柯乐猪KRT10基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.06.025

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effect of the polymorphism of keratin 10 (KRT10) gene on reproductive traits in Kele pigs,in order to improve the fertility of Kele pigs.【Method】 There were 255 Kele pigs used in this experiment.The single nucleotide polymorphism (SNP) of KRT10 gene was identified using a combination of PCR product sequencing,DANMAN sequence alignment software and manual verification.The population genetic characteristics of SNP were analyzed by SHEsis online software,and the bioinformatics analysis of SNP was conducted by RNAfold online tool.The general linear model of SPSS 22.0 software was used to analyze the correlation between SNP of KRT10 gene and reproductive traits in Kele pigs.【Result】 Three SNPs were identified in KRT10 gene of Kele pigs:g.21643703 C＞T and g.21643714 G＞A in intron 4,and g.21643741 G＞A in exon 5.g.21643741 G＞A mutation caused a codon mutation from AAG to AAA,encoding amino acids of lysine (K),which was a synonymous mutation and caused a change of the secondary structure in mRNA.Three genotypes were detected at all three mutation sites.g.21643703 C＞T and g.21643741 G＞A belonged to moderate polymorphism sites (0.25＜PIC＜0.5),and g.21643714 G＞A belonged to low polymorphism site (PIC＜0.25).g.21643703 C＞T and g.21643741 G＞A were in Hardy-Weinberg equilibrium (P＞0.05),g.21643714 G＞A was significantly deviated from Hardy-Weinberg equilibrium (P＜0.05).There were no strong linkage imbalance among three SNPs.Three SNPs produced a total of 4 haplotypes and 10 diploids.The results of correlation analysis showed that the effect of g.21643703 C＞T on newborn litter weight,weaned piglets and weaned litter weight in Kele pigs reached a significant level (P＜0.05),the effect of g.21643714 G＞A on total litter size and weaned litter weight in Kele pigs reached a significant level (P＜0.05),and the effect of g.21643741 G＞A on total litter size,live litter size,weaned piglets and weaned litter weight in Kele pigs reached a significant level (P＜0.05).The diploid H3H3 had the most significant effects on total litter size,live litter size,weaned piglets and weaned litter weight in Kele pigs.【Conclusion】 Three SNPs of KRT10 gene had significant effects on reproductive traits in Kele pigs,and H3H3 was advantageous diplotype,which might be used as a genetic marker for reproductive trait selection in Kele pigs.

Key words: Kele pigs; KRT10 gene; single nucleotide polymorphism (SNP); reproductive traits

CLC Number:

S813.3

ZHAO Yong, YING Qixin, LI Wei, XIONG Li, YANG Hongwen, WANG Chunyuan, WU Yan, XIANG Jin, ZHANG Yiyu. Polymorphisms of KRT10 Gene and Its Association Analysis with Reproductive Traits in Kele Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2508-2516.

