五指山猪和长白猪断奶仔猪肠道免疫性能及微生物多样性比较分析

doi:10.16431/j.cnki.1671-7236.2023.08.009

Abstract

Abstract: 【Objective】 To explore the difference of effects of early weaning on intestinal immunity and microbial diversity in Wuzhishan and Landrace pigs.【Method】 Six 50-day-old Landrace pigs and six Wuzhishan pigs (weaned at 28-day-old) with good growth,development and nutritional status were selected to collect the tissues,contents and mucosa of each segment of the small intestine.Paraffin section (HE staining),16S rDNA V3-V4 gene high-throughput sequencing and Real-time PCR were used to compare the differences between the two breeds in intestinal morphology,intestinal flora and the relative mRNA expression of intestinal mucosal immune-related genes.【Result】 ① The jejunal villus height of Wuzhishan pigs was significantly lower than that of Landrace pigs (P＜0.05).②The intestinal microbial diversity of Landrace pigs was more abundant in duodenum and ileum than that of Wuzhishan pigs (P＜0.05).③At the phylum level,the abundance of Firmicutes in the jejunum and ileum of Wuzhishan pigs was significantly higher than that of Landrace pigs,but Proteobacteria showed a reverse expression trend (P＜0.05).At the genus level,the abundance of Lactobacillus in ileum of Wuzhishan pigs was significantly higher than that of Landrace pigs (P＜0.05).④The detection results of mucosal immune related gene expression showed that the mRNA expression levels of anti-inflammatory factor IL-10 and tight junction protein ZO1 in duodenum of Landrace pigs were significantly higher than those of Wuzhishan pigs (P＜0.05),while the mRNA expression levels of pro-inflammatory factor TNF-α in jejunum and ileum were significantly lower than those in Wuzhishan pigs (P＜0.05).【Conclusion】 Compared with Landrace pigs,Wuzhishan pigs had poor intestinal immune performance and microbial diversity after early weaning.The weaning age of Wuzhishan pigs could be appropriately extended to reduce the diarrhea rate and ensure the survival rate of weaned piglets.

Key words: Wuzhishan pig; Landrace pig; early weaning; immunity; gut microbial diversity

CLC Number:

S828

ZHANG Ting, WEI Limin, LIU Guangliang, WANG Feng, CHAO Zhe, REN Yuwei, LIU Hailong, ZHENG Xinli, HUANG Lili, SUN Ruiping. Comparative Analysis of Intestinal Microbial Diversity and Immunity in the Small Intestine of Weaned Piglets from Wuzhishan Pigs and Landrace Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3113-3122.

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Comparative Analysis of Intestinal Microbial Diversity and Immunity in the Small Intestine of Weaned Piglets from Wuzhishan Pigs and Landrace Pigs

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