秦川牛FADS基因家族克隆、生物信息学分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2024.03.002

Abstract

Abstract: 【Objective】 The purpose of this experiment was to systematically analyze the fatty acid desaturases (FADS) gene family in Qinchuan cattle,clarify the spatiotemporal expression patterns of these members in various tissues and different differentiation stages of preadipocytes,and lay a foundation for further exploring the function and regulatory roles of FADS gene family in bovine fat deposition.【Method】 Different tissue samples of Qinchuan cattle were collected,the preadipocytes were isolated from perirenal fat,and the CDS region sequences of FADS gene family were amplified by PCR,and the sequence characteristics were predicted by bioinformatics software.Real-time quantitative PCR was used to detect the temporal and spatial expression profiles of FADS gene family members in different tissues of adult cattle and during the differentiation of prerenal adipocytes.【Result】 The CDS region of 3 members of FADS gene family (FADS1,FADS2 and FADS3) was amplified successfully with the size of 1 425,1 335 and 1 332 bp,respectively.The phylogenetic tree results showed that the amino acid sequences of FADS gene family members of Qinchuan cattle had the highest similarity with Bos indicus,and were far related to Mus musculus.The results of bioinformatics prediction showed that the FADS gene family were stable hydrophilic proteins,and the secondary structure was mainly composed of alpha helix,and the tertiary structure was consistent with the predicted secondary structure.FADS1 and FADS2 were mainly located in the endoplasmic reticulum (44.4% and 44.4%),and FADS3 was mainly located in the cell membrane (39.1%).Real-time quantitative PCR results showed that the expression of FADS1 and FADS3 genes were higher in muscle tissue,and the expression of FADS2 gene was the highest in liver, and significantly higher than that in other tissues (P<0.05).With the adipogenic differentiation of adipocytes,compared to other differentiation time, the expression of FADS1 and FADS2 genes were the highest on days 2 (P<0.05),while the expression of FADS3 gene was the lowest on days 2 (P<0.05).【Conclusion】 FADS1, FADS2 and FADS3 of FADS gene family in Qinchuan cattle were successfully cloned, which were highly conserved Bos indicus, Capra hircus and Ovis aries and other mammals. FADS gene family were relatively stable hydrophilic proteins, FADS1 and FADS3 genes were highly expressed in muscle, and FADS2 gene was highly expressed in liver. In the process of adipogenic differentiation of adipocytes, FADS1 and FADS2 gene expressions were the highest and FADS3 gene expression was the lowest on days 2 of differentiation. The results laid a foundation for further research on the mechanism of FADS gene family influencing fat deposition in beef cattle by regulating adipocyte differentiation.

Key words: Qinchuan cattle; FADS gene family; bioinformatics; expression

CLC Number:

S823

YIN Siqi, ZHAO Gangkui, GAO Haixu, SUN Honghong, XIN Yiran, ZAN Linsen, ZHAO Chunping. Cloning, Bioinformatics Analysis and Tissue Expression of FADS Gene Family in Qinchuan Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 903-915.

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Cloning, Bioinformatics Analysis and Tissue Expression of FADS Gene Family in Qinchuan Cattle

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