References

[1] TERRINONI A,DIDONA B,CAPORALI S,et al.Role of the keratin 1 and keratin 10 tails in the pathogenesis of ichthyosis hystrix of Curth Macklin[J].PLoS One,2018,13(4):e0195792.
[2] 郭金竹,铁璐,王文慧.角蛋白10的功能与相关皮肤病[J].中国皮肤性病学杂志,2022,36(1):118-122. GUO J Z,TIE L,WANG W H.The function and related dermatosis of keratin 10[J]. Chinese Journal of Dermatology and Venereology,2019,36(1):118-122.(in Chinese)
[3] SARAH K,SUSANNE Å,VIDHYA J,et al.Heterozygous KRT10 missense variant in a Chihuahua with severe epidermolytic ichthyosis[J].Animal Genetics,2023,54(5):652-654.
[4] ÅGREN M S,LITMAN T,ERIKSEN J O,et al.Gene expression linked to reepithelialization of human skin wounds[J].International Journal of Molecular Sciences,2022,23(24):15746.
[5] 甘奕传,张三泉,黎小东,等.角蛋白10突变致先天性大疱性鱼鳞病样红皮病1例[J].皮肤性病诊疗学杂志,2022,29(6):532-536. GAN Y C,ZHANG S Q,LI X D,et al.A case report of congenital bullous ichthyosiform erythroderma caused by a mutation of KRT10 gene[J].Journal of Dermatology and Venereology,2022,29(6):532-536.(in Chinese)
[6] ZHANG J,LUO R C,MAN X Y.The anatomy of the skin of the Chinese tree shrew is very similar to that of human skin[J].Zoological Research,2020,41(2):208-212.
[7] 牛艳芳.TRIM21通过泛素化稳定细胞骨架蛋白KRT10促进下咽癌分化的机制研究[D].太原:山西医科大学,2022. NIU Y F.TRIM21 promotes differentiation of hypopharyngeal squamous cell carcinoma through improving protein stability by ubiquitination of cytoskeleton protein KRT10[D].Taiyuan: Shanxi Medical University,2022.(in Chinese)
[8] HUANG X,YIN G,ZHOU B,et al,et al.KRT10 plays an important role in the release of viral genome from endosomes during H9N2 subtype AIV replication in HeLa cells[J].Veterinary Microbiology,2023,284:109824.
[9] JOVANDARIC M Z,MILENKOVIC S J.Congenital ichthyosis (icthyosis lamellaris non bullosa)-therapy[J].Journal of Obstetrics and Gynaecology,2021,41(4):655-656.
[10] KURZ B,KOSCHITZKI K T,HEHR U,et al.Congenital ichthyosiform erythroderma with epidermolysis due to a novel frameshift mutation in KRT10[J].Journal of the American Academy of Dermatology, 2023,35:74-76.
[11] MARCH O P,LETTNER T,KLAUSEGGER A,et al.Gene editing-mediated disruption of epidermolytic ichthyosis-associated KRT10 alleles restores filament stability in keratinocytes[J].Journal of Investigative Dermatology,2019,139(8):1699-1710.
[12] CUNHA G R,KURITA T,CAO M,et al.Molecular mechanisms of development of the human fetal female reproductive tract[J]. Differentiation,2017,97:54-72.
[13] KABASHIMA K,MATSUZAKI M,SUZUKI H.Intermediate filament keratin dynamics during oocyte maturation requires maturation/mphase promoting factor and mitogen-activated protein kinase kinase activities in the hamster[J].Reproduction in Domestic Animals,2010,45(5):e184-e188.
[14] ZHANG C,WU Z,HU G,et al.Exploring characteristics of placental transcriptome and cord serum metabolome associated with low birth weight in Kele pigs[J].TropAnimal Health Production,2023,55(5):340.
[15] 胡光玲,申涛,伍治敏,等.柯乐猪和大白猪胎盘发育与繁殖性能的相关性分析[J].畜牧与兽医,2023,55(6):1-10. HU G L,SHEN T,WU Z M,et al.Correlation analysis of placental development and reproductive performance in Kele pigs and Yorkshire pigs[J].Animal Husbandry & Veterinary Medicine,2023,55(6):1-10.(in Chinese)
[16] 王春源,熊力,杨红文,等.柯乐猪异常乳腺组织的TMT蛋白组学分析[J].福建农林大学学报(自然科学版),2024,53(1):69-78. WANG C Y,XIONG L,YANG H W,et al.Proteomic analysis of abnormal mammary gland tissue of Kele pig based on TMT technique[J].Journal of Fujian A&F University (Natural Science Edition),2024,53(1):69-78.(in Chinese)
[17] 杨酸,郭小江,杨红文,等.柯乐猪PRLR基因多态性与繁殖性状的关联性[J].浙江农业学报,2023,35(3):556-564. YANG S,GUO X J,YANG H W,et al.Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig[J]. Journal of Zhejiang Agricultural Sciences,2023,35(3):556-564.(in Chinese)
[18] SMEDTS F,RAMAEKERS F,LEUBE R E,et al.Expression of keratins 1,6,15,16,and 20 in normal cervical epithelium,squamous metaplasia,cervical intraepithelial neoplasia,and cervical carcinoma[J].American Journal of Pathology,1993,142(2):403-412.
[19] WAUTERS C C,SMEDTS F,GERRITS L G,et al.Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary[J].Human Pathlogy,1995,26(8):852-855.
[20] RICCIARDELLI C,LOKAN N A,PYRAQYUS C E,et al.Keratin 5 overexpression is associated with serous ovarian cancer recurrence and chemotherapy resistance[J].Oncotarget,2017,8(11):17819-17832.
[21] WANG Y F,LANG H Y,YUAN J,et al.Overexpression of keratin 17 is associated with poor prognosis in epithelial ovarian cancer[J].Tumour Biology,2013,34(3):1685-1689.
[22] YIN S,LI Z,YANG F,et al.A comprehensive genomic analysis of Chinese indigenous Ningxiang pigs:Genomic breed compositions,runs of homozygosity,and beyond[J].International Journal of Molecular Sciences,2023,24(19):14550.
[23] LIU C,HUANG R,SU G,et al.Introgression of pigs in Taihu Lake region possibly contributed to the improvement of fertility in Danish Large White pigs[J].Biotechnology & Applied Microbiology Genomics,2023,24(1):733.
[24] HUO J L,ZHANG L Q,ZHANG X,et al.Genome-wide single nucleotide polymorphism array and whole-genome sequencing reveal the inbreeding progression of Banna Minipig inbred line[J].Animal Genetics, 2022,53(1):146-151.
[25] KIMURA M.A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J].Journal of Molecular Evolution,1980,16(2):111-120.
[26] STRAUSS B S.Hypermutability and silent mutations in human carcinogenesis[J]. Seminars in Cancer Biology,1998,8(6):431-438.
[27] LI M,LU X,DONG J,et al.A synonymous mutation in exon 39 of FBN1 causes exon skipping leading to Marfan syndrome[J].Genomics,2020,112(6):3856-3861.
[28] GOODMAN D B,CHURCH G M,KOSURI S.Causes and effects of N-terminal codon bias in bacterial genes[J].Science,2013,342(6157):475-479.
[29] DADOUSIS C,MUNOZ M,ÓVILO C,et al.Admixture and breed traceability in European indigenous pig breeds and wild boar using genome-wide SNP data[J].Scientific Reports,2022,12(1):7346.

Polymorphisms of KRT10 Gene and Its Association Analysis with Reproductive Traits in Kele Pigs

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