China Animal Husbandry & Veterinary Medicine

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Analysis of Resistant Phenotypes and Resistant Genes of Pathogenic Salmonella from Avian

DAI Jian-hua1, WU Zhi1, YUAN Wei-feng2   

  1. (1.Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;2.Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193,China)
  • Received:2013-10-23 Online:2014-04-20 Published:2014-05-27

Abstract: To investigate the presence of antibiotic resistance and resistant genes about avian Salmonella in Suzhong area, 22 isolates were evaluated for antimicrobial sensitivity by K-B disc diffusion method against 12 antimicrobial drug,and then 12 resistant genes of the isolates were detected by PCR. The resistant rates of all isolates were found from 72.73% to 100% to amikacin, norfloxacin and so on; 5 resistant phenotypes were detected, and each isolates were resistant to more than 4 of the 12 antimicrobial drugs,even 10 drugs; 9 different resistant genes were amplified, the homologies were very high compared with the corresponding genes in GenBank (≥98%); the resistant phenotypes and their resistance related genes were essential consistent, but no absolute correlation. The above results showed that the antibiotic resistance of Salmonella was very serious, and the resistant genes existed widely.

Key words: avian; Salmonella; resistant phenotypes; resistant genes