柯乐猪MMP2基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.03.026

Abstract

Abstract: 【Objective】 The experiment was conducted to explore single nucleotide polymorphisms (SNP) of matrix metallopeptidase 2 (MMP2) gene and its correlation with reproductive traits,and to screen genetic markers related to reproductive traits of Kele pigs.【Method】 A total of 212 healthy partriparous Kele sows were selected and the SNP of MMP2 gene were screened by direct sequencing.Using RNAfold online software to predict the secondary mRNA structure of different haplotypes of MMP2 gene.SPSS 22.0 software was used to analyze the correlation between MMP2 gene SNP and reproductive traits of Kele pigs.【Result】 1 SNP (g.30062403 C＞T) was detected in exon 2 and 2 SNPs (g.30065309 C＞T and g.30065312 C＞T) were detected in exon 4 of MMP2 gene,all with 3 genotypes (CC,CT and CT).The results of genetic analysis showed that g.30062403 C＞T was a low polymorphism,and g.30065309 C＞T and g.30065312 C＞T were moderate polymorphisms.Chi-square fitness test results showed that g.30062403 C＞T significantly deviated from Hardy-Weinberg equilibrium (P<0.05),and the other 2 SNPs were in Hardy-Weinberg equilibrium (P＞0.05).Linkage disequilibrium analysis showed that there was strong linkage disequilibrium between g.30065309 C＞T and g.30065312 C＞T.The analysis of haplotype mRNA secondary structure showed that the mRNA secondary structure of haplotype H1 was the same as the original sequence,and the other 3 haplotypes all caused changes in the mRNA secondary structure and minimum free energy.The results of association analysis showed that the litter number of TT genotype at g.30062403 C＞T was significantly higher than that of CC genotype,and the weaning litter weight was significantly higher than that of CT genotype (P<0.05).The number of weaned piglets of CC genotype at g.30065309 C＞T was significantly higher than that of TT genotype (P<0.05). The number of live litter and weaned piglets of TT genotype at g.30065312 C＞T was significantly higher than that of CT genotype (P<0.05).The diplotype H2H2 was the dominant diplotype of litter number,litter born alive,weaned piglets and weaning litter weight,while H1H4 was the dominant diplotype of litter weight at birth.【Conclusion】 There was a significant correlation between of MMP2 gene 3 SNPs and litter number,litter born alive,litter weight at birth,weaned piglets and litter weight at weaning,which could be used as genetic markers for molecular breeding.

Key words: Kele pigs; MMP2 gene; polymorphism; reproductive traits; association analysis

CLC Number:

S813.3

XIANG Jin, YANG Qixin, LUO Hualun, HONG Yating, GUO Xiaojiang, YANG Hongwen, LI Wei, WANG Chunyuan, WU Yan, ZHANG Yiyu. Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1142-1150.

