牛结节性皮肤病病毒检测及蠓、蚊携带病毒调查

doi:10.16431/j.cnki.1671-7236.2023.10.032

Abstract

Abstract: 【Objective】 This study was aimed to understand the virus-carrying status of infected cattle with Lumpy skin disease virus (LSDV) under natural infection and the potential vectors of the virus,so as to provide reference for the prevention and control of lumpy skin disease.【Method】 The different tissue types of specimens were collected from cattle suspected infected with LSDV in Wenshan city,Yunnan province during July 2021.The midges and mosquitoes were collected from cattle feedlots which suspected infected with LSDV in Kunming,Wenshan city and Shuangjiang county,Yunnan province in August 2020 and July 2021 to August 2021,respectively,and the morphological identification was performed.The nucleic acid of LSDV in different tissue types of specimens of infected cattle and midges and mosquitoes were detected by Real-time quantitative PCR.PCR amplification and sequencing of the skin lesions specimens were carried out with specific primer pair of LSDV127 gene.The nucleotide homology and phylogenetic analysis were performed by DNAStar and Mega 6.0 softwares.【Result】 The test of LSDV in whole blood,serum,nasal swab and skin lesion specimens of three infected cattle were positive,and the signals of LSDV amplification curve were the strongest in skin lesion specimens.In addition,the result of LSDV127 gene sequence analysis showed that three positive of nucleic acid of LSDV in skin lesion specimens were 100% homogeneity,and had the closer genetic relationship and clustered together with other LSDV which isolated from Russia,Thailand,Vietnam and South Africa,and showed 99.7%-100% homogeneity.A total of 4 822 midges specimens were trapped and identified constituting 3 different species including C.tainanus,C.jacobsoni and C.oxystoma,as well as 150 specimens of mosquitoes. No specimens of midges and mosquitoes tested were positive for nucleic acid of LSDV.【Conclusion】 These results suggested that the different parts such as blood,nasal secretions and skin lesions of cattle which naturally infected with LSDV might provide a plentiful source of pathogens for arthropod vectors to acquire and spread LSDV,and it was necessary to further investigate whether arthropod vectors such as midges and mosquitoes were involved in the transmission of local LSDV.

Key words: Lumpy skin disease virus (LSDV); midge; mosquito; sequence analysis

CLC Number:

S852.65^＋3

LI Nan, MENG Jinxin, WANG Jiannie, HE Yuwen, YANG Shaochang, LYU Shunyan, WANG Jinglin. Detection of Lumpy Skin Disease Virus in Cattle and Investigation of Virus Carried by Midges and Mosquitoes[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4179-4187.

References

[1] LU G,XIE J X,LUO J L,et al.Lumpy skin disease outbreaks in China,since August 3,2019[J].Transboundary and Emerging Diseases,2021,68(2):216-219.
[2] ZHOU T,JIA H J,CHEM G H,et al.Phylogenetic analysis of Chinese Sheeppox and Goatpox virus isolates[J].Virology Journal,2012,9(1):25.
[3] 周雪梅,李应国,聂奎,等.牛结节性皮肤病及其病原检测方法研究进展[J].动物医学进展,2011,32(10):99-102. ZHOU X M,LI Y G,NIE K,et al.Progress on lumpy skin disease and detection methods of its pathogen[J].Progress in Veterinary Medicine,2011,32(10):99-102.(in Chinese)
[4] TUPPURAINEN E S M,VENTER E H,SHISLER J L,et al.Review:Capripoxvirus diseases:Current status and opportunities for control[J].Transboundary and Emerging Diseases,2017,64(3):729-745.
[5] BABIUK S,BOWDEN T R,PARKYN G,et al.Quantification of Lumpy skin disease virus following experimental infection in cattle[J].Transboundary and Emerging Diseases,2008,55(7):299-307.
[6] CASAL J,ALLEPUZ A,MITEVA A,et al.Economic cost of lumpy skin disease outbreaks in three Balkan countries:Albania,Bulgaria and the Former Yugoslav Republic of Macedonia (2016-2017)[J].Transboundary and Emerging Diseases,2018,65(6):1680-1688.
[7] 谢士杰,朱俊达,王天坤,等.牛结节性皮肤病疫苗研究进展[J].中国畜牧兽医,2021,48(11):4220-4230. XIE S J,ZHU J D,WANG T K,et al.Research progress on vaccine development for lumpy skin disease[J].China Animal Husbandry & Veterinary Medicine,2021,48(11):4220-4230.(in Chinese)
[8] SPRYGIN A,PESTOVA Y,WALLACE D B,et al.Transmission of Lumpy skin disease virus:A short review[J].Virus Research,2019,269:e197637.
[9] SPRYGIN A,PESTOVA Y,PRUTNIKOV P,et al.Detection of vaccine-like Lumpy skin disease virus in cattle and Musca domestica L.flies in an outbreak of lumpy skin disease in Russia in 2017[J].Transboundary and Emerging Diseases,2018,65(5):1137-1144.
[10] GUPTA T,PATIAL V,BALI D,et al.A review:Lumpy skin disease and its emergence in India[J].Veterinary Research Communications,2020,44(3-4):111-118.
[11] RATAN M,GIASUDDIN M,HASSAN M Z,et al.Isolation and molecular identification of Lumpy skin disease (LSD) virus from infected cattle in Bangladesh[J].Bangladesh Journal of Livestock Research,2019,26(1-2):15-20.
[12] TRAN H T T,TRUONG A D,DANG A K,et al.Lumpy skin disease outbreaks in Vietnam,2020[J].Transboundary and Emerging Diseases,2021,68(3):977-980.
[13] ARJKUMPA O,SUWANNABOON M,BOONROD M,et al.First emergence of lumpy skin disease in cattle in Thailand,2021[J].Transboundary and Emerging Diseases,2021,68(6):3002-3004.
[14] ODONCHIMEG M,ERDENECHIMEG D,TUVSHINBAYAR A,et al.Molecular identification and risk factor analysis of the first lumpy skin disease outbreak in cattle in Mongolia[J].Journal of Veterinary Medical Science,2022,84(9):1244-1252.
[15] 农业农村部.农业农村部关于做好牛结节性皮肤病防控工作的紧急通知.农牧发
[2019] 26 号[Z].2019. THE MINISTRY OF AGRICULTURE AND RURAL AFFAIRS,PRC.Urgent notice of the Ministry of Agriculture and Rural Affairs on the prevention and control of lumpy skin disease in cattle,No.26,2019.[Z].2019.(in Chinese)
[16] SOHIER C,HAEGEMAN A,MOSTIN L,et al.Experimental evidence of mechanical Lumpy skin disease virus transmission by Stomoxys calcitrans biting flies and Haematopota spp.horseflies[J].Scientific Reports,2019,9(1):20076.
[17] UPPURAINEN E S M,LUBINGA J C,STOLTSZ W H,et al.Evidence of vertical transmission of Lumpy skin disease virus in Rhipicephalus decoloratus ticks[J].Ticks and Tick-Borne Diseases,2013,4(4):329-333.
[18] CHIHOTA C M,RENNIE L F,KITCHING R P,et al.Mechanical transmission of Lumpy skin disease virus by Aedes aegypti (Diptera:Culicidae)[J].Epidemiology and Infection,2001,126(2):317-321.
