河南省猪伪狂犬病病毒野毒感染血清学调查及gE基因遗传变异分析

doi:10.16431/j.cnki.1671-7236.2023.08.034

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the epidemiological characteristics and genetic variation of Pseudorabies virus (PRV) in swine in Henan Province.【Method】 gE antibody levels were detected by ELISA in 25 411 serum samples collected from 766 pig farms in Henan Province during 2020-2021.gE gene was amplified by PCR in 251 samples from 47 pig farms suspected of PRV-infected pigs,and the positive samples were inoculated with ST cells for virus isolation,gE gene sequencing and genetic evolution analysis.【Result】 The total positive rate of gE antibody in serum samples was 18.42%,which decreased from 20.32% in 2020 to 16.69% in 2021.The positive rate in pig farms was 50.26%.With the increase of pig farm size, the positive rate of farm and serum sample showed a downward trend. The farm and serum positive rate in commodity farm, slaughterhouse and pig breeding farm decreased successively. The positive rates of gE antibody in eastern, western, southern, northern and central Henan were 15.05%, 23.48%, 16.50%, 16.69% and 20.10%, respectively. The positive rates were the highest from January to March and the lowest from October to December.The positive rate of PRV in tissue samples was 15.14% (38/251).A total of 9 PRV isolates were isolated from PRV positive samples.Phylogenetic analysis based on the gE complete sequences indicated that all isolates belonged to Genotype Ⅱ and there were both classical strains and variant strains in pig herds.【Conclusion】 PRV classical strains and PRV variant strains still existed in pig farms in Henan province.The results provided reference for the prevention and control and purification of PRV in Henan province.

Key words: pig; Pseudorabies virus(PRV); serological investigation; gE gene; genetic variation

CLC Number:

S852.65⁺1

WANG Linqing, ZHAO Li, CHEN Ximeng, WU Shaofeng, HAN Yingying, LIU Fang, JIN Yue, CHEN Hongying. Serological Investigation of Porcine Pseudorabies Virus Infection and Genetic Variation Analysis of gE Gene in Henan Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3364-3372.

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Serological Investigation of Porcine Pseudorabies Virus Infection and Genetic Variation Analysis of gE Gene in Henan Province

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