References

[1] 李秀领,陈健梅,吴姿仪,等.全基因组关联分析鉴定法系大白猪繁殖性状基因组区域和候选基因[J].中国畜牧杂志, 2022, 58(8):157-164. LI X L, CHEN J M, WU Z Y, et al.Genome-wide association analysis identifies genomic regions and candidate genes for breeding traits in French White pigs[J].Chinese Journal of Animal Science, 2022, 58(8):157-164.(in Chinese)
[2] OGAWA S, KIMATA M, ISHII K, et al.Genetic analysis for sow stayability at different parities in purebred Landrace and Large White pigs[J].Animal Science Journal, 2021, 92(1):e13599.
[3] YE J, TAN C, HU X, et al.Genetic parameters for reproductive traits at different parities in Large White pigs[J].Journal of Animal Science, 2018, 96(4):1215-1220.
[4] 薛梅.分子育种技术在动物性状改造方面的应用[J].农业与技术, 2015, 35(19):128-129. XUE M.Application of molecular breeding techniques in the transformation of animal traits[J].Agriculture and Technology, 2015, 35(19):128-129.(in Chinese)
[5] SONG H, ZHANG J, JIANG Y, et al.Genomic prediction for growth and reproduction traits in pig using an admixed reference population[J].Journal of Animal Science, 2017, 95(8):3415-3424.
[6] ALI M A M, GARCIA-VILAS J A, CROMWELL C R, et al.Matrix metalloproteinase-2 mediates ribosomal RNA transcription by cleaving nucleolar histones[J].The FEBS Journal, 2021, 288(23):6736-6751.
[7] MYERS J E, MERCHANT S J, MACLEOD M, et al.MMP-2 levels are elevated in the plasma of women who subsequently develop preeclampsia[J].Hypertension in Pregnancy, 2005, 24(2):103-115.
[8] GOLDMAN S, SHALEV E.MMPS and TIMPS in ovarian physiology and pathophysiology[J]. Frontiers in Bioscience, 2004, 9:2474-2483.
[9] 孔亚茹,周小枫,熊浩铭,等.基质金属蛋白酶MMP2和MMP9在哺乳动物卵泡发育中的作用研究进展[J].广东农业科学, 2020, 47(3):103-110. KONG Y R, ZHOU X F, XIONG H M, et al.Research progress in effects of matrix metalloproteinases MMP2 and MMP9 on follicular development in mammals[J].Guangdong Agricultural Science, 2020, 47(3):103-110.(in Chinese)
[10] FORTUNATO S J, MENON R, LOMBARDI S J.Collagenolytic enzymes (gelatinases) and their inhibitors in human amniochorionic membrane[J].American Journal of Obstetrics and Gynecology, 1997, 177(4):731-741.
[11] FORTUNATO S J, MENON R, BRYANT C, et al.Programmed cell death (apoptosis) as a possible pathway to metalloproteinase activation and fetal membrane degradation in premature rupture of membranes[J].American Journal of Obstetrics and Gynecology, 2000, 182(6):1468-1476.
[12] ULRICH C C, ARINZE V, WANDSCHEER C B, et al.Matrix metalloproteinases 2 and 9 are elevated in human preterm laboring uterine myometrium and exacerbate uterine contractility[J].Biology of Reproduction, 2019, 100(6):1597-1604.
[13] LI X, JIN L, TAN Y.Different roles of matrix metalloproteinase 2 in osteolysis of skeletal dysplasia and bone metastasis (review)[J].Molecular Medicine Reports, 2021, 23(1):70.
[14] INOUE K, MIKUNI-TAKAGAKI Y, OIKAWA K, et al.A crucial role for matrix metalloproteinase 2 in osteocytic canalicular formation and bone metabolism[J].The Journal of Biological Chemistry, 2006, 281(44):33814-33824.
[15] BAUTERS D, SCROYEN I, VAN HUL M, et al.Gelatinase A (MMP-2) promotes murine adipogenesis[J].Biochimica et Biophysica Acta, 2015, 1850(7):1449-1456.
[16] LESNIAK A, HRABIA A.Expression of matrix metalloproteinase-2 mRNA in the chicken ovary in relation to follicle remodelling[J].Folia Biologica, 2012, 60(3-4):219-225.
[17] GOTTSCH M L, VAN KIRK E A, MURDOCH W J.Role of matrix metalloproteinase 2 in the ovulatory folliculo-luteal transition of ewes[J].Reproduction, 2002, 124(3):347-352.
[18] RILEY SC, WEBB C J, LEASK R, et al.Involvement of matrix metalloproteinases 2 and 9, tissue inhibitor of metalloproteinases and apoptosis in tissue remodelling in the sheep placenta[J]. Journal of Reproduction and Fertility, 2000, 118(1):19-27.
[19] UEKITA T, YAMANOUCHI K, SATO H, et al.Expression and localization of matrix metalloproteinases (MT1-MMP, MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) during synepitheliochorial placentation of goats (Capra hircus)[J].Placenta, 2004, 25(10):810-819.
[20] SEVAL Y, AKKYUNLU G, DEMIR R, et al.Distribution patterns of matrix metalloproteinase (MMP)-2 and-9 and their inhibitors (TIMP-1 and TIMP-2) in the human decidua during early pregnancy[J].Acta Histochemica, 2004, 106(5):353-362.
[21] KIZAKI K, USHIZAWA K, TAKAHASHI T, et al.Gelatinase (MMP-2 and-9) expression profiles during gestation in the bovine endometrium[J].Reproductive Riology and Endocrinology, 2008, 6:66.
[22] FERRER M, RODRIGUEZ H, ZARA L, et al.MMP2 and acrosin are major proteinases associated with the inner acrosomal membrane and may cooperate in sperm penetration of the zona pellucida during fertilization[J].Cell and Tissue Research, 2012, 349(3):881-895.
[23] YANG W J, LIU F C, HSIEH J S, et al.Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles[J].Reproductive Biology and Endocrinology, 2015, 13:102.
[24] HOWARD E W, CRIDER B J, UPDIKE D L, et al.MMP-2 expression by fibroblasts is suppressed by the myofibroblast phenotype[J]. Experimental Cell Research, 2012, 318(13):1542-1553.
[25] SLAVIK L, PROCHAZKOVA J, PROCHAZKA M, et al.The pathophysiology of endothelial function in pregnancy and the usefulness of endothelial markers[J].Biomedical Papers of the Medical Faculty of the University Palacky, 2011, 155(4):333-337.
[26] SERROTE C M L, REINIGER L R S, SILVA K B, et al.Determining the polymorphism information content of a molecular marker[J].Gene, 2020, 726:144175.
[27] WIGGINTON J E, CUTLER D J, ABECASIS G R.A note on exact tests of Hardy-Weinberg equilibrium[J].American Journal of Human Genetics, 2005, 76(5):887-893.
[28] LIU H, HU Y.Hardy-Weinberg equilibrium in genetic epidemiology[J].Zhong Nan Da Xue Xue Bao Yi Xue Bao, 2010, 35(1):90-93.
[29] DU F X, CLUTTER A C, LOHUIS M M.Characterizing linkage disequilibrium in pig populations[J].International Journal of Biological Sciences, 2007, 3(3):166-178.
[30] GREMLICH S, NGUYEN D, REYMONDIN D, et al.Fetal MMP2/MMP9 polymorphisms and intrauterine growth restriction risk[J].Journal of Reproductive Immunology, 2007, 74(1-2):143-151.

Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	BAI Jingjing, LIU Xiaoyu, LIU Lina, SONG Yanfeng, ZHANG Xin, SONG Xiaoyue, SHI Lei, LI Longping, ZHANG Lei, ZHU Haijing, QU Lei. Polymorphism of Notch2 Gene and Its Association Analysis with Cashmere and Growth Traits in Shaanbei White Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3630-3640.
[2]	BI Yazhen, SHANG Mingyu, XIONG Jinke, HU Wenping, HE Jianning, ZHANG Li. Polymorphism of SRP68 Gene and Its Association with Growth Traits in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3189-3198.
[3]	TAO Maohai, MENG Ke, RONG Xuan, QIANG Hao, NIE Wei, GUO Chenhao, FENG Dengzhen. Polymorphism of MSTN Gene and Its Association Analysis with Meat Quality Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2766-2776.
[4]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[5]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[6]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[7]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[8]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[9]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[10]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[11]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.
[12]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[13]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[14]	XUE Qian, LI Guohui, XING Weijie, ZHOU Chenghao, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, CAO Yuxia, SU Yijun, HAN Wei. Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2176-2185.
[15]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.