Abstract: 【Objective】 Based on bioinformatics analysis,the biomarker genes of Porcine reproductive and respiratory syndrome virus (PRRSV) infection were analyzed,and the composition identification and network pharmacology of the Yinhua Gancao decoction were conducted to explore the mechanism of the treatment of PRRSV by the Yinhua Gancao decoction.【Method】 Transcriptome data of PRRSV infection in different tissues or bodies were collected using high-throughput gene expression (GEO) database,differentially expressed genes were screened using GEO2R online software,protein interaction network was constructed using STRING software,and core proteins were screened using CytoNAC software.GO function and KEGG pathway enrichment analysis were used to determine the changes of core protein signaling pathways and biological functions after infection.Ultra high performance liquid chromatography time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to identify the components of the Yinhua Gancao decoction,and the active ingredient screening and target prediction were carried out by the SwissTargetPredication database,and the prevention and control mechanism of the Yinhua Gancao decoction for PRRSV infection was further predicted by network pharmacology.【Result】 A total of 4 476 differentially expressed genes after PRRSV infection were identified by 5 groups of gene chips in GEO database,and 164 core proteins were screened by protein interaction analysis.After PRRSV infection,the main genetic characteristics of the non-conventional ribosomal protein S27a (RPS27A),ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52) and RPS family were changed,and the biological function changes were biosynthesis process and ribosome structure,etc.The signal transduction pathways included autophagy,iron death and apoptosis.The Yinhua Gancao decoction contained a total of 70 effective components (isoliquiritigenin,formononetin,etc.),which act on 189 genes of PRRSV infection,involving apoptosis,cell senescence and other signaling pathways.Its core targets against PRRSV infection included tumor suppressor protein 53 (TP53),extracellular signal-regulated kinase 1 (MAPK1),tumor necrosis factor (TNF),MAPK14,etc.【Conclusion】 PRRSV infection might cause autophagy,iron death and apoptosis,and the active components of the Yinhua Gancao decoction might resist PRRSV infection by regulating genes such as TP53,TNF,CASP3 and STAT3,and cell cycle and apoptosis signaling pathways.

Key words: Porcine reproductive and respiratory syndrome virus (PRRSV); GEO database; Yinhua Gancao decoction; UPLC-Q-TOF-MS