赤水乌骨鸡EDNRB2基因多态性及其与肤色性状的关联分析

doi:10.16431/j.cnki.1671-7236.2025.02.026

Abstract

Abstract: 【Objective】 This study was aimed to analyze the effects of polymorphisms in endothelin receptor B subtype 2 (EDNRB2) gene on skin color traits in Chishui Black-bone chickens,and screen the key polymorphism loci of EDNRB2 gene in regulating skin color of Chishui Black-bone chickens,in order to provide new genetic markers for a thorough examination of the intrinsic mechanism regulating melanin deposition in silky fowls and the optimization and improvement of skin color traits in Chishui Black-bone chickens. 【Method】 Chishui Black-bone chickens were used as the research subject in this study.The nine exons of EDNRB2 gene were sequenced after PCR amplification,the single nucleotide polymorphism (SNP) of EDNRB2 gene were identified by comparison,and the genetic diversity of EDNRB2 gene were analyzed.The mRNA secondary structure before and after the mutation of EDNRB2 gene SNPs was analyzed by RNAfold web server.The chain disequilibrium,haplotype and diplotype of EDNRB2 gene SNP were analyzed using SHEsis online website.The correlation between EDNRB2 gene SNP and skin color traits in Chishui Black-bone chickens was analyzed by SPSS 27.0 software. 【Result】 Five SNPs were identified in exons 3 and 9 of EDNRB2 gene in Chishui Black-bone chickens,of which two SNPs of g.11120193 T＞C and g.11120382 C＞T were found to exist in exon 3,and of which three SNPs of g.11125882 G＞A,g.11125917 T＞A and g.11125931 C＞A were found to exist in exon 9.There were three genotypes of TT,TC and CC in g.11120193 T＞C,and there were two genotypes of CC and CT in g.11120382 C＞T,which belonged to samesense mutation.There were three genotypes of GG,AG and AA in g.11125882 G＞A,which belonged to missense mutation (Gly→Glu).There were three genotypes of TT,AT and AA in g.11125917 T＞A,which belonged to missense mutation (Phe→Ile).There were three genotypes of CC,AC and AA in g.11125931 C＞A,which belonged to nonsense mutation.Five SNPs of EDNRB2 gene had a total of 10 haplotypes and 24 diplotypes,with H4 and H4H4 being the dominant haplotype and diplotype,respectively.The results of association analysis showed that the cockscomb △a value of CC genotype of EDNRB2 gene in g.11120193 T＞C was significantly higher than that of TC genotype (P＜0.05),the back △a value was significantly lower than that of TT genotype (P＜0.05).The cockscomb △L value of CC genotype of EDNRB2 gene in g.11125931 C＞A was significantly higher than that of AC genotype (P＜0.05).At the remaining SNPs,there were no significant variations in skin color parameters across individuals with different genotypes (P＞0.05).The most noticeable effects on the skin color traits in Chishui Black-bone chickens were caused by haplotypes H5H5 and H7H7. 【Conclusion】 EDNRB2 gene had five SNPs in exons 3 and 9,with g.11120193 T＞C and g.11125931 C＞A showing a substantial correlation with the skin color of cockscomb and back in Chishui Black-bone chickens.The haplotype H5H5 significantly impacted the cockscomb △L and △a values,back △b value,and underwing △b value.EDNRB2 gene could be utilized as a potential gene for breeding selection of skin color traits in Chishui Black-bone chickens.

Key words: Chishui Black-bone chickens; EDNRB2 gene; single nucleotide polymorphism; skin color; association analysis

CLC Number:

S813.3

YANG Jinlin, LI Hui, ZHAO Depeng, SHI Yushi, LONG Xia, TAN Qisong. Polymorphisms of EDNRB2 Gene and Its Association with Skin Color Traits in Chishui Black-bone Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 759-770.

