儿茶提取物对猪流行性腹泻病毒感染幼龄仔猪结肠的保护作用

doi:10.16431/j.cnki.1671-7236.2025.03.037

Abstract

Abstract: 【Objective】 A pilot study was conducted to investigate the protective effect and mechanism of catechu extract (TCE) on the colon of young piglets infected with Porcine epidemic diarrhea virus (PEDV) using 7-day-old piglets.【Method】 Eighteen young piglets of similar body weight were randomly divided into three groups:Control group,PEDV group and TCE＋PEDV group,with six pigs in each group.The experimental period lasted for 11 days,with the initial adaptation period spanning from 0 to 3 days and the subsequent formal experimental period extending from 4 to 11 days.In the formal experimental period,piglets in control and PEDV groups were fed with artificial milk,and piglets in TCE＋PEDV group were fed with artificial milk and TCE (0.01 g/kg BW).The study involved the feeding of 3 mL of 10⁶ TCID₅₀/0.1 mL PEDV to piglets in both the PEDV and TCE＋PEDV groups on the 8th day.The same volume of DMEM was administered to piglets in control group.On the 11th day,all piglets were anesthetized and slaughtered,and colon tissues were collected to determine the effects of TCE on the colonic morphology and structure,antioxidant capacity,inflammatory factors,and ion channel related gene expression of piglets infected with PEDV.【Result】 Compared with control group,the colon tissue morphology of piglets in PEDV group was damaged,and the crypt depth was significantly increased (P＜0.05).Oral administration of TCE could effectively alleviate colonic damage in piglets caused by PEDV infection.Compared with PEDV group,the colon crypt depth of piglets in TCE＋PEDV group was significantly shallower (P＜0.05).Compared with control group,the activity of glutathione peroxidase (GSH-Px) in colon of piglets infected with PEDV was significantly increased (P＜0.05).Compared with PEDV group,the activities of GSH-Px,total superoxide dismutase (T-SOD),catalase (CAT) and myeloperoxidase (MPO) in colon of piglets in TCE＋PEDV group showed no significant changes (P>0.05).After PEDV infection,PEDV M,N and S genes expression could be detected in colon tissues of piglets,and the expression of interferon (IFN) signaling pathway related genes ISG15 and IRF7,inflammatory factor genes IL-1β,IL-8,REG3G,and ion channel related genes AQP10,APOB and MMP13 were significantly upregulated (P＜0.05),while the expression of IFN-β gene was significantly downregulated (P＜0.05).Compared with PEDV group,the expression of PEDV M,N,S genes,and ISG15, IL-1β, IL-8,REG3G,IRF7,APOB and MMP9 genes in colon of piglets in TCE＋PEDV group were significantly downregulated (P＜0.05),while the expression level of IFN-β gene was significantly up-regulated (P＜0.05).【Conclusion】 PEDV infection caused mucosal damage in colon tissue of young piglets,which could be alleviated by feeding 0.01 g/kg BW of TCE.

Key words: Porcine epidemic diarrhea virus (PEDV); catechu extract; colon; inflammatory response

CLC Number:

S852.65⁺1

LI Huanhuan, YU Chenmin, TIAN Xiaorong, LI Rui, LI Zongyun, ZHANG Yanyan, ZHAO Di, WANG Lei, HOU Yongqing, WU Tao. Protective Effect of Catechu Extract on the Colon of Young Piglets Infected with Porcine Epidemic Diarrhea Virus[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1360-1369.

