鹅源芽孢杆菌和乳酸菌耐药基因及生物学特性分析

doi:10.16431/j.cnki.1671-7236.2025.01.032

Abstract

Abstract: 【Objective】 The aim of this experiment was to study the biological characteristics of eight potential probiotic strains isolated from the intestinal tract of geese,and provide a reference for the subsequent development and utilization of the strains in a rational way. 【Method】 The strains were characterized by morphological observation,biochemical identification,PCR identification and 16S rRNA gene sequencing.Biological characterization of the strains included acid production,optimum growth temperature,acid resistance,bile salt resistance,bacteriostatic activity and enzyme production were tested.The drug resistance of the strains was analyzed by drug sensitivity test and drug resistance gene detection. 【Result】 Eight strains of bacteria isolated from goose intestines were named A1-A5 and X1-X3,respectively.Through morphological observation and biochemical identification,it was preliminarily determined that A1-A5 were Bacillus and X1-X3 were lactic acid bacteria.PCR amplification of 16S rRNA gene obtained bands of about 1 500 bp in all isolates.The sequence similarity alignment results of 16S rRNA gene showed that A1 and A2 were Bacillus subtilis,A3-A5 were Bacillus velezensis,Bacillus amyloliquefaciens and Bacillus licheniformis respectively,X1-X3 were Lactobacillus fermentum, Lactobacillus reuteri and Lacticaseibacillus paracase,respectively.The results of acid production performance showed that X1-X3 had strong acid production performance,and the acid production performance of X1 was the best,with the pH reaching 3.84 at 36 h.The optimal growth temperature of all eight strains of bacteria was 37 ℃.The results of acid and bile salt resistance test showed that all eight strains of bacteria had good tolerance,among which A2 had the best tolerance,with a survival rate of more than 70% when the pH was 4.0 and the bile salt concentration was 0.9%.The results of the bacterial inhibition test showed that all eight strains of bacteria had good bacterial inhibition,and the inhibition zone diameters were all above 10.00 mm,among which A2 had the best bacterial inhibition effect.The results of the enzyme production performance test showed that A1-A5 had strong enzyme production performance,and the enzyme production effect of A2 was the best,with the diameter of the amylase ring reaching 15.5 mm,and the diameter of the cellulase ring reaching 14.3 mm.The results of the drug sensitivity test showed that the eight strains of bacteria were more than moderately sensitive to most antibacterials.Resistance gene analysis showed that tetracycline gene TetA (25.0%),quinolone gene GyrA (12.5%) and macrolide gene ErmF (12.5%) were the major resistance genes,among which strain A3 carried tetracycline gene TetA (480 bp),strain A4 carried quinolone gene GyrA (382 bp) and tetracycline gene TetA (480 bp),and strain X1 carried the macrolide gene ErmF (306 bp). 【Conclusion】 Among the eight strains of bacteria isolated from the intestinal tract of geese,Bacillus subtilis A2 had strong probiotic properties and had the potential to be used as a microecological agent of goose origin.

Key words: Bacillus; lactic acid bacteria; growth characteristics; antibacterial properties; drug resistance gene

CLC Number:

S852.61

WAN Baoxia, MENG Lingying, SUN Siyu, ZHAO Yujie, WANG Jiaqi, WANG Qiuju. Analysis of Drug Resistance Genes and Biological Characterization of Goose Derived Bacillus and Lactic Acid Bacteria[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 351-363.

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Analysis of Drug Resistance Genes and Biological Characterization of Goose Derived Bacillus and Lactic Acid Bacteria

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