References

[1] 叶涛.鸡SLCO1B3基因多态性研究及其互作蛋白筛选分析[D].贵阳:贵州大学,2022.YE T.Polymorphism of chicken SLCO1B3 gene and its interaction protein screening analysis[D].Guiyang:Guizhou University,2022.(in Chinese)
[2] 李思.赤水乌骨鸡种质特性及其黑色素保健价值的研究[D].贵阳:贵州大学,2015.LI S.Study on germplasm characteristics of Chishui Black-bone chickens and the value of health care of its melanin[D].Guiyang:Guizhou University,2015.(in Chinese)
[3] KAWASAKI-NISHIHARA A,NISHIHARA D,NAKAMURA H,et al.ET3/EDNRB2 signaling is critically involved in regulating melanophore migration in Xenopus[J]. Developmental Dynamics,2011,240(6):1454-1466.
[4] LIU H,LUO Q,ZHANG J,et al.Endothelins (EDN1,EDN2,EDN3) and their receptors (EDNRA,EDNRB,EDNRB2) in chickens:Functional analysis and tissue distribution[J]. General and Comparative Endocrinology,2019,283:113231.
[5] LI D,WANG X,FU Y,et al.Transcriptome analysis of the breast muscle of Xichuan Black-bone chickens under tyrosine supplementation revealed the mechanism of tyrosine-induced melanin deposition[J].Frontiers in Genetics,2019,10:457.
[6] ZI X,GE X,ZHU Y,et al.Transcriptome profile analysis identifies candidate genes for the melanin pigmentation of skin in Tengchong Snow chickens[J].Veterinary Sciences,2023,10(5)：341.
[7] YANG Y,WANG C,LIU Y,et al.Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser)[J]. British Poultry Science,2024,65(4):387-393.
[8] MAO N N,WANG W J,ZHANG R,et al.Population genomics reveals that a missense mutation in EDNRB2 contributes to white plumage color in pigeons[J].Poultry Science,2024,103(1):103225.
[9] MACLARY E T,WAUER R,PHILLIPS B,et al.An allelic series at the EDNRB2 locus controls diverse piebalding patterns in the domestic pigeon[J].PLoS Genetics,2023,19(10):e1010880.
[10] YANG Y,WANG H,LI G,et al.Using comparative genomics to detect mutations regulating plumage variations in Graylag (A.anser) and Swan geese (A.cygnoides)[J].Gene,2022,834:146612.
[11] GUO Q,JIANG Y,WANG Z,et al.Genome-wide association study for screening and identifying potential shin color loci in ducks[J].Genes,2022,13(8)：1391.
[12] GUO Q,JIANG Y,WANG Z,et al.Genome-wide analysis identifies candidate genes encoding beak color of duck[J].Genes,2022,13(7)：1271.
[13] MACLARY E T,PHILLIPS B,WAUER R,et al.Two genomic loci control three eye colors in the domestic pigeon (Columba livia)[J].Molecular Biology and Evolution,2021,38(12):5376-5390.
[14] KINOSHITA K,AKIYAMA T,MIZUTANI M,et al.Endothelin receptor B2(EDNRB2) is responsible for the tyrosinase-independent recessive white (mo(w)) and mottled (mo) plumage phenotypes in the chicken[J].PLoS One,2014,9(1):e86361.
[15] 刘航,王欢欢,葛莹,等.基于转录组和蛋白组筛选乌骨鸡肤色性状候选基因[J].畜牧兽医学报,2023,54(6):2320-2329.LIU H,WANG H H,GE Y,et al.Screening of candidate genes of skin color of Black-bone chicken based on transcriptome and proteome[J].Acta Veterinaria et Zootechnica Sinica,2023,54(6):2320-2329.(in Chinese)
[16] XU M,TANG S,LIU X,et al.Genes influencing deposition of melanin in breast muscle of the Xuefeng Black bone chicken based on bioinformatic analysis[J].Genome,2023,66(8):212-223.
[17] WU N,QIN H,WANG M,et al.Variations in endothelin receptor B subtype 2(EDNRB2) coding sequences and mRNA expression levels in 4 Muscovy duck plumage colour phenotypes[J].British Poultry Science,2017,58(2):116-121.
[18] MIWA M,INOUE-MURAYAMA M,AOKI H,et al.Endothelin receptor B2(EDNRB2) is associated with the panda plumage colour mutation in Japanese quail[J].Animal Genetics,2007,38(2):103-108.
[19] 屠云洁,栾德琴,章明,等.ALDH7A1和EDNRB2基因分型及其与乌骨鸡皮肤乌色度关联分析[J].中国畜牧兽医,2024,51(7):2923-2932.TU Y J,LUAN D Q,ZHANG M,et al.Genotyping of ALDH7A1 and EDNRB2 genes and their association with skin blackness in Black-bone chickens[J].China Animal Husbandry & Veterinary Medicine,2024，51(7):2923-2932.(in Chinese)
[20] 叶涛,李辉,罗韦,等.赤水乌骨鸡SLCO1B3基因SNPs鉴定及与蛋品质的关联性分析[J].畜牧与兽医,2023,55(2):25-31.YE T,LI H,LUO W,et al.SNPs identification of the SLCO1B3 gene and its correlation with the egg quality of Chishui Black-bone chicken[J].Animal Husbandry & Veterinary Medicine,2023,55(2):25-31.(in Chinese)
[21] HARRIS M L,HALL R,ERICKSON C A.Directing pathfinding along the dorsolateral path—The role of EDNRB2 and EPHB2 in overcoming inhibition[J].Development,2008,135(24):4113-4122.
[22] PLA P,ALBERTI C,SOLOV’EVA O,et al.EDNRB2 orients cell migration towards the dorsolateral neural crest pathway and promotes melanocyte differentiation[J].Pigment Cell Research,2005,18(3):181-187.
[23] 王琛.mRNA二级结构自由能在不同物种中的分布差异研究[D].杨凌:西北农林科技大学,2015.WANG C.Studies on the difference of mRNA secondary structure and MFE among different species[D].Yangling:Northwest A&F University,2015.(in Chinese)
[24] 毛圆辉.翻译过程中mRNA二级结构的功能研究[D].杨凌:西北农林科技大学,2015.MAO Y H.A systematic analysis of roles of mRNA secondary structures during translation[D].Yangling:Northwest A&F University,2015.(in Chinese)
[25] LIU C,ZOU Q,TANG H,et al.Melanin nanoparticles alleviate sepsis-induced myocardial injury by suppressing ferroptosis and inflammation[J].Bioactive Materials,2023,24:313-321.
[26] GUO K,JIAO Z,ZHAO X,et al.Melanin-based immunoregulatory nanohybrids enhance antitumor immune responses in breast cancer mouse model[J].ACS Nano,2023,17(11):10792-10805.
[27] 杨亚杰,李慧,胡清清,等.乌骨鸡黑色素的体外抗氧化活性及其对人脐静脉内皮细胞氧化损伤的保护作用[J].南昌大学学报(理科版),2019,43(1):59-64.YANG Y J,LI H,HU Q Q,et al.Protective effect of melanin from Black-bone Sliky fowl on human umbilical vein endothelial cell injury and antioxidant activity in vitro[J].Journal of Nanchang University(Natural Science), 2019,43(1):59-64.(in Chinese)

