犬脂肪间充质干细胞的分离培养及分化潜能研究

Abstract

Abstract: In order to obtain canine adipose-derived mesenchymal stem cells (ASCs), the dog inguinal subcutaneous adipose tissue was used to isolate ASCs by the methods of tissue culture and enzyme digestion, respectively. Different cell morphologies and proliferation characteristics were observed and the differentiation potential was tested by inducing cells into osteoblasts and adipocytes. The results showed that the adipose tissue from young dogs could get a lot of ASCs by tissue culture methods, which grew strong, uniform morphology, and could differentiate into osteoblasts with alkaline phosphatase positive and adipocytes with oil red O positive. The isolation and culture method of canine ASCs was easy to be established and could provide sufficient cell sources for the study of cells transplantation and therapy.

Key words: culture in vitro; differentiation potential; adipose-derived mesenchymal stem cells; canine

GE Xiu-guo, LI Ji-xia, JIN Tian-ming, MA Ji-fei. Isolation, Culture and Differentiation Potential of Canine Adipose-derived Mesenchymal Stem Cells[J]. .

References

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Isolation, Culture and Differentiation Potential of Canine Adipose-derived Mesenchymal Stem Cells

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