China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (8): 3373-3383.doi: 10.16431/j.cnki.1671-7236.2023.08.035

• Basic Veterinary Medicine • Previous Articles    

Study on the Regulation of Compound Traditional Chinese Medicine on Inflammation-proliferation of Lung in Broilers with Ascites Syndrome

CUI Liang1, WANG Keyao1, ZHANG Xinping2, WANG Huimin1, HAN Yufeng1, ZHANG Juan3, DENG Ruiqiang1, KANG Jie1, DUAN Zhibian1   

  1. 1. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    2. Shanxi Coal Center Hospital, Taiyuan 030000, China;
    3. Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2023-02-24 Published:2023-07-27

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Abstract: 【Objective】 Based on the theory of hypoxia-inflammation-endothelial proliferation,the pathogenesis of lung in broilers with ascites syndrome was explored,and the preventive and therapeutic mechanism of compound traditional Chinese medicine was studied.【Method】One hundred and fifty 8-day-old healthy AA broilers were randomly divided into 5 groups and fed routinely in blank control group.The ascites syndrome model group was established by multi-factor method of low temperature,high energy feed and high sodium drinking water.On the basis of model group,2.0,1.0 and 0.5 g/kg of compound traditional Chinese medicine were added to the daily drinking water of high,medium and low dose groups according to their body weight,respectively.The changes of clinical symptoms and anatomy were observed,the white blood cell count was detected by automatic hematology analyzer,the expression of hypoxia-inducible factor-1α(HIF-1α),vascular cell adhesion molecule 1(VCAM1),Sprouty-related,EVH1 domain-containing protein 1(SPRED1) and phosphatidylinositol 3-kinase regulatory subunit 2(PIK3R2) genes and miRNA-126-3p in lung were detected by Real-time quantitative PCR,and the expression of VCAM1,SPRED1 and PIK3R2 proteins in lung was detected by ELISA.【Result】 Compared with blank control group,broilers in model group were apathetic,had an enlarged abdomen,and on necropsy,they were found to have a large number of ascites in the abdominal cavity,pulmonary and hepatomegaly congestion,and had pericardial effusion.The white blood cell counts of broilers in model group at the age of 25,35,45 and 55 days were extremely significantly increased (P＜0.01);The expression of HIF-1α gene in lung of broilers in model group at the age of 35 days was extremely significantly increased (P＜0.01);The gene and protein expression of VCAM1 in lung of broilers in model group at the age of 15,25,35 and 45 days were extremely significantly increased (P＜0.01).The expression of SPRED1 gene and PIK3R2 protein in lung of broilers in model group at the age of 15 days were extremely significantly decreased (P＜0.01).The gene and protein expression of SPRED1 and PIK3R2 in lung of broilers in model group at the age of 25,35 and 45 days were extremely significantly decreased (P＜0.01).The expression of miRNA-126-3p in lung of broilers in model group at the age of 35 days was extremely significantly increased (P＜0.01).Compared with model group,the above results of broilers in each dose group of compound traditional Chinese medicine were improved to various degrees,and among them,the changes of each index in high dose group of compound traditional Chinese medicine were the most obvious.【Conclusion】 Inflammation and proliferation abnormalities triggered by hypoxia in lung were involved in the development of ascites syndrome in broilers.Compound traditional Chinese medicine could effectively prevent and treat ascites syndrome in broilers,among which 2.0 g/mL compound traditional Chinese medicine had the best effect.The mechanism of action was closely related to the regulation of hypoxia,inflammation,and proliferation in lung.

Key words: ascites syndrome; compound traditional Chinese medicine; hypoxia; inflammation; proliferation

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