水貂犬瘟热病毒LD-1株的分离与鉴定

Abstract

Abstract: Canine distemper virus (CDV) was detected positive by RT-PCR from a suspected CDV infected mink liver tissue. No mink parvovirus was detected from it. The virus was inoculated to 3 kinds of cells including primary CEF cells, Vero cells and DF1 cells for virus isolation. It was successfully isolated from Vero cells through the passage culture by optimizing the cells culture condition, and showed the dew shape typical CPE. The isolated virus was identified by RT-PCR, sequencing of the PCR products, serum neutralization test, indirect immunofluorescence test, virus inclusion body examination and other methods. The results showed that CDV was positive. It was proved that the isolated virus was a mink CDV, named CDV LD-1 strain.

Key words: canine distemper virus; virus isolation; identification

ZHUANG Jin-qiu, MEI Jian-guo, WANG Jin-liang, ZHANG Qian, SHEN Zhi-qiang, DING Zhuang. Isolation and Identification of Mink Canine Distemper Virus LD-1 Strain[J]. .

References

1 王好，张宇，钱爱东. 犬瘟热诊断方法的研究进展［J］. 畜牧与饲料科学，2009，30（10）：67～69.
2 王秀东. 致病性犬瘟热病毒的分离鉴定及其H基因的序列分析［D］. 北京：中国农业科学院，2010.
3 王金良，沈志强，管宇，等. 犬瘟热病毒RT-PCR检测方法的建立［J］. 动物医学进展，2006，27（12）：82～84.
4 庄金秋，梅建国，刘晓倩，等. 部分禽源生物制品外源病毒的检测［J］. 动物医学进展，2011，33（4）：121～125.
5 庄金秋，梅建国，沈志强. 犬瘟热病毒实验室检测方法研究进展［A］. 中国畜牧兽医学会兽医公共卫生学分会第三次学术研讨会论文集［C］.2012.
6 庄金秋，王金良，梅建国,等. 猪流行性腹泻病毒SDbz株的分离与鉴定［J］. 中国兽医学报，2013a，33（5）：650～653.
7 庄金秋，梅建国，姚春阳，等. CDV免疫蛋鸡血清抗体与卵黄抗体的消长规律及其相关性分析［J］. 家畜生态学报，2013b，34（5）：21～24.
8 张淼，刘清彪，刘宗架，等. 犬瘟热病原学研究进展［J］. 中国畜牧兽医，2009，36（11）：162～166.
9 杨井泉，韩猛立，朱艳平，等. 犬瘟热病毒巢式RT-PCR检测方法的建立及初步应用［J］.新疆农业科学，2011，48（6）：1098～1103.
10 林初文，庄金秋，陈金龙，等. 鸭黄病毒SDbz株的分离与初步鉴定［J］. 中国畜牧兽医，2013，40（4）：190～194.
11 宗春苗. 水貂犬源犬瘟热病毒的分离鉴定及H基因的序列分析［D］.长春：吉林农业大学，2007.
12 胡勇，吴胜昔，蔡家利，等. 抗犬瘟热病毒单克隆抗体杂交瘤细胞株的建立［J］. 安徽农业科学，2008，36（12）：4992～4993.
13 黎映胜. 犬瘟热诊断方法的研究进展［J］. 甘肃畜牧兽医，2009，39（6）：43～46.
14 Boucher C E, Bragg R R, Albertyn J. An alternative method for the establishment of virulence of Newcastle disease virus isolates［J］. Afri J Microbio Res，2010，4（21）：2313～2317.

