Abstract: The ovine pulmonary adenomatosis (OPA) is composed of β-reverse transcription virus jaagsiekte sheep retrovirus (JSRV) caused by a tumor infectious disease, the disease has a long incubation, spreads through the respiratory tract, the winter of captive breeding disease incidence rate is high, at present there are no treatment measures, the mortality rate is 100%. The continuing presence of OPA is a potential threat for sheep production and has caused great economic losses, serious harm to the healthy development of sheep industry. Therefore, early precise diagnosis on OPA is the premise of the disease prevention and control, particularly in the entry exit inspection and quarantine process of import and export of sheep JSRV detecting is very important. With the development of molecular biology techniques, JSRV molecular biology detection method has constant innovation and improvement. This review will focus on the detection method of JSRV, polymerase chain reaction (PCR), nucleic acid hybridization, enzyme linked immunosorbent assay (ELISA), loop mediated isothermal amplification (LAMP) survey research methods in recent years, to provide reference methods for the detection of JSRV and further development of the research on new JSRV detection methods for rapid, accurate and suitable in the entry and exit inspection and quarantine process.

Key words: ovine pulmonary adenomatosis; jaagsiekte sheep retrovirus; detection method