林麝源肺炎克雷伯菌耐药性及毒力基因分析

doi:10.16431/j.cnki.1671-7236.2025.01.037

Abstract

Abstract: 【Objective】 The resistance and virulence genes of Klebsiella pneumoniae,which could easily cause pneumonia and other diseases of adult forest musk deer,were analyzed in a forest musk deer farm in Guangdong,so as to provide reference for effective prevention and control of diseases caused by Klebsiella pneumoniae. 【Method】 In this study,81 fresh feces of forest musk deer were collected from a forest musk deer ranch in Guangdong. Klebsiella pneumoniae was isolated and purified by isolation culture,morphological observation,biochemical analysis,and PCR amplification of 16S rRNA and hemolytic enzyme (khe) genes.The characteristics of isolates were studied by serotype identification,drug susceptibility test,drug resistance and virulence genes detection. 【Result】 The isolates formed pink,smooth and moist single colony on McConkey plate.The strain showed short red rod shape under the microscope after Gram-staining and was Gram-negative.Strains with biochemical characteristics consistent with Klebsiella pneumoniae were selected for PCR amplification.PCR amplification of 16S rRNA gene showed that a band with a size of about 1 542 bp was obtained.A total of 33 isolates showed a nucleotide similarity of over 98% with the 16S rRNA gene of Klebsiella pneumoniae in GenBank database,and the specific gene khe was detected as positive.Therefore,this study successfully isolated and identified 33 strains of Klebsiella pneumoniae from 81 samples.The serotype test results showed that the dominant serotype among the isolates was K57.The drug sensitivity results showed that the isolates exhibited varying degrees of resistance to penicillin,aminoglycosides,tetracyclines,sulfonamides,chloramphenicols,quinolones and monocyclic β-lactams.However,it was sensitive to cephalosporins and carbapenems.Among the 12 drug resistance genes detected,the detection rates of bla_SHV and oqxA genes were both 96.97%.The detection rates of aac(6')-Ⅰb-cr,rmtB,tetA,tetM,sul1,sul2,oqxB and floR genes were 57.58%,51.52%,78.79%,84.85%,72.73%,66.67%,87.88% and 51.52%,respectively.bla_KPC and bla_NDM genes were not detected.Among the 9 virulence genes detected,the detection rates of wabG,uge,mrkD,entB and ureA genes were 90.91%,96.97%,96.97%,100% and 100%,respectively.The detection rates of aerobactin and allS genes were 6.06% and 18.18%,respectively,and ybtA gene was not detected. 【Conclusion】 In this study,33 strains of Klebsiella pneumoniae were isolated and identified from forest musk deer.The dominant serotype was K57,which had multiple drug resistance and a high carrying rate of drug resistance genes and virulence genes.The results of this study could provide data support for the prevention and control of diseases caused by Klebsiella pneumoniae from forest musk deer.

Key words: Moschus berezovskii; Klebsiella pneumoniae; drug resistance; virulence genes; antibacterials

CLC Number:

S852.61

LIAO Huiqun, ZHAO Mei, ZENG Guohui, SU Renwei, DENG Xianbo. Analysis of Drug Resistance and Virulence Genes of Klebsiella pneumoniae from Moschus berezovskii[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 411-421.

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Analysis of Drug Resistance and Virulence Genes of Klebsiella pneumoniae from Moschus berezovskii

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