Abstract: To study the effect of nerve growth factor （NGF） on proliferation of mouse ovarian granulosa cells. Primary mouse ovarian granulosa cells were cultured successfully, immunofluorescence was used to identify granulosa cells and observe the distributions of NGF and TrkA in granulosa cells, the MTS was used to analysis the different concentrations of NGF on proliferation of mouse granulosa cells. The cells were treated with NGF of different concentrations (10, 50, 100, 500 ng/mL) for 24 h. The D_{490 nm} value of the cell that was identified was determined by micro-plate reader.Compared with the control group, each treatment group promote the proliferation of granulosa cells with different degrees (P<0.05), the effect of NGF group （50 ng/mL） on the granulosa cells proliferation was the most significant (P<0.01). These results indicated that NGF could promote proliferation of mouse granulosa cells. Also,mouse ovarian granulosa cells could express NGF and TrkA.

Key words: ovarian granulosa cells; NGF; proliferation