基因组组装技术及其在畜禽遗传变异中的研究进展

doi:10.16431/j.cnki.1671-7236.2023.10.022

Abstract

Abstract: The first and second generation sequencing technology has been widely used in species genome assembly,but with the deepening of genomics research,which has been unable to meet the needs of comprehensive detection of genome variation and complete analysis of unknown regions of the genome.The third generation sequencing technology has brought genome sequencing into the era of long reading, and genome assembly based on the third generation sequencing data has become the mainstream choice for genome research of various species.High-quality genome assembly plays a crucial role in animal and plant genetics and genomics research,providing essential support for comprehensive understanding of genome structure,function,and evolution.The author reviewed the development and application of the first,second and third generation sequencing technology and auxiliary assembly technology,introduced the splicing principle,application scope and corresponding bioinformatics tools of genome assembly algorithm,and elaborated the application of high-quality reference genome in the detection of genetic variation and functional gene mining of livestock and poultry combined with the published research.In order to provide reference for the protection of animal germplasm resources and molecular breeding practice in the future.

Key words: genome sequencing technology; assisted assembly; assembly algorithm; livestock genome; genetic variation

CLC Number:

S813

WANG Yulong, LI Ke, LI Dong, SHI Xin'e, YANG Gongshe, YU Taiyong. Genome Assembly Technology and Its Research Progress on Genetic Variation of Livestock and Poultry[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4085-4095.

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Genome Assembly Technology and Its Research Progress on Genetic Variation of Livestock and Poultry

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