鄂尔多斯细毛羊前后盘吸虫病病原分子鉴定及驱虫药物筛选

doi:10.16431/j.cnki.1671-7236.2025.04.032

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the species of Paramphistomum in Ordos fine-wool sheep and screen out ideal anthelmintic drugs.【Method】 Based on the preliminary diagnosis of paramphistomiasis by fecal egg examination,the Paramphistomum in rumen of Ordos fine-wool sheep were collected through necropsy for morphological and molecular biological identification.Three parasites were randomly selected to extract DNA,and then the internal transcribed spacer 2 (ITS-2) of ribosome was amplified by PCR.After sequencing the amplified products,BLAST alignment analysis was performed in NCBI database,and the phylogenetic tree was constructed.Niclosamide,nitroclofene,rafoxanide and 3% trichlorfon combined with nitroclofene were respectively used to conduct a comparative test on the anthelmintic effects in sheep infected with paramphistomiasis.【Result】 The band size of 441 bp was obtained by PCR amplification of ITS-2 gene,which was consistent with the target gene band.The sequence similarity between the three parasites and the ITS-2 (KJ459936.1) from China was 99.3%,99.3% and 99.8%,respectively.The results of phylogenetic analysis showed that this Paramphistomum was in the same large branch as Paramphistomum from China (KP341671),Ireland (AB973398),Argentina (HM209066) and Germany (MZ532797),and was in a small branch with Paramphistomum cervi,indicating that the Paramphistomum obtained in this study was Paramphistomum cervi.In the anthelmintic test,the egg reduction rates in niclosamide,nitroclofene,rafoxanide and 3% trichlorfon combined with nitroclofene groups were 44.00%,26.73%,23.00% and 90.80%,respectively.【Conclusion】 The Paramphistomum parasitizing in Ordos fine-wool sheep in Wushen Banner,Inner Mongolia was Paramphistomum cervi,and the treatment with a combination of 3% trichlorfon and rafoxanide could achieve a better anthelmintic effect.

Key words: Ordos fine-wool sheep; Paramphistomum; molecular biological identification; anthelmintic effect

CLC Number:

WURI Lige, LI Na, GERILE Gelaba, HASI Tuya, ZHANG Jingjing, HOU Bin, HASI Surong. Molecular Identification of the Pathogen of Paramphistomiasis in Ordos Fine-wool Sheep and Screening of Deworming Drugs[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1807-1814.

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Molecular Identification of the Pathogen of Paramphistomiasis in Ordos Fine-wool Sheep and Screening of Deworming Drugs

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