大麻二酚对脂多糖诱导的小鼠乳腺上皮细胞炎症反应和凋亡的抑制作用

doi:10.16431/j.cnki.1671-7236.2025.01.006

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of cannabidiol (CBD) on lipopolysaccharide (LPS)-induced inflammatory response and apoptosis of mouse mammary epithelial cells (HC11). 【Method】 HC11 cells were divided into control group,LPS group and different concentrations of CBD＋LPS groups (CBD concentration was 2,4 and 6 μmol/L,respectively).The cells in control group were only treated with culture medium,and the cells in LPS group were treated with LPS.CBD＋LPS groups were pretreated with different concentrations of CBD for 1 h and then LPS was added.Cell samples were collected after 24 h of culture.The contents of inflammatory factors in the cells were detected by ELISA.Real-time quantitative PCR and Western blotting were used to detect the mRNA and protein expression levels of inflammatory cytokines,and genes related to nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways,respectively.Apoptosis rate and mitochondrial membrane potential were detected by AnnexinV-FITC/PI and JC-1 staining,respectively. 【Result】 Compared with control group,the contents and mRNA expression levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and IL-6 in LPS group were extremely significantly or significantly increased (P＜0.01 or P＜0.05),the mRNA expression and protein phosphorylation levels of NF-κB p65,nuclear factor-κB inhibitory protein α (IκBα),extracellular signal-regulated kinase (ERK),mitogen-activated protein kinase (p38),and stress-activated protein kinase (JNK) were extremely significantly or significantly increased (P＜0.01 or P＜0.05).Compared with LPS group,the contents and mRNA expression of TNF-α,IL-1β and IL-6 in cells treated with different concentrations of CBD and LPS were extremely significantly or significantly decreased (P＜0.01 or P＜0.05). The mRNA expression and protein phosphorylation levels of NF-κB p65,IκBα,ERK,p38 and JNK were extremely significantly or significantly decreased (P＜0.01 or P＜0.05).The results of apoptosis rate and mitochondrial membrane potential detection showed that,compared with control group,the apoptosis rate of HC11 cells in LPS group was significantly increased (P＜0.05),and the mitochondrial membrane potential was significantly decreased (P＜0.05). Compared with LPS group,the apoptosis rate of HC11 cells was significantly decreased after 4 μmol/L CBD and LPS co-treatment (P＜0.05),and the mitochondrial membrane potential was significantly increased (P＜0.05). 【Conclusion】 In the LPS-induced inflammation and apoptosis model of HC11 cells,the appropriate concentration of CBD could inhibit the activation of NF-κB and MAPK signaling pathways,thereby inhibiting the release of inflammatory factors to alleviate inflammation.Furthermore,it could reduce the apoptosis rate and up-regulate the mitochondrial membrane potential of HC11 cells induced by LPS,thus inhibiting the cell apoptosis.

Key words: cannabidiol; lipopolysaccharide; mouse mammary epithelial cells; inflammatory response; apoptosis

CLC Number:

S853.74

CHANG Xinxin, HUANG Xin, FAN Gang, YANG Jiaxin, ZHANG Weiwei, YANG Qingzhu, TIAN Zhe. Inhibitory Effect of Cannabidiol on LPS-induced Inflammatory Response and Apoptosis of Mouse Mammary Epithelial Cells[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 60-69.

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Inhibitory Effect of Cannabidiol on LPS-induced Inflammatory Response and Apoptosis of Mouse Mammary Epithelial Cells

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