Abstract: The lung samples with pneumoniae were collected from Tibetan sheep. Bacterial isolation and identification were performed by culture and PCR methods. Antibiotic sensitivity of the isolates was determined, so as to provide useful information for treatment of the disease. The results showed that the isolated bacteria were gram-positive and chain-forming coccus-shaped proven to be Streptococcus by specific PCR, clone and sequence. It was also demonstrated that the isolates were sensitivity to most of antibiotics, especially for trimethoprim-sulfamethoxazole, enrofloxacin, gentamicin and tetracycline. The results obtained in this study provided useful basis for control and treatment of Streptococcus infection of sheep in Tibetan.

Key words: sheep lung; Streptococcus; identification; drug resistance