湖羊SRP68基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.08.016

Abstract

Abstract: 【Objective】 This experiment was aimed to investigate the expression of signal recognition particle 68 (SRP68) gene of different tissues in Hu sheep at different months,and analyze the effect of its polymorphism on the growth traits in Hu sheep,in order to find the molecular markers significantly related to the growth traits in sheep.【Method】 The expression of SRP68 gene in heart,liver,spleen,lung,kidney and longissimus dorsi muscle of Hu sheep was detected by Real-time quantitative PCR,as well as in the longissimus dorsi muscle of Hu sheep at birth,45-day,4-month-old and 6-month-old.The polymorphism site of SRP68 gene was detected by Illumina OvineSNP 50 BeadChip in 3 024 Hu sheep.The association between polymorphism site of SRP68 gene and the growth traits of 1 974 Hu sheep was analyzed by SPSS 26.0 software.【Result】 SRP68 gene was expressed in different tissues of Hu sheep,and the expression of longissimus dorsi muscle and heart in Hu sheep at 45-day and 4-month-old were extremely significantly higher than that of other tissues (P＜0.01),and the expression of longissimus dorsi muscle at 6-month-old was extremely significantly higher than that of other tissues (P＜0.01).There was moderate polymorphism at rs421715384 G＞A of SRP68 gene in Hu sheep population (0.25＜PIC＜0.5).The birth weight and 2-month-old weight of AA and GG genotypes were significantly higher than that of GA genotype (P＜0.05).The 5-month-old body height of AA genotype was significantly higher than that of GA and GG genotypes (P＜0.05),and the 6-month-old body weight,tube circumference and chest width of GG genotype were significantly higher than that of AA genotype (P＜0.05).【Conclusion】 SRP68 gene was closely related to the muscle development of Hu sheep,and rs421715384 G＞A could be used as a potential molecular marker for growth traits in Hu sheep,which could provide a reference for molecular breeding in Hu sheep.

Key words: Hu sheep; SRP68 gene; tissue expression; association analysis

CLC Number:

S813.3

BI Yazhen, SHANG Mingyu, XIONG Jinke, HU Wenping, HE Jianning, ZHANG Li. Polymorphism of SRP68 Gene and Its Association with Growth Traits in Hu Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3189-3198.

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Polymorphism of SRP68 Gene and Its Association with Growth Traits in Hu Sheep

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