含非洲猪瘟病毒核酸序列病毒样颗粒的研制及其在检测方法中的应用

doi:10.16431/j.cnki.1671-7236.2017.11.025

Abstract

Abstract:

African swine fever (ASF) is an infectious disease caused by the African swine fever virus (ASFV). In order to ensure the accuracy and reliability of the test results,it is necessary to develop positive standard control products used in the kit. The study was aimed to develop the virus-like particles containing African swine fever virus (ASFV) nucleic acid sequence and its application in detection method. Fristly,the full-length gene fragment of p72 gene was amplified, and the ASF DNA virus-like particles containing p72 gene was constructed using insect baculovirus system. In order to further validate the reliability of the virus-like particles in the application of the method,DNA nucleic acid was extracted simultaneously with the cultured virus and infected tissue sample and applied in the Real-time quantitative PCR method. The results showed that the virus-like particles prepared by this study could replace the ASFV as a positive control product in the Real-time quantitative PCR method, and could act as quality control during nucleic acid extraction process. In the fluorescent PCR detection kit, the lowest packaging concentration of virus-like particles was 10² TCID₅₀. Further studies had shown that the virus-like particles were also suitable for common PCR and LAMP detection methods, the minimum concentration were 10³ and 10¹ TCID₅₀, respectively. The results of this study would be important for the ASF detection method, promoting the application of the method and ensuring the accuracy and reliability of the test results.

Key words: African swine fever (ASF); reference substance; virus-like particle; detection methods

CLC Number:

S852.65

FENG Chun-yan, DU Fang-yuan, LIU Dan-dan, Pershin Andrey, WANG Cai-xia, ZHANG Yong-ning, WU Shao-qiang, LIN Xiang-mei. Development of Virus-like Particles Containing African Swine Fever Virus Nucleic Acid Sequence and Its Application in Detection Method[J]. , 2017, 44(11): 3287-3293.

References

[1] Hess W R. African Swine Fever Virus[M].Springer Vienna,1971.
[2] Bastos A D S,Fasina F O,King D P. African swine fever virus[J]. Manual of Security Sensifive Microbes and Toxins,2014,2:579-587.
[3] Costard S,Mur L,Lubroth J,et al. Epidemiology of African swine fever virus[J].Virus Research, 2013,173(1):191-197.
[4] Dixon L K,Chapman D A G,Netherton C L,et al. African swine fever virus replication and genomics[J].Virus Research,2013,173(1):3-14.
[5] Gabriel C,Blome S,Malogolovkin A,et al.Characterization of African swine fever virus Caucasus isolate in European wild boars[J].Emerging Infectious Diseases,2011,17(12):2342.
[6] Costard S,Wieland B,De Glanville W,et al. African swine fever:How can global spread be prevented[J].Philosophical Transactions of the Royal Society B:Biological Sciences, 2009,364(1530):2683-2696.
[7] Yáñez R J, Rod?guez J M,Nogal M L,et al. Analysis of the complete nucleotide sequence of African swine fever virus[J].Virology,1995,208(1):249-278.
[8] Blome S,Gabriel C,Beer M. Pathogenesis of African swine fever in domestic pigs and European wild boar[J].Virus Research,2013,173(1):122-130.
[9] Jori F,Bastos A D S. Role of wild suids in the epidemiology of African swine fever[J].Eco Health, 2009,6(2):296-310.
[10] Nurmoja I,Petrov A,Breidenstein C,et al. Biological characterization of African swine fever virus genotypeⅡ strains from north-eastern Estonia in European wild boar[J].Transboundary and Emerging Diseases,2017,Epub ahead of print.
[11] Galindo I,Alonso C. African swine fever virus:A review[J].Viruses,2017,9(5):103.
[12] 张瑞.人乳头瘤病毒基因分型检测参考物质及其应用研究[D].北京:北京协和医学院中国医学科学院,2013.
[13] 李金明.标准物质在乙型和丙型肝炎病毒核酸检测标准化中的重要性[J].中华检验医学杂志,2007,30(8):850-852.
[14] 王露楠,邓巍,申子瑜,等.乙型肝炎病毒DNA标准物质的研究[J].中华肝脏病杂志,2007,15(2):107-110.
[15] 黄邱朝,丁庆.抗DNA阳性参考血清的制备[J].现代免疫学,1993,3:63-64.
[16] 孙敏,梁成珠,王群,等.含非洲马瘟病毒部分核酸序列的病毒样颗粒的研制[J].畜牧兽医学报,2015,46(4):637-643.
[17] 王彩霞,林祥梅,吴绍强.非洲猪瘟环介导恒温扩增(LAMP)快速检测方法的建立[A].中国畜牧兽医学会2008年学术年会暨第六届全国畜牧兽医青年科技工作者学术研讨会论文集[C].2008.
[18] 李芹,丁壮.病毒样颗粒研究进展[A].中国畜牧兽医学会动物传染病学分会第十六次学术研讨会[C].2015.
[19] 温和心,龙英全,蒋荣华,等. RNA标准物病毒样颗粒表达系统的构建及其特性研究[J].动物医学进展,2014,3:6-10.
[20] 韦永龙,李轶女,张志芳,等.杆状病毒表达系统及其应用进展[J].生物技术通报,2010,10:1-7.
[21] 刘拂晓,柳增善,王志亮.杆状病毒表达系统——有效的VLP构建工具[J].生物技术通报,2012,6:25-31.
[22] 曹蕾,伊瑶,宋敬东,等.杆状病毒表达EV71病毒样颗粒的装配、制备纯化与鉴定[J]. 病毒学报, 2012,3:201-206.
[23] 刘立颖,钱渊.病毒样颗粒在昆虫杆状病毒表达体系中的表达、组装及其应用研究[J]. 国际病毒学杂志, 2008, 25(2):46-51.