[19] BERG C,BØTNER A,BROWMAN H,et al.Scientific opinion on lumpy skin disease[J].EFSA Journal,2015,13(1):3986.
[20] ISSIMOV A,KUTUMBETOV L,ORYNBAYEV M B,et al.Mechanical transmission of Lumpy skin disease virus by Stomoxys spp.(Stomoxys calsitrans,Stomoxys sitiens,Stomoxys indica),Diptera:Muscidae[J].Animals (Basel),2020,10(3):477.
[21] SPRYGIN A,ARTYUCHOVA E,BABIN Y,et al.Epidemiological characterization of lumpy skin disease outbreaks in Russia in 2016[J].Transboundary and Emerging Diseases,2018,65(6):1514-1521.
[22] MOLLA W,DE JONG M C M,FRANKENA K.Temporal and spatial distribution of lumpy skin disease outbreaks in Ethiopia in the period 2000 to 2015[J].BMC Veterinary Research,2017,13(1):310.
[23] KAHANA-SUTIN E,KLEMENT E,LENSKY I,et al.High relative abundance of the stable fly Stomoxys calcitrans is associated with lumpy skin disease outbreaks in Israeli dairy farms[J].Medical and Veterinary Entomology,2017,31(2):150-160.
[24] MAFIRAKUREVA P,SAIDI B,MBANGA J.Incidence and molecular characterisation of Lumpy skin disease virus in Zimbabwe using the P32 gene[J].Tropical Animal Health and Production,2017,49(1):47-54.
[25] PURSE B V,CARPENTER S,VENTER G J,et al.Bionomics of temperate and tropical Culicoides midges:Knowledge gaps and consequences for transmission of Culicoides-borne viruses[J].Annual Review of Entomology,2015,60(1):373-392.
[26] AUGOT D,HADJ-HENNI L,STRUTZ S E,et al.Culicoides association between host species choice and morphological characters of main sensory structures of in the Palaeartic region[J].PeerJ,2017,5:e3478.
[27] 杨振兴,寇美玲,廖德芳,等.2019年云南省景洪市哨兵动物多种虫媒病毒的分离鉴定[J].中国畜牧兽医,2020,47(12):4127-4137. YANG Z X,KOU M L,LIAO D F,et al.Isolation and identification of Arbovirus from the sentinel herds in Jinghong city,Yunnan province,China in 2019[J].China Animal Husbandry & Veterinary Medicine,2020,47(12):4127-4137.(in Chinese)
[28] 李占鸿,朱建波,寇美玲,等.1株库蠓源版纳病毒的分离与全基因组序列分析[J].中国畜牧兽医,2021,48(3):781-791. LI Z H,ZHU J B,KOU M L,et al.Isolation and whole genome sequence analysis of a Banna virus strain from Culicoides[J].China Animal Husbandry & Veterinary Medicine,2021,48(3):781-791.(in Chinese)
[29] CHIHOTA C M,RENNIE L F,KITCHING R P,et al.Attempted mechanical transmission of Lumpy skin disease virus by biting insects[J].Medical and Veterinary Entomology,2003,17(3):294-300.
[30] 国家市场监督管理总局,中国国家标准化管理委员会. GB/T 39602-2020牛结节性皮肤病诊断技术[S].北京:中国标准出版社,2020. STATE ADMINISTRATION FOR MARKET REGULATION, STANDARDIZATION ADMINI-STRATION. GB/T 39602-2020 Diagnostic techniques for lumpy skin disease[S]. Beijing:Standard Press of China,2020.(in Chinese)
[31] ADEDEJI A J,MÖLLER J,MESEKO C A,et al.Molecular characterization of Capripox viruses obtained from field outbreaks in Nigeria between 2000 and 2016[J].Transboundary and Emerging Diseases,2019,66(4):1631-1641.
[32] BEDEKOVIĆ T,ŠIMIĆ I,KREŠIĆ N,et al.Detection of Lumpy skin disease virus in skin lesions,blood,nasal swabs and milk following preventive vaccination[J].Transboundary and Emerging Diseases,2018,65(2):491-496.
[33] ŞEVIK M,DOǦAN M.Epidemiological and molecular studies on lumpy skin disease outbreaks in Turkey during 2014-2015[J].Transboundary and Emerging Diseases,2017,64(4):1268-1279.
[34] 陈云明,李锡慧,石凤海,等.云南省首例牛结节性皮肤病的紧急流行病学调查[J].中国动物检疫,2022,39(1):4. CHEN Y M,LI X H,SHI F H,et al.Emergency epidemiological investigation on the first case of lumpy skin disease in Yunnan province[J].China Animal Health Inspection,2022,39(1):4.(in Chinese)

Detection of Lumpy Skin Disease Virus in Cattle and Investigation of Virus Carried by Midges and Mosquitoes

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