鹿源藿香假丝酵母菌DZ018的分离鉴定及其体外益生特性研究

doi:10.16431/j.cnki.1671-7236.2025.01.035

Abstract

Abstract: 【Objective】 Yeast with probiotic potential was isolated and identified from the rumen fluid of sika deer,and its probiotic functions in vitro were evaluated to provide a reference for its application in animal nutrition and feed and the development of deer derived probiotic preparations. 【Method】 The yeast strain was isolated from rumen fluid of healthy male sika deer by YPD solid medium.The isolate was identified by morphological observation,physiological,biochemical and molecular biology.The growth performance and acid production were measured,and the growth curve was drawn.The tolerance of the isolate in different pH,different concentration of bile salts,artificial gastric fluid and artificial intestinal fluid was investigated.The drug sensitivity of the strain was detected by Kirby-Bauer (K-B) method,and the safety of the strain was tested in mice. 【Result】 A dominant yeast strain was isolated from rumen fluid of sika deer and named DZ018.The morphological characteristics,physiological and biochemical characteristics and ITS rRNA sequence of the colony were consistent with those of Candida rugosa.The results of growth performance test showed that the isolate grew rapidly in YPD liquid medium,entered logarithmic growth stage after cultured for 2 h,and had a certain ability to produce acid.The results of tolerance evaluation showed that the isolate had good tolerance to low pH,and could grow normally in the range of pH 2.5-6.5,and the optimal growth pH was 4.5.The isolate had strong tolerance to bile salts,and the survival rate was ＞100% in 0.3% and 1.0% bile salts,and the survival rate reached 74.27% in 2.0% bile salt.The survival rate of artificial gastric fluid and intestinal fluid was 31.64% and 64.80%,respectively.The results of drug sensitivity test showed that the isolate was sensitive to fluorocytosine,fluconazole and ketoconazole,and resistant to amphotericin B.The results of safety test in mice showed that the isolate had no adverse effects on the growth performance and organ coefficient of mice. 【Conclusion】 In this study,a strain of Candida rugosa DZ018 with probiotic potential was isolated from rumen fluid of sika deer,with strong tolerance and good safety.The experimental results provided reference and theoretical basis for the application of deer probiotics in the production of deer animals and the development of microecological preparations.

Key words: Candida rugosa; sika deer; isolation and identification; probiotic properties

CLC Number:

S811.6

HUANG Yao, YAO Naiquan, YUAN Weitao, MAO Aipeng, ZHANG Ting, XU Chao. Isolation and Identification of Candida rugosa DZ018 from Deer and Its Probiotic Properties in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 389-399.

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Isolation and Identification of Candida rugosa DZ018 from Deer and Its Probiotic Properties in vitro

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