Polymorphisms of EDNRB2 Gene and Its Association with Skin Color Traits in Chishui Black-bone Chickens

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Yong, YING Qixin, LI Wei, XIONG Li, YANG Hongwen, WANG Chunyuan, WU Yan, XIANG Jin, ZHANG Yiyu. Polymorphisms of KRT10 Gene and Its Association Analysis with Reproductive Traits in Kele Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2508-2516.
[2]	XIANG Jin, YANG Qixin, LUO Hualun, HONG Yating, GUO Xiaojiang, YANG Hongwen, LI Wei, WANG Chunyuan, WU Yan, ZHANG Yiyu. Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1142-1150.
[3]	ZHENG Chunting, HU Yunyang, ZHANG Chunguang, YE Ziyuan, ZHANG Chen, LUO Yunyan, QIN Lei, PENG Zixuan, YU Yifei, XIAN Wangying, CHU Han, HONG Liang, ZHANG Jianbin, PU Lei. Research on Identification Method of Foreign Ternary Purebred, Binary,Ternary and Multiple Backcross Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1160-1170.
[4]	BI Yazhen, SHANG Mingyu, XIONG Jinke, HU Wenping, HE Jianning, ZHANG Li. Polymorphism of SRP68 Gene and Its Association with Growth Traits in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3189-3198.
[5]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[6]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[7]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[8]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[9]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[10]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[11]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[12]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[13]	QIANG Hao, LIANG Peng, MENG Ke, RONG Xuan, FENG Dengzhen. Polymorphism of IGF1R Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1765-1785.
[14]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[15]	LI Mingming, WU Qingwei, DENG Yuting, JIN Shengwei, HE Na, ZHANG Junxia. Correlation Analysis of BMPRⅡ Gene Polymorphism and Its Haplotype with Lambing Traits in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 587-597.