猪流感病毒H1N1亚型天津分离株核蛋白、基质蛋白、非结构蛋白基因克隆与序列分析

Abstract

Abstract: Six strains of H1N1 subtype swine influenza viruses were isolated from pigs with influenza-like signs in Tianjin. Three pairs of primers were designed based on nucleoprotein (NP), matrix protein (M) and nonstructural protein (NS) genes of the influenza virus H1N1 subtype in GenBank. The NP, M and NS genes were amplified by RT-PCR, which were cloned into pMD18-T vector, sequenced and analyzed. As demonstrated by homology and phylogenic analysis, it was found that the NP, M and NS genes of A/swine/Tianjin/TJ2/2005 (H1N1) and A/swine/Tianjin/TJ4/2006 (H1N1) were higher homologues with A/swine/Guangdong/33/2006, belonging to the classical H1N1 swine lineage. While the A/swine/Tianjin/TJ3/2006 (H1N1) and A/swine/Tianjin/TJ8/2006 (H1N1) were higher homologues with A/Dunedin/2/2000 (H1N1), maybe belonging to human H1N1 lineage. A/swine/Tianjin/TJ6/2009 (H1N1) and A/swine/Tianjin/TJ7/2009 (H1N1) were higher homologues with A/swine/Jiangsu/s15/2011 (H1N1), belonging to the class of avian H1N1 swine lineage. This study showed the evolution and epidemic situation of swine influenza viruses in Tianjin area.

Key words: swine influenza; H1N1 subtype; nucleoprotein gene; matrix protein gene; nonstructural protein gene; sequence analysis

SUN Ying-feng, YAN Ming-hua, LU Chao, LI Xiu-li, ZHANG Li, HAN Wei, REN Wei-ke, TIAN Xiang-xue. Cloning and Sequence Analysis of Nucleoprotein, Matrix Protein and Nonstructural Protein Gene of H1N1 Subtype Swine Influenza Virus Isolated from Tianjin[J]. .

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Cloning and Sequence Analysis of Nucleoprotein, Matrix Protein and Nonstructural Protein Gene of H1N1 Subtype Swine Influenza Virus Isolated from Tianjin

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