2023年华南地区猪繁殖与呼吸综合征病毒的遗传变异分析

doi:10.16431/j.cnki.1671-7236.2025.01.030

Abstract

Abstract: 【Objective】 This study was aimed to investigate the genetic variation of Porcine reproductive and respiratory syndrome virus (PRRSV) in pig farms in South China in 2023,and provide theoretical basis for PRRSV prevention and control in South China. 【Method】 Real-time quantitative PCR was used to detect PRRSV in 328 samples collected from different pig farms in South China.ORF5 gene of 29 representative PRRSV-positive samples from different pig farms were sequenced and analyzed for genetic evolution. 【Result】 The positive rate of PRRSV in all samples was 54.27% (178/328).The sequence analysis of ORF5 gene of 29 strains of PRRSV showed that all the obtained strains belonged to the genotype Ⅱ.The nucleotide sequence similarity between the 29 PRRSV ORF5 gene was 83.7% to 100%,and the amino acid sequence similarity was 83.0% to 100%.Among them,6 strains belonged to lineage 1 represented by the recombinant strain NADC30-like,2 strains belonged to lineage 5 represented by the classical strain VR2332,and 21 strains belonged to lineage 8 represented by the highly pathogenic strain JXA1.The amino acid mutations derived from the ORF5 gene were mainly concentrated in 2 highly variable regions,1 bait epitopes,and 3 transmembrane regions.5 strains of lineage 1 showed a V27A mutation in the bait epitope region,while 2 strains of lineage 5 showed a V29A mutation in the bait epitope region.In the neutralizing epitope region,8 strains of lineage 1 exhibited I39L mutations,and 5 strains of lineage 8 exhibited I39F mutations.In virulence-related loci,some strains had undergone mutations in R13Q,R151K,R151I and R151G.In addition,there were more variations in the N-glycation sites of lineage 1,mainly concentrated in amino acids at positions 30－32,32－34,33－35,34－36,35－37 and 57－59,while the N-glycation sites of lineage 5 and lineage 8 were relatively conserved. 【Conclusion】 At present,there was a high positive rate of PRRSV in pig farms in South China.The prevalence of PRRSV was characterized by multi-lineage genotype Ⅱ strains,with lineage 8 and lineage 1 strains accounting for a relatively high proportion and having complex and diverse variations.It was necessary to strengthen the monitoring of PRRSV,understand the genetic variation among PRRSV strains,and timely implement prevention and control measures.

Key words: Porcine reproductive and respiratory syndrome virus (PRRSV); genetic variation; ORF5 gene; lineage

CLC Number:

S852.65⁺1

LI Songbei, XIE Yongsheng, LONG Xiaoqin, ZHANG Xiaoxiao, CHEN Yongjie, ZHANG Chunhong, JIAO Maoxing, GUO Chunhe. Genetic Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus in South China in 2023[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 331-340.

References

[1] HAN J,ZHOU L,GE X,et al.Pathogenesis and control of the Chinese highly pathogenic Porcine reproductive and respiratory syndrome virus[J].Veterinary Microbiology,2017,209:30-47.
[2] DWIVEDI V,MANICKAM C,BINJAWADAGI B,et al.Evaluation of immune responses to Porcine reproductive and respiratory syndrome virus in pigs during early stage of infection under farm conditions[J].Virology Journal,2012,9:45.
[3] YUZHAKOV A G,RAEV S A,SHCHETININ A M,et al.Full-genome analysis and pathogenicity of a genetically distinct Russian PRRSV-1 Tyu16 strain[J].Veterinary Microbiology,2020,247:108784.
[4] ZHANG L,FENG Y,MARTIN D P,et al.Genetic diversity and phylogenetic analysis of the ORF5 gene of PRRSV from Central China[J].Research in Veterinary Science,2017,115:226-234.
[5] OPRIESSNIG T,HALBUR P G,YOON K J,et al.Comparison of molecular and biological characteristics of a modified live Porcine reproductive and respiratory syndrome virus (PRRSV) vaccine (Ingelvac PRRS MLV),the parent strain of the vaccine (ATCC VR2332),ATCC-VR2385,and two recent field isolates of PRRSV[J].Journal of Virology,2002,76(23):11837-11844.
[6] ROPP S L,WEES C E,FANG Y,et al.Characterization of emerging European-like Porcine reproductive and respiratory syndrome virus isolates in the United States[J].Journal of Virology,2004,78(7):3684-3703.
[7] LENG C,TIAN Z,ZHANG W,et al.Characterization of two newly emerged isolates of Porcine reproductive and respiratory syndrome virus from Northeast China in 2013[J].Veterinary Microbiology,2014,171(1-2):41-52.
[8] KANG H,YU J E,SHIN J,et al.Geographic distribution and molecular analysis of Porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016[J].BMC Veterinary Research,2018,14(1):160.
[9] 郭宝清,陈章水,刘文兴,等.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996,2:3-7. GUO B Q,CHEN Z S,LIU W X,et al. Isolation and identification of Porcine reproductory and respiratory syndrome (PRRS) virus from aborted fetuses suspected of PRRS[J]. Chinese Journal of Animal and Poultry Infectious Disease, 1996, 2: 3-7.(in Chinese)
[10] TIAN K,YU X,ZHAO T,et al.Emergence of fatal PRRSV variants:Unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark[J].PLoS One,2007,2(6):e526.
[11] LU W H,TUN H M,SUN B L,et al.Re-emerging of Porcine respiratory and reproductive syndrome virus (lineage 3) and increased pathogenicity after genomic recombination with vaccine variant[J].Veterinary Microbiology,2015,175(2-4):332-340.
[12] LUNNEY J K,BENFIELD D A,ROWLAND R R R.Porcine reproductive and respiratory syndrome virus:An update on an emerging and re-emerging viral disease of swine[J]. Virus Research,2010,154(1-2):1-6.
[13] XU H,SONG S,ZHAO J,et al.A potential endemic strain in China:NADC34-like Porcine reproductive and respiratory syndrome virus[J].Transboundary and Emerging Diseases,2020,67(4):1730-1738.
[14] 康溥,任照文,蔡汝健,等.广东及周边省份猪繁殖与呼吸综合征病毒2型谱系8毒株变异规律和传播研究[J].中国畜牧兽医,2023,50(5):2001-2011. KANG B,REN Z W,CAI R J,et al.Variation regularity and transmission of PRRSV-2 lineage 8 strains in Guangdong and surrounding provinces[J].China Animal Husbandry & Veterinary Medicine,2023,50(5):2001-2011.(in Chinese)
[15] ZHANG S,ZENG L,SU B,et al.The glycoprotein 5 of Porcine reproductive and respiratory syndrome virus stimulates mitochondrial ROS to facilitate viral replication[J].mBio,2023,14(6):e0265123.
[16] CHOI J C,KIM M S,CHOI H Y,et al.Porcine reproductive and respiratory syndrome virus engineered by serine substitution on the 44th amino acid of GP5 resulted in a potential vaccine candidate with the ability to produce high levels of neutralizing antibody[J].Veterinary Sciences,2023,10(3):191.
[17] SONG T,FANG L,WANG D,et al.Quantitative interactome reveals that Porcine reproductive and respiratory syndrome virus nonstructural protein 2 forms a complex with viral nucleocapsid protein and cellular vimentin[J].Journal of Proteomics,2016,142:70-81.
[18] 郭川.PRRS病毒GP5的基因序列分析及其重组蛋白的应用研究[D].厦门:厦门大学,2006. GUO C.Gene sequence analysis of PRRS virus GP5 and application research of its recombinant protein[D].Xiamen:Xiamen University,2006.(in Chinese)
[19] 邓吉平,陈指龙,郭松扬念,等.猪繁殖与呼吸综合征病毒湖南分离株ORF5基因的遗传进化分析[J].中国畜牧兽医,2021,48(10):3795-3802. DENG J P,CHEN Z L,GUO S Y N,et al.Genetic evolution analysis of ORF5 gene of Porcine reproductive and respiratory syndrome virus isolated from Hunan province[J] China Animal Husbandry & Veterinary Medicine,2021,48(10):3795-3802.(in Chinese)
[20] ZHANG Q,BAI J,HOU H,et al.A novel recombinant Porcine reproductive and respiratory syndrome virus with significant variation in cell adaption and pathogenicity[J].Veterinary Microbiology,2017,208:150-158.
[21] 蒋智勇,蔡汝健,李艳,等.2014－2016年广东省PRRSV GP5基因遗传变异分析[J].广东农业科学,2016,43(12):90-95. JIANG Z Y,CAI R J,LI Y,et al.Genetic variation analysis of GP5 gene of PRRSV in Guangdong province during 2014-2016[J].Guangdong Agricultural Science,2016,43(12):90-95.(in Chinese)
[22] AN T Q,LI J N,SU C M,et al.Molecular and cellular mechanisms for PRRSV pathogenesis and host response to infection[J].Virus Research,2020,286:197980.
[23] LUNNEY J K,FANG Y,LADINIG A,et al.Porcine reproductive and respiratory syndrome virus (PRRSV):Pathogenesis and interaction with the immune system[J].Annual Review of Animal Biosciences,2016,4:129-154.
[24] OPRIESSNIG T,MATTEI A A,KARUPPANNAN A K,et al.Future perspectives on swine viral vaccines:Where are we headed?[J]. Porcine Health Management,2021,7(1):129-154.
[25] NATHUES H,ALARCON P,RUSHTON J,et al.Modelling the economic efficiency of using different strategies to control porcine reproductive & respiratory syndrome at herd level[J].Preventive Veterinary Medicine,2018,152:89-102.
[26] OBA P,WIELAND B,MWIINE F N,et al.Status and gaps of research on respiratory disease pathogens of swine in Africa[J].Porcine Health Management,2020,6(1):5.
[27] LI S,QIU M,LI S,et al.A chimeric Porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection[J].Veterinary Research,2024,55(1):28.
[28] OSTROWSKI M,GALEOTA J A,JAR A M,et al.Identification of neutralizing and nonneutralizing epitopes in the Porcine reproductive and respiratory syndrome virus GP5 ectodomain[J].Journal of Virology,2002,76(9):4241-4250.
[29] ANSARI I H,KWON B,OSORIO F A,et al.Influence of N-linked glycosylation of Porcine reproductive and respiratory syndrome virus GP5 on virus infectivity,antigenicity,and ability to induce neutralizing antibodies[J].Journal of Virology,2006,80(8):3994-4004.
[30] ZHANG Q,XU X,YOU S,et al.Emerging of two new subgenotypes of Porcine reproductive and respiratory syndrome viruses in Southeast China[J].Microbial Pathogenesis,2016,97:27-33.
[31] KEY K F,HAQSHENAS G,GUENETTE D K,et al.Genetic variation and phylogenetic analyses of the ORF5 gene of acute Porcine reproductive and respiratory syndrome virus isolates[J].Veterinary Microbiology,2001,83(3):249-263.
[32] WEI Y,DAI G,HUANG M,et al.Construction of an infectious cloning system of Porcine reproductive and respiratory syndrome virus and identification of glycoprotein 5 as a potential determinant of virulence and pathogenicity[J].Frontiers in Microbiology,2023,14:1227485.
[33] LUO Q,ZHENG Y,ZHANG H,et al.Research progress on glycoprotein 5 of Porcine reproductive and respiratory syndrome virus[J].Animals (Basel),2023,13(5):813.
[34] PIRZADEH B,GAGNON C A,DEA S.Genomic and antigenic variations of Porcine reproductive and respiratory syndrome virus major envelope GP5 glycoprotein[J].Canadian Journal of Veterinary Research,1998,62(3):170-177.
[35] ZHOU Y,YU H,TIAN Z,et al.Genetic diversity of the ORF5 gene of Porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008[J].Virus Research,2009,144(1-2):136-144.
[36] ALLENDE R,KUTISH G F,LAEGREID W,et al.Mutations in the genome of Porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype[J].Archives of Virology,2000,145(6):1149-1161.
[37] 石青青,姜轩,张奥,等.2016－2017年我国华北地区猪繁殖与呼吸综合征病毒类NADC30毒株分子遗传演化分析[J].中国动物传染病学报,2020,28(3):80-85. SHI Q Q,JIANG X,ZHANG A,et al.Genetic variations of NADC30-like Porcine reproductive and respiratory syndrome virus from 2016 to 2017 in North China[J].Chinese Journal of Animal Infectious Diseases,2020,28(3):80-85.(in Chinese)

Genetic Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus in South China in 2023

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YU Jingxue, WEI Shanshan, QIN Shaomin, WU Jianmin, YANG Lihua, CHEN Fenglian, XU Lishi, QIN Shuying, HUA Jun, WEI Jue, FANG Fang, LIU Jinfeng. Establishment of a Rapid Detection Method for Porcine Reproductive and Respiratory Syndrome Virus Based on CRISPR/Cas12a-RT-RAA [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3237-3246.
[2]	QIAO Changhong, CHEN Jing, LUO Qin, LIU Baoling, HE Zhenwen, LIU Dingyu, CHEN Xiangyu, WANG Xiaohu, WANG Gang, BAI Aiquan, CAI Rujian. Advances in Inflammation Induced by Porcine Reproductive and Respiratory Syndrome Virus and Anti-inflammatory Drugs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3528-3539.
[3]	DONG Zexia, LIN Xin, ZHOU Qilyu, WANG Nan, HUANG Lei, LIU Zhiguo, FENG Zheng, MU Yulian. Construction and PRRSV Infection Characteristic Analysis of CD163 Gene Knockout iPAMs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3471-3483.
[4]	LUO Qin, LIU Baoling, QIAO Changhong, CHEN Xiangyu, LIU Dingyu, WANG Xiaohu, WANG Gang, LIU Hao, CAI Rujian. Research Progress on the Role of Lipid Metabolism and Glucose Metabolism in PRRSV-infected Host Cells [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1686-1695.
[5]	LIU Bo, GONG Zhiguo, HASI Surong. Effects of MLKL on Secretion of Inflammatory Mediators and Activation of Inflammatory Signaling Pathway Induced by Staphylococcus aureus [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(12): 5560-5569.
[6]	WANG Linqing, ZHAO Li, CHEN Ximeng, WU Shaofeng, HAN Yingying, LIU Fang, JIN Yue, CHEN Hongying. Serological Investigation of Porcine Pseudorabies Virus Infection and Genetic Variation Analysis of gE Gene in Henan Province [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3364-3372.
[7]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[8]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[9]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[10]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[11]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[12]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[13]	GOU Danni, WANG Gang, SHEN Xuemei, NIE Liang, HUI Wenjing, GULIPARI Aikebai, YANG Yaling, LIAO Juan, YU Shigang. Polymorphism of LIPE Gene Exon 1 and Its Association with Body Weight and Intramuscular Fat Content in Muchuan Black-bone Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4973-4982.
[14]	WANG Yulong, LI Ke, LI Dong, SHI Xin'e, YANG Gongshe, YU Taiyong. Genome Assembly Technology and Its Research Progress on Genetic Variation of Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4085-4095.
[15]	YAN Pupu, LIU Xin, LIN Bing, LIU Jiali, XIA Jinjin, HUANG Yongxi, BAI Ruonan, LIU Guoping, YANG Xiaolin, GUO Liwei. Exploring of the Infection Characteristics of Porcine Reproductive and Respiratory Syndrome Virus and the Prevention and Control Mechanism of Yinhua Gancao Decoction Based on Bioinformatics [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4196-4209.