千金藤素对牛体外卵母细胞和早期胚胎抗凋亡能力的影响

doi:10.16431/j.cnki.1671-7236.2024.07.020

Abstract

Abstract: 【Objective】 This study was aimed to explore the effect of cepharanthine (Ceph) on the antiapoptotic capacity of bovine oocytes and early embryos,so as to provide reference for improving the efficiency of bovine embryo production in vitro and new data for the pharmacological action of Ceph in anticancer process.【Method】 Bovine oocytes were cultured in in vitro mature medium containing different concentrations (0,10,100,500 and 1 000 μmol/L) of Ceph to observe the oocyte maturation rate and early embryo development after fertilization,the optimal concentration of Ceph was determined.The cathepsin B (CB) activity,the cell autophagy-related protein (LC3) level,the mRNA expression of apoptosis-related genes Survivin and Caspase-3,and the apoptosis rate of blastocysts in bovine oocyte were detected.【Result】 The maturation rate of bovine oocytes and the division rate of early embryo treated with 500 μmol/L Ceph were significantly higher than that of other groups (P＜0.05).The addition of Ceph significantly reduced the CB activity and LC3 level in bovine oocytes (P＜0.05),significantly increased the mRNA expression of Survivin gene (P＜0.05),and significantly reduced the mRNA expression of Caspase-3 gene and the apoptosis rate of blastocysts (P＜0.05).【Conclusion】 The addition of Ceph during in vitro embryo production could regulate the expression of apoptosis-related genes Survivin and Caspase-3 to affect the levels of CB and LC3,and enhance the anti-apoptotic capacity of bovine oocytes and in vitro embryo production efficiency.

Key words: cepharanthine; cattle; oocyte; early embryo; anti-apoptosis

CLC Number:

S814.8

ZHENG Xiangmin, WANG Yuqi, XU Bingjie, SUN Zhaoyang, GAO Qingshan, FANG Nanzhu, JIN Qingguo. Effect of Cepharanthine on Anti-apoptotic Ability of Bovine Oocytes and Early Embryos in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2943-2952.

