猪MⅡ期卵母细胞玻璃化冷冻的研究

Abstract

Abstract: The study was designed to select right cryoprotectants and carrier for porcine matured oocytes to improve oocyte viability after vitrification and lay a good foundation for porcine oocytes continue development. Using 40% EG, 15% EG+15% DMSO+20% FBS, 15% EG+15% DMSO+20% SPS as cryoprotectants and taking GMP and cryotop as carrier to do porcine oocytes vitrification. The results showed that VS3 including SPS made better active of FDA (59.40%±4.93%) than VS1 （42.12%±4.08%） and VS2 (37.57%±1.21%）（P<0.05)；VS3 as cryoprotectants and cryotop as carrier made better active of FDA (83.33%±3.33%) than GMP (59.40%±4.93%)(P<0.05). The data demonstrated that use SPS to instead of FBS and cryotop as carrier could significantly improve active of oocyte after vitrification.

Key words: porcine; oocytes; SPS; cryotop; vitrification

QI Gui-long, REN Liang, ZHU Meng, FU Bo, MA Hong, LIU Di. Studies on MⅡ Stage Porcine Oocytes Vitrification[J]. .

References

1 兰志刚, 李卫娟, 李东江，等. 云岭山羊卵母细胞玻璃化冷冻的研究［J］. 中国畜牧兽医, 2012, 39(8): 161～164.
2 陆文昊.猪MⅡ期卵母细胞玻璃化冷冻的研究［D］.合肥：安徽农业大学，2010.
3 郑天威，傅祥伟，吴国权，等. 玻璃化冷冻对猪MⅡ卵母细胞皮质颗粒分布及其激活后胚胎发育能力的影响［J］.中国畜牧杂志，2012, 48（19）:29～32.
4 陶志云. 猪MⅡ期卵母细胞玻璃化冷冻的研究［D］. 合肥：安徽农业大学，2007.
5 黄凤玲，阳年生，石德顺，等. 不同血清对常温和冷冻保存牛体外受精胚胎的影响［J］.广西农业生物科学，2002, 21（1）：8～11.
6 Bou G, Liu L Q, Zheng Z, et al. Effect of chilling on porcine germinal vesicle stage oocytes at subcellular level［J］. Cryobiology, 2009, 59:54～58.
7 Fujihira T, Kishida R, Fukui Y. Developmental capacity of vitrified immature porcine oocytes following ICSI: Effects of cytochalasin B and cryoproteceants［J］. Cryobiology, 2004, 49(3): 286～290.
8 Gupta M K, Uhm S J, Lee H T. Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification［J］. Theriogenology, 2007, 67(2):238～248.
9 Kong L K, Lea S I, Cho S G, et al. Comparision of open pulled straw (OPS) vs glassmicropitte (GMP) vitrification in mouse blastocytes［J］. Theriogenology, 2000, 53:1817～1826.
10 Kuwayama M, Kato O. All-round vitrification method for human oocytes and emborys［J］.Assist Reprod Genet, 2000, 17: 477.
11 Kuwayama M, Cobo A, Vajta G. Vitrification of oocytes:General considerations and the use of the cryotop method［J］. Vitrification in Assiseed Reproduction, 2007, 7A:119～128.
12 Lin L, Du Y, Liu Y, et al. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes［J］. Reproductive Bio Medicine Online, 2009, 18（3）: 360～366.
13 Vajta G, Booth P J, Holm P. Successful vitrification of early stake bovine in vitro produced embryos with the open pulled straw (OPS) method［J］. Cryo Lett, 1997, 18:191～195.
14 Vajta G. Vitrification of the oocytes and embryos of domestic animals［J］.Anim Re Prod Sci, 2000, 60:357～364.

[1]	CHEN Kaiwen, ZHAO Yunjiao, WANG Lei, ZHANG Jianbo, MA Yuhong, WU Guofang. Transcriptome Analysis of Porcine Leydig Cells Under Different Oxygen Concentrations [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2185-2195.
[2]	CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.
[3]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[4]	OU Yunwen, PAN Qin, WANG Yang, DAI Junfei, REN Shaoke, ZHANG Yang, ZHANG Jie. Truncated Expression of ORF2 Gene of Porcine Parvovirus Type 6 and Preparation of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2487-2495.
[5]	LI Mingguo, LI Qingchun, CHEN Xin, LU Shihao, HE Fan, QI Mengfan, ZHANG Huapeng, REN Yujun, ZHANG Qingze, FU Binbin, XU Mengsi, AI Zikai, YAN Kun, FENG Yun, HUA Zaidong, HUANG Tao, BI Yanzhen. Effects of TET Demethylase on Porcine Oocyte Development [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1907-1917.
[6]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[7]	JIANG Haijun, WANG Dan, GUO Yu, WEI Li, HOU Lei, WANG Jing, ZHU Shanshan, LIU Jue. Fusion Expression and Immunogenicity of Recombinant Cap-flagellin Protein of Porcine Circovirus Type 3 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2044-2052.
[8]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[9]	HUO Mingkai, GUAN Feihu, QIU Runhui, WEI Chunyan, YU Haitao, ZHU Jiale, ZHANG Hui. Study on the Mechanism of DDX1 Inhibiting Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1556-1566.
[10]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.
[11]	ZHANG Guoqing, JIANG Yuhang, ZHANG Shuang, LI Letian, HAO Jiayi, CHEN Jing, ZOU Wancheng, LI Chang, LI Taiyuan, GAO Xu, JIN Ningyi. Preparation and Identification of PDCoV RBD Protein Peptide Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1575-1583.
[12]	LIU Xinyue, LI Lifang, WANG Zhenbao, ZHANG Wenliang, ZHANG Xin, ZHAO Hongtao, LI Pengyu, ZHENG Haotian, XIAO Jin, QI Peng. Adjuvants Screening and Immune Protection of Porcine Circovirus Type 2 Synthetic Peptide Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1068-1080.
[13]	ZHANG Jingxu, XU Yu, LIANG Haiying, ZENG Zhiyong, TANG Deyuan, WANG Bin, XU Songping, WAN Juan, ZHU Yang. Phylogenetic Evolution and Epitope Analysis of Pseudorabies Virus Guizhou Isolates and Vaccine Strains [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1093-1106.
[14]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[15]	MU Yijiao, YU Ruiming, ZHANG Liping, WANG Yonglu. Establishment and Application of ELISA for Detection of IgA Antibody Based on the Recombinant Full-length S2 Protein of Porcine Epidemic Diarrhea Virus Variant Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1185-1194.

Studies on MⅡ Stage Porcine Oocytes Vitrification

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments