鱼源山梨醇发酵阳性温和气单胞菌的分离与鉴定

Abstract

Abstract: A bacteria was isolated from the intestine of Carassius auratus and temporarily named as SR-1 strain with sorbitol-positive character. It was identified as Aeromonas sobria by morphological, physiological, biochemical characteristics, drug sennsitivity test, 16S rDNA sequence and phylogenetic tree analysis. Further the 16S rDNA sequence of SR-1 was amplified by PCR method and the results showed that the size of fragment was 1509 bp. Phylogenetic tree analysis showed that SR-1 strain had the highest similarity to Aeromonas sobria of 99.87% to 100%. Drug sensitivity test results revealed that SR-1 was sensitive to azithromycin, chloroamphenicol, tetracycline, norfloxacin, cefotaxime， cefixime and so on.

Key words: Carassius auratus; Aeromonas sobria; isolation and identification; drug sensitivity test; phylogenetic tree

LI Xu， DING Chu-chu, LI Xue, SHI Hui, CHEN Ye-xiang, HU Xiu-cai, ZHU Ai-hua, LV Ai-jun. Isolation and Identification of Sorbitol-positive Aeromonas sobria from Fish[J]. .

References

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Isolation and Identification of Sorbitol-positive Aeromonas sobria from Fish

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