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Research on Genetic Effects of Myogenin Gene SNPs on the Reproductive Traits in Jinghai Yellow Chicken

TANG Ying1,2, WANG Jin-yu1,2, ZHANG Gen-xi1, 2, GE Ying1, 2, XUE Qian1,2, WANG Ya-nan1,2, WANG Yong-juan3   

  1. (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; 3.Jiangsu Jinghai Poultry Industry Group Co., Ltd., Nantong 226103, China)
  • Received:2013-10-23 Online:2014-04-20 Published:2014-05-27

Abstract: The objective of this study was to analyze the association of single nucleotide polymorphisms (SNPs) in MyoG gene with chicken reproductive traits. In this study, PCR-SSCP approach was applied to assess SNPs of MyoG. Genetic effects were analyzed between different genotypes and reproductive traits of 378 Jinghai Yellow chicken by using the least square analysis. The results showed that sequencing revealed one mutation (G102A) on exon 1 of MyoG gene in the genotype AA in comparison to the genotype BB while other mutation (T36C) on exon 3 of MyoG gene in the genotype CC in comparison to the genotype DD. The least square analysis showed that there was no significant difference among genotype AA,AB and BB (P>0.05). While individuals with DD genotype had advantages on weight at first egg than those with CC genotype (P<0.05). In other reproductive traits, there was no significant difference among genotype CC,CD and DD (P>0.05). It suggested that SNPs of MyoG gene had no significant effects on reproductive traits of Jinghai Yellow chicken and which could be used in marker-assisted selection to accelerate the chicken breeding progress.

Key words: myogenin gene; polymorphism; Jinghai Yellow chicken; reproductive traits; correlation