陕北白绒山羊Notch2基因多态性与绒毛和生长性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2023.09.019

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the association between insertion/deletion (InDel) mutation of Notch2 gene with cashmere and growth traits in Shaanbei White cashmere goats,in order to provide a theoretical basis for molecular breeding of goats.【Method】 A total of 1 469 adult female Shaanbei White cashmere goats were selected as the research objects,the ear tissue were collected to extract DNA,and their cashmere and growth traits,such as cashmere length,wool length,body length,body height were measured.The primers were designed in reference to the InDel genetic variation information of Notch2 gene in goats found in GenBank and Ensembl databases,and the 17 InDel mutations of Notch2 gene were detected by PCR amplification and direct sequencing,and the association between mutation and cashmere and growth traits in Shaanbei White cashmere goats was analyzed.【Result】 There were two polymorphism loci in 12 bp InDel(rs665021370) of intron 15 and 21 bp InDel(rs653705114) of intron 25 in Shaanbei White cashmere goats.Three genotypes of insertion/insertion type (II),heterozygous type (ID) and deletion/deletion type (DD) were present at the two InDels,which were all in low polymorphism (P＜0.25) and in Hardy-Weinberg equilibrium (P＞0.05).The association analysis results showed that rs665021370 mutation of Notch2 gene was significantly or extremely significantly correlated with body height and body length in Shaanbei White cashmere goats (P＜0.05 or P＜0.01);rs653705114 mutation was significantly or extremely significantly correlated with wool length,chest width and body height in Shaanbei White cashmere goats (P＜0.05 or P＜0.01).There was no strong linkage relationship between two InDels.【Conclusion】 rs665021370 and rs653705114 mutations of Notch2 gene had significant effect on cashmere and growth traits in Shaanbei White cashmere goats,which could be used as molecular markers for excellent trait breeding in Shaanbei White cashmere goats.

Key words: Shaanbei White cashmere goats; Notch2 gene; InDel; polymorphism; association

CLC Number:

S813.3

BAI Jingjing, LIU Xiaoyu, LIU Lina, SONG Yanfeng, ZHANG Xin, SONG Xiaoyue, SHI Lei, LI Longping, ZHANG Lei, ZHU Haijing, QU Lei. Polymorphism of Notch2 Gene and Its Association Analysis with Cashmere and Growth Traits in Shaanbei White Cashmere Goats[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3630-3640.

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Polymorphism of Notch2 Gene and Its Association Analysis with Cashmere and Growth Traits in Shaanbei White Cashmere Goats

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