香猪CYP3A29基因3'-UTR结构变异及其与肌肉雄激素水平的关联分析

doi:10.16431/j.cnki.1671-7236.2023.08.017

Abstract

Abstract: 【Objective】 This study was conducted to investigate the effect of the structural variation (SV) in the pig-specific cytochrome P450 3A 29 (CYP3A29) gene 3'-UTR on its expression,and analyze the relationship between the SV and the formation of meat quality traits in Xiang pigs.【Method】 The repeated elements,miRNA,and RBPs binding sites in the SV of CYP3A29 gene 3'-UTR were predicted by UCSC,miRBase,PITA and RBPsuite softwares in Xiang pigs and Large White pigs.The SV site was genotyped by PCR,the genotype frequency,gene frequency and Hardy-Weinberg equilibrium were calculated.The mRNA expression of CYP3A29 gene was detected by Real-time quantitative PCR.The content of CYP3A29 protein and the level of androgen (T and DHT) in muscle were detected by ELISA.Pearson correlation analysis between the contents of T and DHT and different genotypes was performed by SPSS 17.0 software.【Result】 Bioinformatics analysis results showed that the SV length of CYP3A29 gene 3'-UTR was 303 bp,and there was a short interspersed element (SINE) with a length of 241 bp in this variation,belonging to Pre0_SStRNA family,located in Chr3:6 819 917―6 820 161,containing 33 miRNAs and 13 RBPs binding sites.The genotyping confirmed that the SV in Xiang pigs and Large White pigs showed rich polymorphism,which contained insertion (II),hybrid (ID) and deletion (DD).The frequency of D allele in Xiang pigs was higher than that in Large White pigs.The SV was in Hardy-Weinberg equilibrium in Xiang pigs (P＞0.05),but not in Large White pigs (P＜0.05).The results of Real-time quantitative PCR and ELISA showed that the mRNA expression and protein content of ID genotype of CYP3A29 gene in Xiang pigs were significantly higher than II and DD genotypes,respectively (P＜0.05).The correlation analysis results showed that the expression of CYP3A29 gene of different genotypes was significantly correlated with T concentration of muscle tissue in Xiang pigs (P＜0.05),but not correlated with DHT concentration (P＞0.05).The T concentration of ID genotype in Xiang pigs was significantly higher than that of DD and II genotypes (P＜0.05),and DHT concentration of DD genotype was significantly higher than that of ID and II genotypes (P＜0.05).【Conclusion】 The SV of 303 bp in CYP3A29 gene 3'-UTR was polymorphic in Xiang pigs,and the deletion D allele was the main one,that was the SV lacked the SINE element in Xiang pigs,which leaded to the up-regulation of CYP3A29 gene expression,thereby affecting the T concentration of muscle tissue in Xiang pigs.In summary,the SV of CYP3A29 gene 3'-UTR was correlated with the meat quality traits in Xiang pigs.

Key words: Xiang pigs; CYP3A29 gene; structural variation; androgen; meat quality

CLC Number:

S828

LIANG Xiaofen, RAN Xueqin, NIU Xi, LI Sheng, HUANG Shihui, WANG Jiafu. Association Analysis Between Structural Variation of CYP3A29 Gene 3'-UTR and Androgen Level of Muscle Tissue in Xiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3199-3209.

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Association Analysis Between Structural Variation of CYP3A29 Gene 3'-UTR and Androgen Level of Muscle Tissue in Xiang Pigs

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