>0.05).The number of cells in 50 μmol/L SES group was significantly higher than that in control group (P<0.05),and the apoptosis rate was significantly lower than that in control group (P<0.05).Compared with control group,the ROS level in the 50 μmol/L SES group was significantly lower (P<0.05),and the GSH level and mitochondrial membrane potential level were significantly increased (P<0.05).The results showed that the addition of SES in IVC could increase the number of cells in blastocyst,decrease the apoptotic rate and the ROS level,increase the GSH level,improve the mitochondrial function of early embryos,reduce the damage of oxidative stress of embryos,and improve the quality of early embryonic development in mice.

Key words: mice; embryonic development; sesamin; ROS; mitochondrial membrane potential

CLC Number:

S814.6

HE Daohong, HAN Guobo, SUN Jingyu, E Zhiqiang, CUI Yuxin, XU Yongnan, GAO Qingshan. Effect of Sesamin on the Development of Early Mice Embryo in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3666-3673.

References

[1] GUERIN P,EL MOUATASSIM S,MENEZO Y.Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings[J].Human Reproduction Update,2001,7(2):175-189.
[2] ZHAO M H,LIANG S,KIM S H,et al.Fe(Ⅲ) is essential for porcine embryonic development via mitochondrial function maintenance[J].PLoS One,2015,10(7):e0130791.
[3] AGARWAL A,DURAIRAJANAYAGAM D,DU P S.Utility of antioxidants during assisted reproductive techniques:An evidence based review[J].Reproductive Biology and Endocrinology,2014,12(1):112.
[4] MORITA Y,TILLY J L.Oocyte apoptosis:Like sand through an hourglass[J].Developmental Biology,1999,213(1):1-17.
[5] MAKKER K,AGARWAL A,SHARMA R.Oxidative stress & male infertility[J].Indian Journal of Medical Research,2009,129(4):357-368.
[6] WANG L Y,WANG D H,ZOU X Y,et al.Mitochondrial functions on oocytes and preimplantation embryos[J].Journal of Zhejiang University Science B,2009,10(7):483-492.
[7] RAMALHO S J,AMARAL S,OLIVEIRA P J.Diabetes and the impairment of reproductive function:Possible role of mitochondria and reactive oxygen species[J].Current Diabetes Reviews,2008,4(1):46-54.
[8] MENEZO Y,DALE B,COHEN M.DNA damage and repair in human oocytes and embryos:A review[J].Zygote,2010,18(4):357-365.
[9] PARAMIO M T,IZQUIERDO D.Current status of in vitro embryo production in sheep and goats[J].Reproduction in Domestic Animals,2014,49(4):37-48.
[10] 高婷,王子旭,陈耀星,等.ROS介导的氧化应激与自噬[J].中国畜牧兽医,2018,45(3):656-662. GAO T,WANG Z X,CHEN Y X,et al.Oxidative stress and autophagy mediated by reactive oxygen species[J].China Animal Husbandry & Veterinary Medicine,2018,45(3):656-662.(in Chinese)
[11] 赵予晗,姚雪瑞,姜文杰,等.添加Ge-132对牛体外胚胎发育潜能的影响[J].中国畜牧兽医,2018,45(9):2537-2543. ZHAO Y H,YAO X R,JIANG W J,et al.Effect of adding Ge-132 on bovine embryo development potential in vitro[J].China Animal Husbandry & Veterinary Medicine,2018,45(9):2537-2543.(in Chinese)
[12] TAKAHASHI M.Oxidative stress and redox regulation on in vitro development of mammalian embryos[J].Journal of Reproduction and Development,2012,58(1):1-9.
[13] DENG S,ZHOU J L,FANG H S,et al.Sesamin protects the femoral head from osteonecrosis by inhibiting ROS-induced osteoblast apoptosis in rat model[J].Frontiers in Physiology,2018,9:1787.
[14] ZHANG F,CHU C H,XU Q,et al.A new amide from Asarum forbesii Maxim[J].Journal of Asian Natural Products Research,2005,7(1):1-5.
[15] COONEY R V,CUSTER L J,OKINAKA L,et al.Effects of dietary sesame seeds on plasma tocopherol levels[J].Nutrition and Cancer,2001,39(1):66-71.
[16] NOGUCHI T,IKEDA K,SASAKI Y,et al.Effects of vitamin E and sesamin on hypertension and cerebral thrombogenesis in stroke-prone spontaneously hypertensive rats[J].Hypertension Research:Official Journal of the Japanese Society of Hypertension,2001,24(6):735-742.
[17] LEE C C,CHEN P R,LIN S,et al.Sesamin induces nitric oxide and decreases endothelin-1 production in HUVECs:Possible implications for its antihypertensive effect[J].Journal of Hypertens,2004,22:2329-2338.
[18] BOURNIVAL J,PLOUFFE M,RENAUD J,et al.Quercetin and sesamin protect dopaminergic cells from MPP⁺ -induced neuroinflammation in a microglial (N9)-neuronal (PC12) coculture system[J].Oxidative Medicine and Cellular Longevity,2012,2012:921-941.
[19] CHING H L,MEI L S,SHUNG T K,et al.The effect of sesamin on airway fibrosis in vitro and in vivo[J].International Immunopharmacology,2014,22(1):141-150.
[20] XU Z,LIU Y,YANG D,et al.Sesamin protects SH-SY5Y cells against mechanical stretch injury and promoting cell survival[J].BMC Neuroscience,2017,18(1):57.
[21] MORANDI E,VERSTAPPEN R,ZWIERZINA M,et al.ITGAV and ITGA5 diversely regulate proliferation and adipogenic differentiation of human adipose derived stem cells[J].Scientific Reports,2016,6(2):88-89.
[22] 龙民慧.谷胱甘肽在N-乙酰半胱氨酸调控猪小肠上皮细胞生长中的作用[A].中国畜牧兽医学会动物营养学分会:第七届中国饲料营养学术研讨会[C].2014. LONG M H.Effect of glutathione on N-acetylcysteine regulating the growth of porcine intestinal epithelial cells[A].Society of Animal Nutrition,Chinese Society of Animal Husbandry and Veterinary Medicine:Seventh Chinese Symposium on Feed Nutrition[C].2014.(in Chinese)
[23] TARIN J J.Potential effects of age-associated oxidative stress on mammalian oocytes/embryos[J].Molecular Human Reproduction,1996,2(10):717-724.
[24] KROEMER G,GALLUZZI L,BRENNER C.Mitochondrial membrane permeabilization in cell death[J].Physiological Reviews,2007,87(1):99-163.
[25] 王华清,高青山,姜文杰,等.Psammaplin A对牛老化卵母细胞体外发育的影响[J].中国畜牧兽医,2019,46(2):497-503. WANG H Q,GAO Q S,JIANG W J,et al.Effect of Psammaplin A on in vitro development of bovine aging oocytes[J].China Animal Husbandry & Veterinary Medicine,2019,46(2):497-503.(in Chinese)

Effect of Sesamin on the Development of Early Mice Embryo in vitro

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