梅花鹿SCF基因参与色素合成的作用研究

doi:10.16431/j.cnki.1671-7236.2023.09.022

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the influence of stem cell factor (SCF) on the pigment synthesis process of sika deer.【Method】 Wild type (SCF1-9) and mutant (SCF789) expression vectors of sika deer SCF gene were constructed and transfected into HEK293 cells.Mouse melanoma cells (B16) were cultured with HEK293 cell culture medium to simulate the transport process of SCF in the body,and the melanin content and tyrosinase activity of B16 cells were detected.RT-PCR was used to detect the mRNA expression of microeye transcription factor M type (MITF-M),tyrosinase (TYR),tyrosinase-associated protein 1 (TYRP1),TYRP2,solute carry family 45 member 2 (SLC45A2),SRY box transcription factor 10 (SOX10) and cAMP response element-binding protein (CREB).The protein expression levels of MITF-M and TYR were detected by Western boltting.【Result】 Compared with control and SCF789 groups,the melanin content,tyrosinase activity,mRNA expression of MITF-M, TYR and SOX10 genes,and protein expression of TYR and MITF-M in SCF1-9 group were extremely significantly or significantly increased (P<0.01 or P<0.05).The mRNA expression of SCL45A2 gene in SCF1-9 group was significantly higher than that in SCF789 group (P<0.05),had no significant difference with control group (P>0.05).【Conclusion】 The SCF gene of sika deer regulated expression of pigment genes MITF-M,SOX10,TYR and SLC45A2 through SCF/c-kit signaling pathway to promote pigment synthesis.The results provided theoretical basis for further study on the expression regulation of pigment synthesis related genes.

Key words: sika deer; SCF gene; expression vector; melanocyte

CLC Number:

S825

CHEN Xu, DONG Yimeng, XING Xiumei, YANG Sukun, LIU Xin. Study on the Role of SCF Gene in Pigment Synthesis in Sika Deer[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3663-3670.

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Study on the Role of SCF Gene in Pigment Synthesis in Sika Deer

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