6个绵羊品种微卫星多样性分析

Abstract

Abstract: To detect the genetic diversity of Black bone sheep in Lanping and its genetic relationship with the other five sheep breeds (Hu sheep, Small-tail Han sheep, Dorper sheep, Texel sheep and hornless Dorset sheep), nine microsatellite loci were selected to study a total of 117 individuals, PCR amplification products of the microsatellite loci were resolved by native-polyacrylamide gel electrophoresis, and POPGEN32 software was used to calculate genetic parameters. The results showed that nine microsatellite loci were high polymorphism. The mean polymorphism information content (PIC) in each sheep population was 0.7211 (Hu sheep), 0.6780(Dorset sheep), 0.6492 (Small-tail Han sheep), 0.6479 (Black bone sheep), 0.5930 (Texel sheep) and 0.5728 (Dorper sheep), respectively. Among the six breeds, the mean heterozygosity (H) of Black bone sheep was highest (0.9543), while Dorper sheep was the lowest (0.2556). UPGMA cluster analysis showed that Hu sheep and Small-tail Han sheep clustered into a first class; the later was Black bone sheep. The further genetic distance was between Black bone sheep and the three introduced species. These results suggested that Black bone sheep might be crossed with Hu sheep or Small-tail Han sheep to improve fertility, and crossed with the introduced species to improve meat performance.

Key words: sheep; microsatellite; genetic diversity; genetic distance

WANG Ya-lei，LI Jing-xin，MAO Da-gan，WANG Hui-li，LI Yin-xia，QIAN Yong，MENG Chun-hua，CAO Shao-xian. Analysis of Genetic Diversity on Microsatellite in Six Sheep Breeds[J]. .

References

1 仲涛，马月辉，关伟军，等.10个绵羊品种的微卫星DNA多态性研究［J］.畜牧兽医学报, 2008, 39(5)：555～561.
2 张爱玲，马月辉，陈秀梅，等.不等微卫星座位数目对Nei氏标准距离估计精确度的影响［J］.畜牧兽医学报，2005，36(5)：431～433.
3 杨章平，常洪，孙伟，等.7个绵羊微卫星DNA标记在绵(山)羊种群中的多态性检测［J］.西北农林科技大学学报：自然科学版，2004，32(12)：69～74.
4 娄渊根,刘丑生,刘建学，等. 9个绵羊品种微卫星DNA遗传多样性分析［J］. 中国畜牧兽医, 2011, 38(10): 106～112.
5 耿岩，杨章平，常洪，等.中国蒙系6个绵羊品种的遗传分化和基因流［J］.扬州大学学报，2007，28(3)：22～26.
6 柴文琼，成述儒，靳建华，等.5个绵羊群体微卫星多态性分析［J］.扬州大学学报，2012，33(1)：39～42.
7 储明星.利用分子育种技术选育肉用多胎绵羊新品系［J］.中国农业科技导报，2004，6(6)：15～20.
8 Beckman J S, Weber J L. Survey of human and rat microsatellites ［J］.Genomics,1992,12(4):627～631.
9 Botstein D, White R L, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms ［J］. Am J Hum Genet,1980,32(3): 314～331.
10 Gupta P K, Balyan I S, Sharma P C, et al. Microsatellites in plants: A new class of molecular markers ［J］.Current Science,1996,70(1):45～54.
11 Hamada H, Petrino M G, Kakunaga T. A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes ［J］.Proc Natl Acad Sci, 1982, 79(21): 6465～6469.
12 Hines H C, Zikakis J P, Haenlein G F, et al. Linkage relationships among loci of polymorphism in blood and milk of cattle ［J］. J Dairy Sci,1981,64(1):71～76.
13 Sun W, Chang H, Musa H H, et al. Microsatellite-based genetic differentiation and phylogeny of sheep breeds in Mongolia sheep group of China ［J］. Agricultural Sciences in China, 2011, 10(7):1080～1087.
14 Takezaki N, Nei M. Genetic distance and reconstruction of phylogenetic trees from microsatellite DNA ［J］.Genetics, 1996,144(1): 389～399.
15 Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers ［J］. Nuleic Acids Res,1989,17(16):6463～6467.

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Analysis of Genetic Diversity on Microsatellite in Six Sheep Breeds

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