RPA检测技术在禽源病原检测中的研究进展

doi:10.16431/j.cnki.1671-7236.2024.02.005

Abstract

Abstract: Recombinant polymerase amplification (RPA) is a new type of constant temperature nucleic acid amplification technology that has attracted more attention from researchers in recent years.Due to its convenient operation, fast amplification speed, high specificity, and high sensitivity, without the need for precision instruments, and being able to complete nucleic acid detection in a constant temperature environment (25-45 ℃), it is highly likely to replace traditional PCR detection technology.Since the development of RPA for over a decade, it has been widely applied in the fields of bacteria, viruses, fungi, parasites, and drug resistance.This method has been recognized by researchers as the most promising rapid molecular diagnostic tool currently, it has a wide range of application prospects.The author summarized the RPA detection, technological advantages, common detection methods, and research progress in the detection of avian pathogens, aiming to provide theoretical reference for the research of new detection technologies for clinical avian pathogens.

Key words: recombinant polymerase amplification (RPA); avian pathogens; constant temperature nucleic acid amplification technology

CLC Number:

S858.3

BAO Taotao, YANG Xianfu, LIAO Fei, WU Tongkui, MO Xinghu, WANG Zhongrong. Research Progress of RPA Detection Technology in the Detection of Avian Pathogens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 482-490.

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Research Progress of RPA Detection Technology in the Detection of Avian Pathogens

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