猪伪狂犬病病毒LC株的分离鉴定及其重要功能基因的进化分析

doi:10.16431/j.cnki.1671-7236.2017.11.024

Abstract

Abstract:

To find out the reason of the reproductive failure in pregnant sows in a hoggery in Guangdong, the mixture with brain, lymph nodes and lungs of farm abortion stillbirth were identified by PCR, virus isolation and culture, tissue culture infective dose (TCID₅₀) assay, homology and phylogenetic analysis of the important functional genes (gB, gC, gD and gE) and animal test. The results showed that the mixture was proved to be porcine pseudorabies virus (PRV) positive samples. The typical cytopathogenic effect was induced in the third passage of Vero cell and the titer of the fifth passage was 10^-6.8/0.1 mL. The sequence analysis and phylogenetic relationship of gB, gC, gD and gE genes showed that it was a Chinese PRV variant, which was named as LC strain. The typical pseudorabies clinical and pathological symptoms were presented in 12-week-old piglets inoculated with LC strain. The results demonstrated that a local pseudorabies virus had been isolated, suggesting that the Bartha-K61 vaccine was not fully effective for controlling the current epidemic of pseudorabies in China.

Key words: porcine; pseudorabies virus (PRV); isolation and identification; phylogenetic analysis

CLC Number:

S852.65⁺9.1

LIU Zhi-cheng, LI Xin-jian, ZHANG Jian-feng, SHEN Hai-yan, SUN Jun-ying, ZHANG Chun-hong. Isolation, Identification and Phylogenetic Analysis of Important Functional Genes of Porcine Pseudorabies Virus LC Strain[J]. , 2017, 44(11): 3278-3286.

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Isolation, Identification and Phylogenetic Analysis of Important Functional Genes of Porcine Pseudorabies Virus LC Strain

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