References

[1] LI S S,YANG J P,ZHU Z X,et al.Porcine epidemic diarrhea virus and the host innate immune response[J].Pathogens,2020,9(5):367.
[2] YUTO S,AYAKO M,KOHTARO M,et al.Systemic and intestinal Porcine epidemic diarrhea virus-specific antibody response and distribution of antibody-secreting cells in experimentally infected conventional pigs[J].Veterinary Research,2021,52(1):2.
[3] PARK J E,SHIN H J.Porcine epidemic diarrhea virus infects and replicates in porcine alveolar macrophages[J].Virus Research,2014,191:143-152.
[4] SUZUKI T,SHIBAHARA T,YAMAGUCHI R,et al.Pig epidemic diarrhea virus S gene variant with a large deletion non-lethal to colostrum-deprived newborn piglets[J]. The Journal of General Virology, 2016,97(8):1823-1828.
[5] WANG D,FANG L,XIAO S.Porcine epidemic diarrhea in China[J].Virus Research,2016,226:7-13.
[6] SHUBHADEEP R,ASHOK A,GURPRIYA V,et al.Potential role of green tea catechins in the management of oxidative stress-associated infertility[J]. Reproductive BioMedicine Online,2017,34(5):487-498.
[7] MA Y,DING S J,FEI Y Q,et al.Antimicrobial activity of anthocyanins and catechins against foodborne pathogens Escherichia coli and Salmonella[J].Food Control,2019,106:106712.
[8] HUAN C C,XU W Y,NI B,et al.Epigallocatechin-3-gallate,the main polyphenol in green tea,inhibits Porcine epidemic diarrhea virus in vitro[J].Frontiers in Pharmacology, 2021,12(22):628526.
[9] FU W J,STROMBERG A J,VIELE K,et al.Statistics and bioinformatics in nutritional sciences:Analysis of complex data in the era of systems biology[J].The Journal of Nutritional Biochemistry,2010,21(7):561-572.
[10] 王思甜,徐朋涛,郑丽云,等.单宁酸对感染猪流行性腹泻病毒仔猪生长性能、肠道屏障功能和抗氧化能力的影响[J].动物营养学报,2023,35(7):4276-4286. WANG S T,XU P T,ZHENG L Y,et al.Effects of tannic acid on growth performance,intestinal barrier function and antioxidant capacity of piglets infected with Porcine epidemic diarrhea virus[J].Chinese Journal of Animal Nutrition,2023,35(7):4276-4286.(in Chinese)
[11] 惠华英,李丹丹,张雪,等.四磨汤口服液对脾虚便秘小鼠肠道黏膜厚度、隐窝深度和绒毛高度的影响[J].中国微生态学杂志,2018,30(1):10-13. HUI H Y,LI D D,ZHANG X,et al.Effects of Simo decoction on intestinal mucosal thickness,crypt depth and villus height in mice with spleen-deficiency constipation[J].Chinese Journal of Microecology,2018,30(1):10-13.(in Chinese)
[12] 谌梦,胡锦超,田晓榕,等.葛根素与茯苓多糖复合物对PEDV感染幼龄仔猪结肠的保护作用[J].饲料工业,2024，45(16)：47-54. SHEN M,HU J C,TIAN X R,et al.The protective effect of the complex of puerarin and Poria cocos polysaccharide on the colon of piglets infected with PEDV[J]. Feed Industry,2024,45(16)：47-54.(in Chinese)
[13] 张小旭,张有智,马育硕,等.多发性骨髓瘤CRAB症状与抗氧化酶活性的相关性分析[J].中国实验血液学杂志,2024,32(2):493-498. ZHANG X X,ZHANG Y Z,MA Y S,et al.Correlation between CRAB symptoms and antioxidant enzyme activity in patients with multiple myeloma[J]. Journal of Experimental Hematology,2024,32(2) 493-498.(in Chinese)
[14] SEYMOUR J K.Myeloperoxidase[J].Proceedings of the Association of American Physicians,1999,111(5):383-389.
[15] MARTINA P,LOUISA V A,IRADA K,et al.Hypochlorous acid inactivates myeloperoxidase inside phagocytosing neutrophils[J].Redox Biochemistry and Chemistry,2023,5-6:100008.
[16] 王位,朱沛,薛晓岩，等.猪流行性腹泻病毒结构蛋白及其在侵染宿主细胞中的作用[J].云南畜牧兽医,2023，1:18-21. WANG W,ZHU P,XUE X Y,et al.Structural proteins of Porcine epidemic diarrhea virus and their roles in infecting host cells[J].Yunnan Journal of Animal Science and Veterinary Medicine,2023，1:18-21.(in Chinese)
[17] LIN F,ZHANG H Y,LI L Q,et al.PEDV:Insights and advances into types,function,structure,and receptor recognition[J].Viruses,2022,14(8):1744.
