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Effects of Cinobufagin on the Major Histocompatibility Complex ClassⅠ Molecules and Expression of their Related Genes in B16 and RMA-S Cells

ZHANG Jin1, SHI Qi-yun2, CHENG Sha2, CAO Shu-fang2, CHANG Hao2, SONG Yu1   

  1. (1. College of Animal Science, Jilin University, Changchun 130062, China; 2. College of Veterinary Medicine, Jilin University, Changchun 130062, China)
  • Revised:2014-01-16 Online:2014-04-20 Published:2014-05-27

Abstract: The study was aimed to investigate the effects of cinobufagin on the major histocompatibility complex classⅠ（MHC-Ⅰ） molecules and expression of their related genes in B16 and RMA-S cells. The effect of CBG on cell proliferation was determined by MTT assay. The expression of MHC-Ⅰ molecules was assayed by flow cytometry (FCM). The mRNA expression of LMP2, LMP7, TAP1 and TAP2 genes were deteceted by Real-time PCR. The results showed that the expression of MHC-Ⅰ was not affected significantly by CBG both in B16 and RMA-S cells（P>0.05）, while the mRNA expression of LMP2, LMP7, TAP1, TAP2 genes were increased remarkably by CBG of high concentrations. CBG may have the potential to enhance the immunogenicity of tumor by means of increasing the expression of LMP2, LMP7, TAP1 and TAP2 genes.

Key words: cinobufagin; major histocompatibility complex classⅠ molecules; immunogenicity of tumor