福建白羽肉鸡鸡毒支原体的分离鉴定及最小抑菌浓度测定

doi:10.16431/j.cnki.1671-7236.2021.04.038

Abstract

Abstract: To understand the sensitivity of Mycoplasma gallisepticum (MG) to commonly used clinical drugs in Fujian White-feathered broilers,in this study,40 chicken throat swabs suspected of suffering from chronic respiratory disease (CRD) were collected from two commercial White-feathered broiler farms and inoculated on appropriate mycoplasma medium for purification.Through the analysis of the biological characteristics of the strains,bacterial morphology observation,PCR sequencing and animal regression test for identification,and the use of minimum inhibitory concentration (MIC) to determine the sensitivity of isolates to commonly used clinical antibacterial drugs.The results showed that two MG strains,named MX and HY,were successfully isolated and identified.Inoculating the isolated strain in the liquid medium of mycoplasma containing phenol red could decompose glucose to produce acid and change the color of the medium from red to yellow.A typical "poached egg" colony was observed in solid culture based on the observation of low power microscope.The colony center of the isolate after Dienes staining was dense dark blue,the edge was light blue,and did not fade after 30 minutes,and had the ability to adsorb red blood cells,with typical MG culture characteristics.The results of animal regression test and pvpA gene sequencing analysis showed that the two MG isolates had strong pathogenicity,which confirmed that they had the closest genetic relationship with R strain.The results of drug sensitivity test showed that MX and HY were highly sensitive to tiamulin,florfenicol and telithromycin,followed by enofloxacin hydrochloride and spectinomycin hydrochloride,but poor to tilmicosin,a special antibiotic for livestock and poultry with similar molecular structure and antibacterial spectrum.This difference might be related to the long-term clinical use of the drugs.The results showed that there was an epidemic of pathogenic MG in commercial White-feathered broilers in Fujian,and tiamulin could be used as the first choice for clinical prevention and treatment of MG.

Key words: Mycoplasma gallisepticum (MG); isolation and identification; biological properties; minimal inhibitory concentration (MIC)

CLC Number:

S858.31

WU Chunlin, HUANG Baoqin, LAN Tianyun, LIN Lin, ZHANG Ligen, LI Jian, LUO Zhongbao, WU Yijian. Isolation,Identification and Minimum Inhibitory Concentration Determination of Mycoplasma gallisepticum from White-feathered Broilers in Fujian[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1489-1497.

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Isolation,Identification and Minimum Inhibitory Concentration Determination of Mycoplasma gallisepticum from White-feathered Broilers in Fujian

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