绵羊和人主要组织相容性复合体DRB1基因外显子2多态性及生物信息学分析

Abstract

Abstract: This study was aimed to detect the single nucleotide polymorphisms (SNPs) and single amino acid polymorphisms (SAPs) of Ovis aries and Homo sapiens DRB1 genes exon2, and analyze the structure and characteristic change of OLA-DRB1 protein exon2 by bio-informatics. The sequence polymorphisms of Homo sapiens and Ovis aries MHC-DRB1 exon2 were analyzed with comparative genomics and biological databases. The protein structures and functions of Ovis aries MHC-DRB1 were predicted and analyzed with bio-informatics softwares. The result showed that there were rich polymorphisms and SAPs both in HLA-DRB1 and OLA-DRB1 exon2. The number of singleton polymorphic and parsimony informative polymorphic sites were different from each other. There were only 14 same SNPs loci compared with each other, the rest of the site were different. Sequence analysis indicated that they were highly homology on MHC-DRB1 exon2. The results of the bio-informatics analysis indicated that caused by sequence variation of single amino acid triggering protein structure’s change secondary structure and tertiary structure, could lead to obnormal gene function, and result in disease resistance and disease susceptibility mutation.

Key words: Ovis aries MHC-DRB1; Homo sapiens MHC-DRB1; single nucleotide polymorphisms; single amino acid polymorphisms; bio-informatics

CHEN Yue-e, LI Jian-hua, GOU Ya-feng, WANG Wen-wen, ZHU Jun-bao, GAO Jian-feng. Polymorphisms and Bio-informatics Analysis of Ovis aries and Homo sapiens DRB1 Genes Exon2[J]. .

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Polymorphisms and Bio-informatics Analysis of Ovis aries and Homo sapiens DRB1 Genes Exon2

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