柯乐猪PAEP基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.04.027

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the influence of polymorphism in progestogen associated endometrial protein (PAEP) gene on reproductive traits in Kele pigs,with the objective of identifying genetic markers that could enhance their reproductive traits.【Method】 A total of 189 multiparous Kele pigs were used as experimental animals,the single nucleotide polymorphism (SNP) were identified in PAEP gene through PCR product sequencing and sequence alignment.The SHEsis online software was utilized to analyze the population genetic characteristics of SNP.Bioinformatics analysis of SNP was carried out using RNAfold,SOPMA,SWISS-MODEL and SWISS-PdbViewer programs.The SPSS 22.0 software was employed to analyze the correlation between SNP of PAEP gene and reproductive traits in Kele pigs.【Result】 Three SNP sites were identified in PAEP gene of Kele pigs,a missense mutation g.1884992 T＞C in exon 4,resulting in the change of valine (V) at position 135 to alanine (A),as well as mutations in intron 4:g.1885152 G＞C and g.1887834 G＞A.All three SNPs displayed three genotypes.g.1884992 T＞C was low polymorphism (PIC＜0.25),while g.1885152 G＞C and g.1887834 G＞A were moderate polymorphism (0.25＜PIC＜0.5).The genotype distribution of g.1884992 T＞C did not deviate from Hardy-Weinberg equilibrium (P＞0.05),while the genotype distribution of g.1885152 G＞C and g.1887834 G＞A were extremely significantly deviated from Hardy-Weinberg equilibrium (P＜0.01).Linkage analysis revealed that there was complete linkage between g.1885152 G＞C and g.1887834 G＞A,resulting in the generation of 3 haplotypes and 6 diplotypes.g.1884992 T＞C resulted in the changes of the secondary structure in mRNA and protein of PAEP gene.The association analysis showed that g.1884992 T＞C was significantly affected total litter size and total number born in Kele pigs (P＜0.05),while g.1885152 G＞C and g.1887834 G＞A were significantly affected total litter size,total number born,number of weaned and weaning litter weight in Kele pigs (P＜0.05).The total litter size,total number born,number of weaned and weaning litter weight of H3H3 diplotype in Kele pigs were significantly higher than those of other 5 diplotypes (P＜0.05),and the birth weight of H3H3 diplotype in Kele pigs was significantly higher than that of H1H1,H2H2 and H2H3 diplotypes (P＜0.05).The total number born and number of weaned of H1H3 diplotype were significantly higher than those of H1H1,H1H2,H2H2 and H2H3 diplotypes (P＜0.05),and the total number born of H1H3 diplotype in Kele pigs was significantly higher than that of H2H3 diplotype (P＜0.05).【Conclusion】 Three SNPs found in PAEP gene significantly impacted the reproductive traits in Kele pigs.H3H3 was advantageous diplotype,and might serve as a genetic marker for the selection of reproductive traits in Kele pigs.

Key words: Kele pigs; PAEP gene; SNP; reproductive traits

CLC Number:

S813.3

ZHAO Yong, GUO Xiaojiang, LI Wei, XIONG Li, YANG Hongwen, WANG Chunyuan, WU Yan, XIANG Jin, ZHANG Yiyu. Polymorphism of PAEP Gene and Its Association with Reproductive Traits in Kele Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1593-1602.

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Polymorphism of PAEP Gene and Its Association with Reproductive Traits in Kele Pigs

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