珲春敬信湿地候鸟白额雁恶臭假单胞菌的分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2025.01.042

Abstract

Abstract: 【Objective】 This study was aimed to understand the China-Russia-North Korea border area prevalence of pathogenic bacteria carried by migratory birds in Hunchun Jingxin wetland,so as to provide scientific basis for migratory bird disease prevention and control. 【Method】 This study collected 155 fresh fecal samples from the migratory bird species Anser albifrons located at the Hunchun Jingxin wetland.The collected Anser albifrons fecal samples were diluted in PBS and cultured on LB agar and blood agar media.Individual colonies were isolated and purified,and the bacteria were identified using Gram staining,biochemical tests,PCR amplification and sequencing of the 16S rRNA gene,and other methods. The drug resistance of the isolated bacteria was analyzed. 【Result】 The isolated bacteria were Gram negative bacteria,appearing as purple red short rod-shaped bacteria.Biochemical tests of the isolate strain revealed positive results for urease,lysine decarboxylase,ornithine decarboxylase,semisolid,etc.,and hydrogen sulfide,phenylalanine,gluconate,etc.were negative.A single band with a size of 1 500 bp was amplified by PCR,and the amplified sequence had the highest similarity with the sequence of Pseudomonas aeruginosa MZ836867 published in NCBI,which was 99.6%.Phylogenetic analysis revealed that the isolated strain belonged to the same branch with strain MZ836867.This indicated that the isolated strain was Pseudomonas putidis.The results of drug sensitivity test showed that the isolate was sensitive to 13 kinds of drugs,such as chloramphenicol,cotrimoxazole and norfloxacin,intermediated to 9 kinds of drugs such as polycolistin B,clindamycin and imipenem,and it was resistant to 8 drugs including vancomycin,flufenicol, lincomycin and oxacillin, etc.,among which lincomycin and oxacillin were the most resistant. 【Conclusion】 This study successfully isolated multidrug-resistant Pseudomonas putida from the fecal samples of Anser albifrons in Hunchun Jingxin wetland,providing a reference for further research on the gut microbiota of migratory birds in this wetland.

Key words: Hunchun; Anser albifrons; Pseudomonas putida; isolation and identification; drug resistance

CLC Number:

S852.61

MA Zhen, WANG Longsheng, TANG Zeyu, MIN Pengfei, ZHAO Jianhao, MENG Fanqi, XUE Shujiang, JIA Lijun. Isolation,Identification and Drug Sensitivity Analysis of Pseudomonas putidis from Anser albifrons in Hunchun Jingxin Wetland[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 461-469.

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Isolation,Identification and Drug Sensitivity Analysis of Pseudomonas putidis from Anser albifrons in Hunchun Jingxin Wetland

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