[1]	ZHAO Jing, CHU Ying, LUO Jun, GUO Xiaofeng. Construction and Immunogenicity of Recombinant Rabies Virus with Canine Distemper Virus H Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4776-4785.
[2]	LIU Jian, BAI Yilan, JU Houbin, PAN Zihao, YU Xiangqian, YANG Xianchao, ZHU Xiaoying, GE Feifei, GE Jie, YU Huiru, TAO Tiangusheng, WANG Jian, ZHAO Hongjin. Genomic Sequencing Analysis of SH202003 Strain of Canine Distemper Virus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2559-2568.
[3]	WANG Zhaoyang, JIANG Yajun, LIU Xueting, LIN Weidong, XIN Ting, HOU Shaohua, GUO Xiaoyu, ZHU Hongfei, JIA Hong. Construction and Screening of Phage Library Against VHH Antibody of Canine Distemper Virus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1685-1693.
[4]	YU Zekun, DUAN Xiaoxiao, ZHI Yong, LUAN Zhifang, SHAN Xueqiang. Construction of Vero Cell Line Stably Expressing Nectin4 Receptor of Canine Distemper Virus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3334-3342.
[5]	CHEN Meirong, LIN Weidong, SONG Tianqi, XIN Ting, GUO Xiaoyu, HUANG Kehe, JIA Hong. Expression and Identification of H Gene of CDV BJ16B2 Strain in Insect Baculovirus Expression System [J]. , 2018, 45(8): 2041-2048.
[6]	ZHAO Xueli, LIU Yi, BAN Fuguo, YAN Ruoqian, WANG Huajun, WANG Shujuan, MA Zhenyuan, WANG Dongfang. Establishment and Application of A Double TaqMan MGB Real-time PCR Assay for Detection of Canine Distemper Virus and Canine Parvovirus [J]. , 2018, 45(8): 2086-2094.
[7]	ZHANGX Xin, HUANG Jian, ZHANG Ping, YANG Xiaonong. Molecular Epidemiologic Investigation on Infections of Canine Distemper Virus and Canine Respiratory Coronavirus in Pet Dogs in Chengdu Area [J]. , 2018, 45(2): 486-492.
[8]	LIU Li, HAO Fu-xing, YUAN Wei-feng, FENG Jia-wang. Expression, Identification and Subcellular Localization of V Protein of Canine Distemper Virus [J]. , 2016, 43(7): 1723-1728.
[9]	SUN Yan-gang, ZHAO Jian-jun, BAI Xue, NIU Deng-yun, SHAO Xi-qun, HU Bo, LIU Hao, ZHANG Hai-ling, ZHANG Lei, WANG Yang, MA Fan-shu, YAN Xi-jun. Cloning and Genetic Variability Analysis of Single Peptide Gene Regions of Fusion Protein of Canine Distemper Virus from Minks,Foxes and Raccoon Dogs [J]. , 2015, 42(4): 838-846.
[10]	WANG Xi-xi, WANG Feng-xue, CHENG Shi-peng, WEN Yong-jun. Research Progress on Infection Mechanism and Diagnostic Methods of Canine Distemper Virus [J]. , 2015, 42(3): 757-763.
[11]	QUAN Chuan-song, JIANG Qian, YU Zuo, LI Bo-tao, LI Lian-feng, QU Lian-dong. Expression of Hemagglutinin of Canine Distemper Virus in the Insect Cell and its Antigenic Analysis [J]. , 2014, 41(8): 25-29.
[12]	SUN Yuan-yuan, ZHAO Peng, LIU Jun, WANG Yun, QIAN Ke. Development and Application of Duplex PCR for Canine Distemper Virus and Canine Coronavirus Detection [J]. , 2014, 41(3): 99-103.
[13]	SUN Jian-tao, HOU Shao-hua, YU Ping, ZHU Hong-fei, LIANG Qian, HAO Zhi-ming. Study on the Construction and Expression Characteristics of Canine Distemper DNA Vaccine Based on SFV-replicon [J]. , 2013, 40(9): 96-100.
[14]	ZHUANG Jin-qiu, MEI Jian-guo, YAO Chun-yang, SHEN Zhi-qiang, DING Zhuang. Dynamics Research of Serum and Egg Yolk Antibody of Laying Hens Immuned by Canine Distemper Virus [J]. , 2013, 40(9): 214-217.
[15]	YUAN Wei-feng, JIA Hong, YU Ping-ping, HOU Shao-hua, GUO Xiao-yu, SHI Li-jun, ZHAO Zhan-zhong, ZHU Hong-fei. Preparation and Identification of Monoclonal Antibodies against Recombinant Truncated P Protein of Canine Distemper Virus [J]. , 2013, 40(4): 22-25.

Isolation and Identification of Mink Canine Distemper Virus LD-1 Strain

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments