Abstract: This study was to obtain lipopolysaccharide (LPS) from Salmonella paratyphi C and analyze its activity. In this study, LPS was extracted from Salmonella paratyphi C by the hot phenol-water method,and purified with DNaseⅠ, RNase A and proteinase K. The contents of the purified polysaccharose, protein and nucleic acid of the purified LPS were detected, its bioactivity was detected with tachypleus amebocyte lysate (TAL). The results showed that the productivity of the purified LPS was 1.48%, the proportions of the average polysaccharose, protein and nucleic acid were 3.84%, 1.49% and 5.45%. The sequence of nucleic acid was lower than 100 bp. SDS-PAGE and silver stain showed that strip size was approximately 10 to 15 ku, the agglutinate activity of TAL was 10.99 ng/mL. The chromogenic limulus amobocyte lysate assay showed that the activity of LPS was 9.82×10⁵ EU/mg. In conclusion,the purified LPS of Salmonella paratyphi C in this study was with high purity and bioactivity.

Key words: lipopolysaccharide (LPS); Salmonella paratyphi C; hot phenol-water method; tachypleus amebocyte lysate (TAL); chromogenic limulus amobocyte lysate assay