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Study on Genetic Diversity of Domestic Chicken in Guizhou

WANG Wen-tao1,LIN Jia-dong2,CHEN Juan-hua2,ZHANG Fu-ping2   

  1. (1. Qingzhen Agricultural Bureau, Guizhou Province, Qingzhen 551400, China;2. College of Animal Science, Guizhou University, Guiyang 550025,China)
  • Received:2013-10-18 Online:2014-04-20 Published:2014-05-27

Abstract: PCR and direct sequencing methods were used to analyze the genetic diversity and systematic evolution in Guizhou domestic fowls by determining a 780 bp DNA fragment of control region of mtDNA from 90 individuals of 6 Guizhou domestic chicken breeds. The percentage of nucleotide A, G, C and T were 26.14%,13.38%,25.98% and 34.50%, respectively. There were 40 polymorphic sites accounting for 5.13% of total analyzed sites. The diversity of haplotypes in 6 Guizhou domestic chicken breeds was ranged from 0.506 to 0.976, which showed significant genetic differentiation and higher levels of population genetic polymorphism.

Key words: Guizhou domestic chicken breeds; mtDNA D-loop; genetic diversity