犬瘟热病毒V蛋白的表达、鉴定及亚细胞定位

doi:10.16431/j.cnki.1671-7236.2016.07.010

Abstract

Abstract:

In order to study the function of canine distemper virus (CDV) V protein,V gene fragment was inserted into the pGEX-6p-1 vector,and then pGEX-6p-1-CDV-V recombinant expression plasmid was obtained.Purified V protein immunized BALB/c mice to prepare the positive serum.Meanwhile,to confirm the distribution of V protein and subcellular localization with confocal laser technology,a eukaryotic expression recombinant plasmid pcDNA3.1-CDV-V was built,then transfected into Vero cells.The results showed that V protein was successfully expressed and the positive serum was prepared.The recombinant plasmid pcDNA3.1-CDV-V was expressed in Vero cells and mainly expressed in cytoplasm.The results laid a function for functional studies of CDV V protein.

Key words: canine distemper virus; V protein; prokaryotic cell; subcellular localization

CLC Number:

S852.65⁺5

LIU Li, HAO Fu-xing, YUAN Wei-feng, FENG Jia-wang. Expression, Identification and Subcellular Localization of V Protein of Canine Distemper Virus[J]. , 2016, 43(7): 1723-1728.

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Expression, Identification and Subcellular Localization of V Protein of Canine Distemper Virus

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