RPA检测技术在禽源病原检测中的研究进展

doi:10.16431/j.cnki.1671-7236.2024.02.005

Abstract

Abstract: Recombinant polymerase amplification (RPA) is a new type of constant temperature nucleic acid amplification technology that has attracted more attention from researchers in recent years.Due to its convenient operation, fast amplification speed, high specificity, and high sensitivity, without the need for precision instruments, and being able to complete nucleic acid detection in a constant temperature environment (25-45 ℃), it is highly likely to replace traditional PCR detection technology.Since the development of RPA for over a decade, it has been widely applied in the fields of bacteria, viruses, fungi, parasites, and drug resistance.This method has been recognized by researchers as the most promising rapid molecular diagnostic tool currently, it has a wide range of application prospects.The author summarized the RPA detection, technological advantages, common detection methods, and research progress in the detection of avian pathogens, aiming to provide theoretical reference for the research of new detection technologies for clinical avian pathogens.

Key words: recombinant polymerase amplification (RPA); avian pathogens; constant temperature nucleic acid amplification technology

CLC Number:

S858.3

BAO Taotao, YANG Xianfu, LIAO Fei, WU Tongkui, MO Xinghu, WANG Zhongrong. Research Progress of RPA Detection Technology in the Detection of Avian Pathogens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 482-490.

References

[1] PIEPENBURG O,WILLIAMS C H,STEMPLE D L,et al.DNA detection using recombination proteins[J].PLoS Biology,2006,4(7):e204.
[2] 孙魁,邢微微,徐东刚.重组酶聚合酶扩增技术的研究进展[J].军事医学,2015,39(10):802-804. SUN K,XING W W,XU D G.Research progress on recombinant polymerase amplification technology[J].Military Medicine,2015,39(10):802-804.(in Chinese)
[3] 郭正洋,刘钟栋,刘小青,等.重组酶聚合酶扩增技术的研究进展[J].食品科技,2018,43(9):55-59. GUO Z Y,LIU Z D,LIU X Q,et al. Research progress on recombinant polymerase amplification technology[J].Food Science and Technology,2018,43(9):55-59.(in Chinese)
[4] 任航.三种重要传染病病原体重组酶扩增检测技术的建立与应用[D].北京:中国人民解放军军事医学科学院,2016. REN H.Establishment and application of enzyme amplification detection techniques for three important infectious disease pathogens[D].Beijing:Chinese People's Liberation Army Academy of Military Medical Sciences,2016.(in Chinese)
[5] 施奕,徐昌平,余蓓蓓,等.重组酶聚合酶扩增技术研究进展[J].病毒学报,2020,36(3):522-532. SHI Y,XU C P,YU B B,et al.Research progress on recombinant polymerase amplification technology[J].Journal of Virology,2020,36(3):522-532.(in Chinese)
[6] 王帅,杨艳歌,吴占文,等.重组酶聚合酶扩增、重组酶介导等温扩增及酶促重组等温扩增技术在食源性致病菌快速检测中的研究进展[J].食品科学,2023,44(9):297-305. WANG S,YANG Y G,WU Z W,et al.Research progress in rapid detection of foodborne pathogens using recombinant polymerase amplification,recombinant enzyme mediated isothermal amplification,and enzymatic recombinant isothermal amplification techniques[J].Food Science, 2023,44(9):297-305.(in Chinese)
[7] LOBATO I M,O'SULLIVAN C K.Recombinase polymerase amplification:Basics,applications and recent advances[J].TrAC-Trends in Analytical Chemistry,2018,98:19-35.
[8] LI J,MACDONALD J,VON STETTEN F.Correction:Review:A comprehensive summary of a decade development of the recombinase polymerase amplification[J].The Analyst, 2020,145(5):1950-1960.
[9] TWISTDX LIMITED.TwistAmp^® liquid combined instruction manual[EB/OL].https://www.twistdx.co.uk/support/rpa-assay-design/.2018.2023-08-20.
[10] SILVA G,BÖMER M,NKERE C,et al.Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification[J].Journal of Virological Methods,2015,222:138-144.
[11] TAN M,LIAO C,LIANG L,et al.Recent advances in recombinase polymerase amplification:Principle,advantages,disadvantages and applications[J].Frontiers in Cellular and Infection Microbiology,2022,12:1019071.
[12] CHENG J,KANG X,ZHANG S,et al.SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia[J].Cell,2007,131(3):584-595.
[13] XIA X,YU Y,WEIDMANN M,et al.Rapid detection of shrimp White spot syndrome virus by Real time,isothermal recombinase polymerase amplification assay[J].PLoS One,2014,9(8):e104667.
[14] ROHRMAN B A,RICHARDS-KORTUM R R.A paper and plastic device for performing recombinase polymerase amplification of HIV DNA[J].Lab on A Chip,2012,12(17):3082-3088.
[15] JAROENRAM W,OWENS L.Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome[J].Journal of Virological Methods,2014,208:144-151.
[16] KIM J Y,LEE J L.Rapid detection of Salmonella enterica serovar Enteritidis from eggs and chicken meat by Real-time recombinase polymerase amplification in comparison with the two-step Real-time PCR[J].Journal of Food Safety,2016,36(3):402-411.
[17] LIU H,ZANG Y,DU X,et al.Development of an isothermal amplification-based assay for the rapid visual detection of Salmonella bacteria[J].Journal of Dairy Science,2017,100(9):7016-7025.
[18] GENG Y,LIU G,LIU L,et al.Real-time recombinase polymerase amplification assay for the rapid and sensitive detection of Campylobacter jejuni in food samples[J].Journal of Microbiological Methods,2019,157:31-36.
[19] 许笑,王艳华,MUMAR Z K,等.鸡源产气荚膜梭菌重组酶聚合酶扩增检测方法的建立[J].中国兽医科学,2019,49(12):1475-1483. XU X,WANG Y H,MUMAR Z K,et al.Establishment of a recombinant enzyme polymerase amplification detection method for Clostridium perfringens from chicken[J].Chinese Journal of Veterinary Science,2019,49(12):1475-1483.(in Chinese)
[20] 李晓明.禽流感病毒/鸭疫里默氏杆菌RPA-LFD检测方法的建立与初步应用[D].广州:华南农业大学,2019. LI X M.Establishment and preliminary application of RPA-LFD detection method for Avian influenza virus/Riemerella anatipestifer[D].Guangzhou:South China Agricultural University,2019.(in Chinese)
[21] 王金凤,刘立兵,耿云云,等.单核细胞增生李斯特氏菌实时荧光RPA检测方法的建立及应用[J].现代食品科技,2018,34(8):213-218. WANG J F,LIU L B,GENG Y Y,et al.Establishment and application of Real-time fluorescence RPA detection method for Listeria monocytogenes[J].Modern Food Technology,2018,34(8):213-218.(in Chinese)
[22] YEHIA N,ARAFA A S,ABD EI WAHED A,et al.Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1HA gene detection[J].Journal of Virological Methods,2015,223:45-49.
[23] MA S,LI X,PENG B,et al.Rapid detection of Avian influenza A virus (H7N9) by lateral flow dipstick recombinase polymerase amplification[J].Biological&Pharmaceutical Bulletin,2018,41(12):1804-1808.
[24] TSAI S K,CHEN C C,LIN H J,et al.Combination of multiplex reverse transcription recombinase polymerase amplification assay and capillary electrophoresis provides high sensitive and high-throughput simultaneous detection of Avian influenza virus subtypes[J].Journal of Veterinary Science,2020,21(2):e24.
[25] WANG Z,YANG P P,ZHANG Y H,et al.Development of a reverse transcription recombinase polymerase amplification combined with lateral-flow dipstick assay for avian influenza H9N2HA gene detection[J].Transboundary &Emerging Diseases,2018,66(1):546-551.
[26] EL-THOLOTH M,BRANAVAN M,NAVEENATHAYALAN A,et al.Recombinase polymerase amplification-nucleic acid lateral flow immunoassays for Newcastle disease virus and infectious Bronchitis virus detection[J].Molecular Biology Reports,2019,46(6):6391-6397.
[27] FAN J,CHEN W,ZHANG Y,et al.Development of a reverse-transcription recombinase polymerase amplification assay with a lateral flow assay for rapid detection of Avian orthoavulavirus 1[J].Journal of Veterinary Diagnostic Investigation,2021,33(2):308-312.
[28] 唐豪,赵子惠,张登基,等.重组酶聚合酶扩增技术结合胶体金试纸条检测新城疫病毒方法的建立及应用[J].中国兽医科学,2023,53(3):298-303. TANG H,ZHAO Z H,ZHANG D J,et al.Establishment and application of recombinant polymerase amplification technology combined with colloidal gold test strips for detecting Newcastle disease virus[J].Chinese Journal of Veterinary Science, 2023,53(3):298-303.(in Chinese)
[29] ZHU Y,ZENG F,SUN J,et al.Application of recombinase polymerase amplification method for rapid detection of infectious Laryngotracheitis virus[J].Molecular and Cellular Probes,2020,54:101646.
[30] EL-THOLOTH M,ANIS E,BAU H H.Two stage,nested isothermal amplification in a single tube[J].The Analyst, 2021,146(4):1311-1319.
[31] ZENG F,WU M,MA L,et al.Rapid and sensitive Real-time recombinase polymerase amplification for detection of Marek's disease virus[J].Molecular and Cellular Probes,2019,48:101468.
[32] 万文妍,刘延珂,吴艳阳,等.禽腺病毒血清4型RPA-LFD快速检测方法的建立[J].中国兽医学报,2019,39(1):41-45. WAN W Y,LIU Y K,WU Y Y,et al.Establishment of a rapid detection method for Avian adenovirus serotype 4 RPA-LFD[J].Chinese Journal of Veterinary Medicine,2019,39(1):41-45.(in Chinese)
[33] ZHANG J,LIU J,AN D,et al.A novel recombinase polymerase amplification assay for rapid detection of epidemic Fowl adenovirus[J].Poultry Science,2020,99(12):6446-6453.
[34] ZHENG S Y,MA L L,WANG X L,et al.RPA-Cas12aDS:A visual and fast molecular diagnostics platform based on RPA-CRISPR-Cas12a method for infectious Bursal disease virus detection[J].Journal of Virological Methods,2022,304:114523.
[35] QU G,LI Y,ZHAO Z,et al.Establishment and application of a Real-time recombinase polymerase amplification assay for the detection of Avian leukosis virus subgroup[J].Frontiers in Veterinary Science,2022,9:847194.
[36] MA L,SHI H,ZHANG M,et al.Establishment of a Real-time recombinase polymerase amplification assay for the detection of Avian reoviru[J].Frontiers in Veterinary Science,2020,7:551350.
[37] WANG W,ZHANG Y,HUANG Y,et al.Rapid and visual detection of the emerging novel Duck reovirus by using a specific and sensitive reverse transcription recombinase polymerase amplification method[J].Journal of Virological Methods,2021,291:114098.
[38] YIN D,YIN L,WANG J,et al.Visual detection of Duck tembusu virus with CRISPR/Cas13:A sensitive and specific point-of-care detection[J].Frontiers in Cellular and Infection Microbiology,2022,12:848365.
[39] HE D,LIU G,YANG J,et al.Specific high-sensitivity enzymatic molecular detection system termed RPA-based CRISPR-cas13a for Duck tembusu virus diagnostics[J].Bioconjugate Chemistry,2022,33(6):1232-1240.
[40] LIU W J,YANG Y T,DU S M,et al.Rapid and sensitive detection of Goose parvovirus and duck-origin novel Goose parvovirus by recombinase polymerase amplification combined with a vertical flow visualization strip[J].Journal of Virological Methods,2019,266:34-40.
[41] CHENG P,WU Y,GUO S,et al.RPA assay coupled with CRISPR/Cas12a system for the detection of seven eimeria species in chicken fecal samples[J].Veterinary Parasitology,2022,311:109810.
[42] PANG Y,CONG F,ZHANG X,et al.A recombinase polymerase amplification-based assay for rapid detection of Chlamydia psittaci[J].Poultry Science,2020,100(2):585-591.
[43] 费媛媛,李兆才,娄忠子,等.动物衣原体RPA检测方法的建立[J].中国兽医科学,2018,48(2):142-147. FEI Y Y,LI Z C,LOU Z Z,et al.Establishment of an RPA detection method for animal chlamydia[J].Chinese Journal of Veterinary Sciences,2018,48(2):142-147.(in Chinese)
[44] TAN M,LIAO C,LIANG L,et al.Recent advances in recombinase polymerase amplification:Principle,advantages,disadvantages and applications[J]. Frontiers in Cellular and Infection Microbiology, 2022,12:1019071.

Research Progress of RPA Detection Technology in the Detection of Avian Pathogens

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