黄麻羽肉鸡β-防御素基因AvBD1和AvBD2克隆、生物信息学分析及组织表达

doi:10.16431/j.cnki.1671-7236.2023.11.002

Abstract

Abstract: 【Objective】 Defensins are novel active peptides produced in vivo with broad spectrum antimicrobial activity.In-depth understanding of the basic information of β-defensins AvBD1 and AvBD2 in chickens will be helpful for further study and full application of their biological functions.【Method】 The sequences of AvBD1 and AvBD2 genes in Yellow-feather chickens were amplified using PCR, then cloned and sequenced, subsequently the CDS sequences obtained by sequencing were deduced as amino acid sequences for similarity alignment.The bioinformatics characteristics of the predicted proteins were analyzed by online software, and the expression of the corresponding encoding genes in different tissues of Yellow-feather chickens were compared.【Result】 The CDS length of AvBD1 and AvBD2 genes in Yellow-feather chickens were 198 and 195 bp, which encoded 65 and 64 amino acids, respectively.The similarity of CDS between AvBD1 and AvBD2 genes was 56%, and the similarity of amino acid sequences was 36%, indicating that the two were not homologous genes.The phylogenetic tree showed that Yellow-feather chickens had the closer genetic relationship with several other breeds of chickens, and AvBD1 and AvBD2 genes had the furthest genetic relationship with Mus musculus and Parus major, respectively.Bioinformatics analysis displayed that both AvBD1 and AvBD2 were hydrophobic proteins, and AvBD1 had stronger hydrophobicity and stability than AvBD2.They all contained signal peptides, however, they were located at different amino acid sites and there was no transmembrane domain.The secondary structure elements were mainly alpha helix and random coil, respectively, but the proportion of amino acids involved in the formation of the two conformations was significantly different.Real-time quantitative PCR results found that the overall expression trend of AvBD1 and AvBD2 genes were similar in heart, liver, lung, kidney and duodenum of Yellow-feather chickens, with the highest expression in lung and the lowest expression in duodenum.【Conclusion】 In this study, the CDS sequences of AvBD1 and AvBD2 genes in Yellow-feather chickens were cloned successfully, and the structure and function of the corresponding proteins were analyzed.Both of them were consistent at the mRNA level in visceral tissues of Yellow-feather chickens.The results laid a reference for further elucidating the biological function and application of AvBD1 and AvBD2 in production.

Key words: Yellow-feather chickens; AvBD1 gene; AvBD2 gene; cloning; sequence analysis; tissue expression

CLC Number:

Q78
S831

KANG Danju, PENG Qiuyun, ZHOU Jiaxin, CHEN Jinjun, LIN Hongying, ZHOU Guangxian. Cloning, Bioinformatics Analysis and Tissue Expression of AvBD1 and AvBD2 Genes in Yellow-feather Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4325-4337.

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Cloning, Bioinformatics Analysis and Tissue Expression of AvBD1 and AvBD2 Genes in Yellow-feather Chickens

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