Abstract: The material from the clinical samples of cerebrum and spleen in the pigs characterized by pseudorabies, proved to be porcine pseudorabies virus (PRV) positive samples through the amplification of gE gene of PRV, was inoculated into the BHK-21 cells and serially passaged 4 times. The isolate could induce typical cytopathogenic effect in cells and the titer of the fourth passage was 10^7.0 TCID_50/mL. Identification was done by PCR method and four rabbits were injected with the fourth generation cell cultures of the isolate, and the specific band could be amplified and the typical clinical syndrome could be observed. It was indicated that the isolated virus named ZJ strain was PRV. The virulent PRV ZJ strain was killed with formalin and used as vaccine antigen with oil adjuvant. The rabbits were injected with 1 mL vaccine, 28 days after inoculation, serum antibodies were detected and healthy rabbits were challenged by PRV ZJ virulent strain, and the protection rate and serum neutralization index of antibodies were tested. The results showed that protection rate and serum neutralization index in rabbits were 100% and 13490,respectively.This study showed that PRV ZJ strain had good immunogenicity, and provided a basis for further research on vaccination of PRV.

Key words: porcine pseudorabies virus; isolation and identification; inactivated vaccines; immunogenicity