References

[1] 高薇,侯微,李伟,等.细胞凋亡机制研究进展[J].中国畜牧兽医,2014,41(10):150-156. GAO W,HOU W,LI W,et al.Research progress on cell apoptosis mechanism[J].China Animal Husbandry&Veterinary Medicine,2014,41(10):150-156.(in Chinese)
[2] ELMORE S.Apoptosis:A review of programmed cell death[J].Toxicologic Pathology,2007,35(4):495-516.
[3] MICHALLET M C,SALTEL F,PREVILLE X,et al.Cathepsin-B-dependent apoptosis triggered by antithymocyte globulins:A novel mechanism of T-cell depletion[J].Blood,2003,102(10):3719-3126.
[4] SOTO P,SMITH L.BH4 peptide derived from Bcl-xL and Bax-inhibitor peptide suppresses apoptotic mitochondrial changes in heat stressed bovine oocytes[J].Molecular Reproduction and Development,2009,76(7):637-646.
[5] KONDAPURAM S K,RAMACHANDRAN H K,ARYA H,et al.Targeting survivin for cancer therapy:Strategies,small molecule inhibitors and vaccine based therapeutics in development[J].Life Sciences,2023,335:122260.
[6] SHINTANI T.Autophagy in health and disease:A double-edged sword[J].Science, 2004,306(5698):990-995.
[7] SCHAAF M B E,KEULERS T G,VOOIJS M A,et al.LC3/GABARAP family proteins:Autophagy-(un) related functions[J].FASEB Journal,2016,30(12):3961-3978.
[8] CHANG M H,HUANG F J,CHAN W H.Emodin induces embryonic toxicity in mouse blastocysts through apoptosis[J].Toxicology,2012,299(1):25-32.
[9] 丁宁,金庆国,金花子.哺乳动物早期胚胎凋亡因素的研究进展[J].延边大学农学学报,2017,39(2):89-93. DING N,JIN Q G,JIN H Z.The research progress on apoptotic factors in early mammal embryo development[J].Agricultural Science Journal of Yanbian University,2017,39(2):89-93.(in Chinese)
[10] HE C L,HUANG L Y,WANG K,et al.AL appraisal double benzyl isoquinoline alkaloid as SARS-CoV-2 a natural products library entry inhibitors[J].Signal Transduction and Targeted Therapy,2021,6:131.
[11] 尹杨峰,蒋磊.千金藤素通过MAT2B调节MAPK信号通路对宫颈癌细胞增殖和凋亡的影响[J].中国医药生物技术,2020,2:183-189. YIN Y F,JIANG L.Effect of cepharanthine on proliferation and apoptosis of cervical cancer cells by regulating MAPK signaling pathway through MAT2B[J].Chinese Medicinal Biotechnology,2020,2:183-189.(in Chinese)
[12] UNSON S,KONGSADEN C,WONGANAN P.Cepharanthine combined with 5-fluorouracil inhibits the growth of p53-mutant human colorectalcancer cells[J].Journal of Asian Natural Products Research,2020,22(4):370-385.
[13] GAO S,LI X,DING X,et al.Cepharanthine induces autophagy,apoptosis and cell cycle arrest in breast cancer cells[J].Cellular Physiology and Biochemistry,2017,41(4):1633-1648.
[14] 梁泽旭,杨万山,董秀哲.千金藤素通过PI3K-Akt信号通路抑制前列腺癌细胞DU145增殖、侵袭和迁移[J].中国免疫学杂志,2022,38(16):1963-1965. LIANG Z X,YANG W S,DONG X Z.Cepharanthine inhibits proliferation,invasion and migration of prostate cancer cell DU145 through PI3K-Akt signaling pathway[J].Chinese Journal of Immunology,2022,38(16):1963-1965.(in Chinese)
[15] BAILLY C.Cepharanthine:An update of its mode of action,pharmacological properties and medical applications[J].Phytomedicine,2019,62:152956.
[16] LIANG D,LI Q,DU L,et al.Pharmacological effects and clinical prospects of cepharanthine[J].Molecules,2022,27(24):8933.
[17] 郑泽叶,王果.千金藤素抗肿瘤药理作用机制研究进展[J].中国野生植物资源,2023,42(7):68-72. ZHENG Z Y,WANG G.Advancements on the study of anti-tumor pharmacological actions of cepharanthine[J].Chinese Wild Plant Resources,2023,42(7):68-72.(in Chinese)
[18] 刘文丽.人工养殖貉子携带的四种新型病毒的发现及千金藤素抗病毒活性研究[D].北京:北京化工大学,2023. LIU W L.Discovery of four novel raccoon dog viruses and study on antiviral activity of cepharanthine[D].Beijing:Beijing University of Chemical Technology,2023.(in Chinese)
[19] 孙业清.褪黑素对牛精液品质、卵母细胞体外成熟及体外发育能力影响的研究[D].兰州:甘肃农业大学,2016. SUN Y Q.Effect of melatonin on bovine semen quality,oocyte in vitro maturation and embryo development[D].Lanzhou:Gansu Agricultural University,2016.(in Chinese)
[20] 汪锋.白藜芦醇和褪黑素对卵母细胞成熟及胚胎发育的作用机制[D].北京:中国农业大学,2014. WANG F.Mechanisms of resveratrol and melationin on oocyte maturation and embryo development[D].Beijing:China Agricultural University,2014.(in Chinese)
[21] EL-RAEY M,GESHI M,SOMFAI T,et al.Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle[J].Molecular Reproduction Development,2011,78:250-262.
[22] GUTIERREZ-ANEZ J C,LUCAS-HAHN A,HADELER K G,et al.Melatonin enhances in vitro developmental competence of cumulus-oocyte complexes collected by ovum pick-up in prepubertal and adult dairy cattle[J].Theriogenology,2021,161:285-293.
[23] 涂龙霞,查道德,李蓓莉.组织蛋白酶B对凋亡诱导配体诱导胃腺癌细胞凋亡作用机理研究[J].中国预防医学杂志,2020,21(4):370-374. TU L X,ZHA D D,LI B L.Mechanisms of cathepsin B on TRAIL-induced apoptosis of gastric adenocarcinoma cells[J].Chinese Preventive Medicine,2020,21(4):370-374.(in Chinese)
[24] HEINRICH M,NEUMEYER J,JAKOB M,et al.Cathepsin D links TNF-induced acid sphingomyelinase to Bid-mediated caspase-9 and-3 activation[J].Cell Death and Differentiation,2004,11(5):550-563.
[25] 高驰丹,何琳莉,刘健,等.Cathepsin B在恶性肿瘤中的研究进展[J].临床与实验病理学杂志,2017,33(10):1116-1118. GAO C D,HE L L,LIU J.Research progress of Cathepsin B in malignant tumors[J].Chinese Journal of Clinical and Experimental Pathology,2017,33(10):1116-1118.(in Chinese)
[26] LIANG S,JIANG H,SHEN XH,et al.Inhibition of cathepsin B activity prevents deterioration in the quality of in vitro aged porcine oocytes[J].Theriogenology,2018,116:103-111.
[27] BALBOULA A,YAMANAKA K,SAKATANI M,et al.Cathepsin B activity is related to the quality of bovine cumulus oocyte complexes and its inhibition can improve their developmental competence[J].Molecular Reproduction and Development,2010,77(5):439-448.
[28] LIU Y,XIE Y,LIN Y,et al.Cepharanthine as a potential novel tumor-regional therapy in treating cutaneous melanoma:Altering the expression of Cathepsin B,tumor suppressor genes and autophagy-related proteins[J].Frontiers in Bioengineering and Biotechnology,2020,8:601969.
[29] TANG Z H,CAO W X,GUO X,et al.Identification of a novel autophagic inhibitor cepharanthine toenhance the anti-cancer property of dacomitinib in non-small cell lung cancer[J].Cancer Letters,2018,412:1-9.
[30] LAURENT S,YVES Q.Machine learning approaches for the identification of ligands of the autophagy marker LC3[J].Artificial Intelligence Chemistry,2023,Online ahead of print.
[31] RENNA F J,LOPEZ M H,MANIFAVA M,et al.Evaluating autophagy levels in two different pancreatic cellmodels using LC3 immunofluorescence[J].Journal of Visualized Experiments,2023,28:194.
[32] BORTNIK S,GORSKI S M.Clinical applications of autophagy proteins in cancer:From potential targets to biomarkers[J].International Journal of Molecular Sciences,1999,18(7):1496.
[33] WANG J,GARBUTT C,MA H,et al.Expression and role of autophagy-associated p62(SQSTM1) in multidrug resistant ovarian cancer[J].Gynecologic Oncology,2018,150:143-150.
[34] MIGANOVIC O,BRANKOVIC A,PANIN A N,et al.Cathepsin B:Asellsword of cancer progression[J].Cancer Letters,2019,449:207-214.
[35] YUAN J,SHAHM S,LEDOUX S,et al.The celegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme[J].Cell,1993,75(4):641-652.
[36] SHAKERI R,KHEIROLLAHI A,DAVOODI J.Apaf-1:Regulation and function in cell death[J].Bochimie,2017,135:111-125.
[37] JING M X,QI L,LI L S,et al.The Caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer[J].Cell Death Discovery,2020,6(1):112.
[38] DOU H,YU P Y,LIU Y Q,et al.Recent advances in caspase-3,breast cancer,and traditional Chinese medicine:A review[J].Journal of Chemotherapy,2023,Online ahead of print.
[39] DARCY M S.Cell death:A review of the major forms of apoptosis,necrosis and autophagy[J].Cell Biology International,2019,43(6):582-592.
[40] WARRIER N M,AGARWAL P,KUMAR P.Emerging importance of Survivin in stem cells and cancer:The development of new cancer therapeutics[J].Stem Cell Reviews and Reports,2020,16(5):828-852.
[41] GARG H,SURI P,GUPTA J C,et al.Survivin:A unique target for tumor therapy[J].Cancer Cell International,2016,16(1):49-63.
[42] CHEUNG C H,HUANG C C,TSAI F Y,et al.Survivin-biology and potential as a therapeutic target in oncology[J].Onco Targets and Therapy,2013,6(10):1453-1462.
[43] SHAHRIYAR S A,MIN WOO S,UN SEO S,et al.Cepharanthine enhances TRAIL-mediated apoptosis through STAMBPL1-Mediated downregulation of Survivin expression in renal carcinoma cells[J].International Journal of Molecular Sciences,2018,19(10):3280.
[44] WANG Y Q,QU H X,DONG Y W,et al.Inhibition of FSP1 impairs early embryo developmental competence in pigs[J].Theriogenology,2024,214:257-265.
[45] LOU H,LI N,GUAN Y,et al.Association between morphologic grading and implantation rate of euploid blastocyst[J].Journal of Ovarian Research,2021,14(1):18.