[18] WANG R Y,YU R S,CHEN B Q,et al.Identification of host cell proteins that interact with the M protein of Porcine epidemic diarrhea virus[J].Veterinary Microbiology,2020,246:108729.
[19] ZHAO Y X,FAN B C,XUE J B,et al.Characterization and epitope mapping of monoclonal antibodies against PEDV N protein[J].Virology,2023,579:29-37.
[20] BRENDAN T F,FLORINE EM S,ÉRIC B,et al.How ISG15 combats viral infection[J].Virus Research,2020,286:198036.
[21] 刘莹.牛副流感病毒3型C和V蛋白通过负调控IRF7拮抗Ⅰ型干扰素表达的研究[D].哈尔滨：东北农业大学,2018. LIU Y.Study on the Bovine parainfluenza virus type 3 C and V protein antagonism against type Ⅰ interferon producting mediated by IRF7[D].Harbin:Northeast Agricultural University,2018.(in Chinese)
[22] 刘莉莉,边缘,吴圣龙,等.干扰素刺激基因ISG15对猪流行性腹泻病毒复制的作用及机制分析[J].中国畜牧兽医,2024,51(6):2545-2555. LIU L L,BIAN Y,WU S L,et al.Effect and mechanism of interferon stimulated gene ISG15 on Porcine epidemic diarrhea virus replication[J].China Animal Husbandry & Veterinary Medicine, 2024,51(6):2545-2555.(in Chinese)
[23] 罗琼玉,李文全,陈磊,等.中药灌肠对宫颈癌急性放射性直肠炎患者临床疗效及血清IL-1β、IL-8、TNF-α水平的影响[J].现代生物医学进展,2024,24(4):788-791. LUO Q Y,LI W Q,CHEN L,et al.Effect of traditional Chinese medicine enema on clinical efficacy and serum IL-1β,IL-8 and TNF-α levels in patients with acute radiation proctitis of cervical cancer[J].Modern Biomedical Advances,2024,24(4):788-791.(in Chinese)
[24] NARUKE A,NAKANO R,NUNOMURA J,et al.Tpl2 contributes to IL-1β-induced IL-8 expression via ERK1/2 activation in canine dermal fibroblasts[J].PLoS One,2021,16(11):e0259489.
[25] ANTON S,ANGELOS H.IL-8(CXCL8) correlations with psychoneuroimmunological processes and neuropsychiatric conditions[J]. Journal of Personalized Medicine,2024,14(5):488.
[26] DEBOPRIYA C,DENISE M C,IAIN A M,et al.Immune cell-intrinsic Ah receptor facilitates the expression of antimicrobial REG3G in the small intestine[J].FASEB Journal,2024,38(4):e23471.
[27] LI H X,WU M J,LI Z H,et al.Effect of supplementation with yeast polysaccharides on intestinal function in piglets infected with Porcine epidemic diarrhea virus[J].Frontiers in Microbiology, 2024,15:1378070.
[28] WANG W J,ZHANG Y,JIN W,et al.Catechin weakens diabetic retinopathy by inhibiting the expression of NF-κB signaling pathway-mediated inflammatory factors[J].Annals of Clinical and Laboratory Science,2018,48(5):594-600.
[29] 于文静.胆汁淤积下水通道蛋白AQP10下调的功能及分子机制研究[D].济南：山东大学,2019. YU W J.The study on the function and molecular mechanism of down-regulation of aquaporin 10 in cholestasis[D].Jinan:Shandong University,2019.(in Chinese)
[30] 郭伟灵,石菲菲,李路,等.灵芝提取物对高脂膳食大鼠脂质代谢及粪便菌群的影响[J].食品工业科技,2019,40(7):263-268. GUO W L,SHI F F,LI L,et al.Effects of Ganoderma lucidum extract on lipid metabolism and gut microbiota in high-fat diet fed rats[J]. Science and Technology of Food Industry,2019,40(7):263-268.(in Chinese)
[31] YEE W L,WANG Q,AGDINAOAY T,et al.Green tea catechins decrease apolipoprotein B-100 secretion from HepG2 cells[J].Molecular and Cellular Biochemistry, 2002,229(1):85-92.
[32] LEENA S S,JAYASRI K R,KAILASH K G M,et al.MMP-matrix metalloproteinase[J].Biomedical and Pharmacology Journal,2016,9(2):885-888.
[33] MORI S,PAWANKAR R,OZU C,et al.Expression and roles of MMP-2,MMP-9,MMP-13,TIMP-1,and TIMP-2 in allergic nasal mucosa[J].Allergy Asthma & Immunology Research,2012,4(4):231-239.
[34] SUHAIL M,REHAN M,TARIQUE M,et al.Targeting a transcription factor NF-κB by green tea catechins using in silico and in vitro studies in pancreatic cancer[J].Frontiers in Nutrition, 2023,9:1078642.

Protective Effect of Catechu Extract on the Colon of Young Piglets Infected with Porcine Epidemic Diarrhea Virus

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