1株诱发血管瘤的ALV-J的分离鉴定及其囊膜基因的进化分析

doi:10.16431/j.cnki.1671-7236.2023.11.024

Abstract

Abstract: 【Objective】 This study was aimed to investigate the origin of J-avian leukosis of a black-finned egg farm in Hubei and the evolutionary trend of its envelope gene, and provide information for the epidemiological investigation of avian leukosis in Hubei.【Method】 Pathological examination, ELISA and Multi-PCR were used for laboratory diagnosis of suspected cases of hemangioma J-Avian leucosis virus (ALV-J).Viral fluid was prepared and inoculated with DF-1 cells for virus isolation and indirect immunofluorescence (IFA) detection, and the gene encoding the envelope protein of this strain was analyzed by DNAStar and other softwares.【Result】 The case presented typical hemangioma typical symptomatic lesions, the p27 antigen test was positive, the Multi-PCR results showed ALV-J specific bands, the IFA results showed specific fluorescence, which indicated that 1 strain of ALV was isolated successfully, named HB2021017.The amino acid similarity were increased progressively between the envelope membrane protein ENV, membrane surface glycoprotein SU, transmembrane protein TM of the isolate HB2021017 and the corresponding proteins of the cited ALV-J strains of myeloblastoma, mixed myeloblastoma and hemangioma, and hemangioma-type ALV-J strains.Phylogenetic tree analysis showed that the env gene of the isolate HB2021017 was most closely related to the hemangioma-inducing ALV-J strain isolated from local breed chickens in China, and was in the same branch;While it was more distantly related to the hemangioma-inducing or myeloblastoma-inducing ALV-J strain isolated from other chickens, and was in different branches.【Conclution】 A strain of hemangioma-inducing ALV-J, named HB2021017, was isolated.The envelope genes of the hemangioma-inducing ALV-J strain isolated from local breed chickens had formed a relatively independent evolutionary branch.

Key words: hemangioma type; J-Avian leucosis virus (ALV-J); envelope gene; cloning; evolution

CLC Number:

S852.65⁺9.3

LI Youjun, WU Lan, YANG Yuxin, GU Yufang, FANG Chun, LIU Jing, LIANG Xiongyan, YANG Yuying. Isolation and Identification of a Hemangioma-inducing ALV-J Strain and Evolution Analysis of Envelope Genes[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4557-4567.

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Isolation and Identification of a Hemangioma-inducing ALV-J Strain and Evolution Analysis of Envelope Genes

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