Effect of Cepharanthine on Anti-apoptotic Ability of Bovine Oocytes and Early Embryos in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	DING Hui, LIU Xin, WANG Ruiling, MAO Yongxia, WANG Jiandong, GUO Yansheng. Effect of Dietary Addition of Soy Isoflavones on Production and Reproductive Performance of Angus Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2871-2879.
[2]	HUANG Yuechuan, ZHANG Hailiang, XU Wei, HAN Liyun, ZHOU Jiamin, MA Liqin, WEN Wan, WANG Yachun. Genetic Parameters for Conformation Traits of Dairy Cattle in Ningxia [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2908-2922.
[3]	ZHANG Yan, WEI Zhitong, HU Yehao, ZHANG Huaju, ZHANG Man, FU Tong, LIU Xian, LI Zhigang, QI Xinglei, WANG Eryao, ZHANG Zijing, LIANG Dong, GAO Tengyun. Genetic Diversity and Population Structure Analysis of Jiaxian Red Cattle Based on Whole Genome Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2933-2942.
[4]	JIANG Lei, WANG Yuxin, WAN Yuan, ZAN Linsen, WANG Hongbao. Bioinformatics and Expression Characteristics Analysis of MGP Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2261-2272.
[5]	WANG Suwan, FENG Xiaofang, TONG Lijia, LI Desheng, WANG Yu, FENG Yuan, JIANG Qiufei, XU Jun, CHEN Yafei, GU Yaling, ZHANG Juan. Genetic Parameter Estimation of Growth and Developmental Traits in Angus Cattle During Weaning Stage [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2517-2523.
[6]	MO Xianhong, LI Junjie, GUO Cheng, WEN Zhaoyu, ZOU Yuzhu, ZHAO Wenbo, XU Zhenjun. Research Progress on Vitrification Injury of Oocytes in Livestock [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2524-2532.
[7]	WANG Wenya, XIE Xinyan, YAN Dalong, WEI Penghao, YANG Jianling, BI Run, LI Qing, MA Jun, ZOU Fengcai. Infection Status of Giardia duodenalis in Cattle in Yunnan Province Based on gdh Gene [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2644-2652.
[8]	LUO Anfeng, HUA Zaidong, YANG Caixia, CHEN Fan. Effect of Nuclear Membrane Pore Subcomplex Nup98/Rae1 on Meiotic Maturation of Mouse Oocytes in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1998-2006.
[9]	YIN Siqi, ZHAO Gangkui, GAO Haixu, SUN Honghong, XIN Yiran, ZAN Linsen, ZHAO Chunping. Cloning, Bioinformatics Analysis and Tissue Expression of FADS Gene Family in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 903-915.
[10]	LI Youwei, CHENG Yazhuo, SHANG Jiyong, ZHANG Tinglong, SUN Mingju. Research Progress on in vitro Maturation of Small Follicle-derived Oocytes in Mammalian [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1171-1182.
[11]	JIA Xuechun, RONG Yanhua, ZHU Zhiwei, LI Pengfei. Whole-Genome Resequencing Reveals Genetic Potential for Phenotypic Merit in Jinnan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 1-10.
[12]	RONG Yanhua, JIA Xuechun, LI Pengfei, TIAN Guofu, ZHU Zhiwei. Analysis of Genetic Structure Characteristics and Selection Signal in Jinnan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 160-171.
[13]	CHEN Shaokan, LUO Hanpeng, MA Yehua, LIU Gang, WANG Di, HAO Kebi, WANG Yachun, GUO Gang. Selection and Influencing Factors Analysis of Key Milking Flow Rate Indicators of Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 212-219.
[14]	WANG Yaqin, ZONG Junlin, ZHANG Tao, GUAN Weijun. Isolation,Culture and Differentiation Potential of Bovine Adipose Mesenchymal Stem Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3530-3540.
[15]	LIU Keke, XUE Mengqi, TANG Feitai, DU Qiansheng, WANG Xinyu, DONG Yinyu, WANG Xiaoli. Effect of AFP Ⅲ on Mitochondria of Vitrified Mouse Oocytes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3180-3188.