Development of Virus-like Particles Containing African Swine Fever Virus Nucleic Acid Sequence and Its Application in Detection Method

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHENG Jing, LIU Wenxiao, WANG Xiaoyue, WANG Liping, JIANG Bo, LI Yongqing. Research Progress on Development of Diagnostic Methods of African Swine Fever [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1985-1993.
[2]	GUO Luanying, LI Jialei, JIN Qinqin, MA Jun, LIU Quan. Research Progress on Bovine Hepacivirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1994-2000.
[3]	CHI Shanshan, JIA Weijuan, HE Yunjiang, MENG Qinglei, CHEN Yunjiao, WANG Xueli. Research Progress on Newcastle Disease Virus-like Particles [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1507-1515.
[4]	WU Shuaibin, CUI Zehua, LIAO Xiaoping, LIU Yahong, SUN Jian. Research Progress on the Detection Methods of Carbapenemase-producing Enterobacteriaceae [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1072-1083.
[5]	WU Mingjie, XIA Linya, WANG Lei, ZHANG Ning, LUO Guoliang, YIN Yuhe, WU Congmei. Prokaryotic Expression of Mink Enteritis Parvovirus VP2 Gene and Assembly of Virus-like Particles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 433-442.
[6]	WANG Yong, HAN Yuqi, LU Minghua. Research Progress on Detection Methods of Drug Residues of β-receptor Agonist [J]. , 2019, 46(8): 2478-2487.
[7]	HAN Wei, ZHOU Jianmin, ZONG Shucheng, YANG Weiwei, PAN Jingxue. Research Progress of Swine Japanese Encephalitis Vaccine [J]. , 2019, 46(8): 2488-2495.
[8]	WANG Xi, LI Guopan, CHEN Qingqing, XU Baojuan, RONG Jun. Purification and Identification of Porcine Circovirus Type 2 Virus-like Particles [J]. , 2019, 46(6): 1792-1800.
[9]	LIU Hanping. Construction and Identification of Swine O-type Foot-and-mouth Disease Virus-like Particles [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3350-3357.
[10]	WANG Yong, GONG Yong, LU Ming-hua. Research Progress on Detection Methods of Fluoroquinones Residues [J]. , 2017, 44(8): 2509-2516.
[11]	HAN Xiao-ya, CHEN Dong-mei, WANG Yu-lian, TAO Yan-fei, DONG Guo-liang, PENG DA-peng, YUAN Zong-hui. Research Progress on Antibodies Rapid Detection Technology for Veterinary Synthetic Antibacterial Agents [J]. , 2017, 44(6): 1869-1876.
[12]	WANG Ming-ming, XU Na, TANG Yun, HANG Ye, LIU Zhen-jiang, DU Dao-lin. Advances on Detection Methods for Residues of Nitrofurans in Food [J]. , 2016, 43(8): 2202-2207.
[13]	ZHU Yu-fei, XIE Shu-yu, CHEN Dong-mei, WANG Yu-lian, PAN Yuan-hu, PENG Da-peng, TAO Yan-fei, YUAN Zong-hui. Research Progress on Detection Methods of Feed Additives [J]. , 2016, 43(7): 1785-1791.
[14]	PEI Ye-chun, AN Xiao-rong, HOU Jian, CHEN Yong-fu, YAN Feng-xiang, GUAN Hong, WEI Shuang-shuang, WANG Da-yong. Prokaryotic Expression of Fusion Gene of Cat Recombinant Allergen Fel d 1 with Hepatitis B Core Antigen [J]. , 2016, 43(11): 2826-2833.
[15]	DENG Jun-hua, LIN Xiang-mei, ZHANG Yong-ning, WANG Cai-xia, WU Shao-qiang. Construction and Quantitative Analysis of His-tagged Bluetongue Virus-like Particles [J]. , 2014, 41(9): 68-73.