产细菌素贝莱斯芽孢杆菌CM7-4全基因组测序及抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2024.12.001

Abstract

Abstract: 【Objective】 Bacillus velezensis CM7-4 was isolated from seawater and had been demonstrated to possess good antibacterial activity.This study was to investigate the antimicrobial mechanism of Bacillus velezensis CM7-4 and analyze the whole genome sequence in order to explore its antibacterial and other functional genes.【Method】 The bacteriocin crude extract of Bacillus velezensis CM7-4 was obtained by acid precipitation,and the effect of proteinase K on its antibacterial effect was determined by the solid agar perforation method.Subsequently,the complete genome of Bacillus velezensis CM7-4 was sequenced on the MGI DNBSEQ-T7 platform.Their bacteriocin production potential was analyzed by sequence assembly,gene prediction and functional annotation.【Result】 The bacteriocin crude extract of Bacillus velezensis CM7-4 demonstrated a notable bacteriostatic effect on a diverse range of 8 kinds of Gram-negative and positive bacteria,including Escherichia coli,Salmonella,Enterococcus faecalis,Listeria monocytogenes and others. Compared with control group, the bacteriocin crude extract demonstrated a extremely significantly diminished inhibitory effect on the eight indicator bacteria following treatment with proteinase K (P＜0.01).Whole genome sequencing revealed that the genome size of Bacillus velezensis CM7-4 was 3 953 124 bp with a 47.04% GC content.A total of 3 870 protein-coding genes,9 rRNA, 81 tRNA and 1 ncRNA were identified.And 11 bacteriocin synthesis genes,10 antioxidant activity genes,and 4 immune and inflammatory signalling pathway regulation genes were predicted.【Conclusion】 The results of this experiment indicated that Bacillus velezensis CM7-4 was a potential bacteriocin-producing strain.This study provided a theoretical basis for further study of its antibacterial properties.

Key words: Bacillus; whole genome sequencing; bacteriocin; gene function annotation

CLC Number:

S816.7

MA Haotian, LI Yang, YU Mengbo, PAN Ruixue, LONG Yuner, ZHAO Yining, PENG Jinju, MA Yi. Whole Genome Sequencing of Bacteriocin-producing Bacillus velezensis CM7-4 and Its Bacteriostatic Activity[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(12): 5117-5127.

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Whole Genome Sequencing of Bacteriocin-producing Bacillus velezensis CM7-4 and Its Bacteriostatic Activity

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